I have tried about 2 or 3 methods for derivitising sugar phosphates and nucleotides but none of them seems to work properly. The methodology I have is rather old (1960-1970).I have tried (hexamethyldisilazane:trimethylchlorosilane)(2:1) HMDS:TMCS in pyridine and HMDS:TMCS using dimethyl sulfoxide and cyclohexane. Does anyone know a method or a spesific reference for the derivatisation of both sugar phosphates and nucleotides? Any help would be mostly appreciated.
Thank you in advance.

You might try contacting Pierce Chemical, they used to be a great source for derivitizations. I would suggest using TMSI (trimethylsilylimidazole) as it works with small amounts of water present and I believe is the reagent of choice for sugars. They are on the web at www.piercenet.com also. Hope this little bit helps.