Does anyone have suggestions of a good reference book on sample derivatizations for HPLC (both for UV and fluorescence detection)?
I am looking for a procedure to derivatize an alcohol so it can be run in a LC with fluorescence detection.
Thank you for your input.
By Jonathan Green on Monday, August 23, 1999 - 04:44 am:
Although their probes are not specifically for HPLC derivatization, a company called Molecular Probes in Eugene, Oregon has the most comprehensive catalog of fluorescent probes I've ever seen. They have a web page at www.probes.com and their customer service folks are most helpful.
By hinsbarlab on Monday, August 23, 1999 - 06:53 am:
Regis Technologies, Inc. sells HPLC derivatization reagents and also provides procedures and references in their catalog. Their telephone number is 800-851-2436.
Hinsbar Laboratories, Inc.
By HW Mueller on Tuesday, August 24, 1999 - 07:46 am:
Advanstar advertises a book: Handbook of Derivatization Reactions for HPLC, you may get more information at ...WWW.ams-europe.co.uk.
Older reviews are:
Hulshoff and Lingemann, in Molecular Luminescence Spectroscopy, Wiley, NY, 1985, p. 621 (vol. 77 of Chemical Analysis...)
Ohkura and Nohta, Fluorescence Derivatization in HPLC, Advan. Chromatogr., 29, 221 (1989)
Lingemann, et al, J. Liquid Chrom., 8, 789 (1985)
By Benjamin Esquivel on Tuesday, August 24, 1999 - 12:59 pm:
Try the book "Handbook of Derivatizations Reaction for HPLC' by G.Lunn and L.C. Hellwig (Wiley Interscience.
A good reagent to derivatize alcohols is Naphtoylimidazole (p 60).
By AmjadK on Wednesday, August 25, 1999 - 09:42 am:
Try the Handbook of derivatives for chromatography, 2nd Ed, Karl Blau and John Halket,
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