What is an appropriate internal standard for lactate/pyruvate analyses of brain microdialysates?

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Top of pagePrevious messageNext messageBottom of pageLink to this message  By sufulls on Tuesday, May 18, 1999 - 11:52 am:

I am looking for an internal standard that could be used in the HPLC analysis of lactate and pyruvate from brain microdialysates. We are using BioRad's column (HPX-87H)for the analysis of these organic acids, but have not been able to find an internal std that is not also in our physiological samples.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Tom Jupille on Saturday, May 22, 1999 - 02:01 pm:

You might want to look at one of the perfluorinated acids. These are unlikely to be endogenous to your samples. TFA (trifluoroacetic) probably has too little retention to be useful (but I'm not certain of that!), but HFBA (heptafluorobutyric) might work. You would have to establish that it doesn't coelute with any endogenous compounds, but then that's true of *any* internal standard.

Hope this helps!

-- Tom Jupille / LC Resources


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