Method Validation - RSD%

Chromatography Forum: LC Archives: Method Validation - RSD%
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Jaime on Wednesday, August 11, 2004 - 07:43 am:

When determining the precision of a method, do you make 4 or more sample preparation and one injection each, or one sample preparation and 4 or more injection from that preparation. Or is it 4 sample preparation or more and 4 0r more injection per sample preparation.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By David Blais on Wednesday, August 11, 2004 - 08:52 am:

Jaime,

Method precision should be multiple sample preps and can be one injection each. Whenever you perform multiple injections from one preparation, you are looking at autosampler precision. Typically when validating a method I prepare six sample preparations and make one injection of each.

Hope this helps.
-David


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Jaime on Wednesday, August 11, 2004 - 11:26 am:

Thank you David.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Leadna N Brigola on Friday, August 13, 2004 - 12:49 am:

Jaime,
Agree with David.Method precision is done using at least 6 replicate preparations of the sample and single injection of each replicate. for system precision its 6injections of 1 solution.
Hope this helps.
Leadna


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