Has anyone had experience using the Merlin Microseal septum with SPME? I am skeptical of how many injections it will tolerate without developing leaks.
By Anonymous on Thursday, October 24, 2002 - 12:17 pm:
The Merlin Microseal leaks, and the insert with the product states that leak rates of several mL/min are normal. The way around this is to operate at a high split ratio, so that there is enough flow to overpower the leak, so that precision is not affected and GCs with electronic flow control do not give a carrier error. The SPME application with the LEAP autosampler may benefit from using the microseal because the insertion force is low, very important for the SPME needle. If you are doing manual SPME, the Merlin is not recommended for any manual injections due to the ease of damaging the duckbill sealing surface.
The Merlin Microseal does work well in high split applications, but I have performed over 400 injections with a regular septum without problems under the same type of high split conditions.
To summarize, if you can use a high split ratio for you analysis the Microseal will probably work fine, and is appropriate for the SPME application.
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