I am working in pesticide residue analysis. We are analysing about 35 pesticides using Luke method and determination with GC-ECD, we use HP-6890 with HP-PAS 1701 and PAS 5 capillary column. The problem is that we notice a transformation of p,p-DDT into p,p-DDD. When we injected p,p-DDT on GC-FID under the same conditions of temperature program and column types and also HPLC-UV we get only single peak for DDT. Please any response about the reason of such transformation will be appreciated.
By Anonymous on Thursday, October 3, 2002 - 09:10 am:
Your column and liner are very active, therefore you have problem with thermally labile components such as ddts and particularly endrin. You have to obtain a very low activity liner/column. Normally, endrin is a good test for activity or try cool oncolumn injection.
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