I am using an Haysep-R column and injecting a 2ul sample which contains water. I have experienced an enormous tail in my water peak and was wondering how to minimized such a peak.
By Bill Jenko on Tuesday, October 31, 2000 - 07:45 pm:
1.) Raise the temperature. Resolution of your peaks of interest may suffer
2.) Try a different porous polymer. Water tends to tail less (being retained far less) on Hayes P and Q than on N,R & S in my experience. Depending on what you are trying to measure, it may even be possible to put your peak(s) of interest in front of water, using Hayes T, and backflush the water.
3.) Reduce the injected volume. Any chance you can shoot 0.5 ul and still have adequate sensitivity?
What are you trying to separate from the water peak?
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