I've been asked to develop a method to determine the levels of CPC - cetylpyridinium chloride, in toothpaste and mouthwash formulations by HPLC. I am a little pressed for time, and more of a GCMS guy than HPLC.

I imagine I will use a C18 column, MeOH/PO4 aqueous buffer mobile phase, and UV detection. I think I should be able to find the CPC peak no problem, but will I need to do some type of SPE cleanup to separate the CPC from sorbitol, saccharin, glycerine etc.

It seems to me there has got to be some work done in this area already.

Can anybody help me get started?

Thanks,

Mike