By steven on Thursday, January 23, 2003 - 06:02 am:
Hi,
Recently, we run a standard containing Methamphetamine, using GMSD with HP5 column. However, the chromatograme showed 2 peaks with good separated baseline. The library matching showed both are methamphetamine too. The question is: do normal GCMSD column (HP5) able to separate methamphetamine enantiomers and causing 2 peaks seen on chromatogram?? Would be happy if anybody came across methamphetamine analysis and share the experience. Thank you very much.
Regards,
Steven
By B.Buglio on Monday, January 27, 2003 - 07:09 am:
Chiral recognition with a HP5 is unlikely. Are the
2 peaks of equal area?
By Benjamin on Monday, January 27, 2003 - 09:24 am:
Steven;
Is it possible that the two signals you are seeing are isomers of some other type? The MS would look very similar but the retention is likely to be different.
I agree with B. Buglio. HP-5 columns are not expected to resolve enantiomers. Silicone-based phases are not chiral unless they are mixed with some sort of derivatized cyclodextrin or other chiral recognition agent.
Look into what other type of isomer could be there, perhaps positional, geometric, etc.
Good Luck;
Benjamin