A need información of response factors for GC in triglycerides, a have the method but the response of mono-di and tri is not correct.
By Anonymous on Friday, July 25, 2003 - 01:38 am:
Even with on-column I found that I had to prepare calibration graphs of the mono, di and tri to do quantitative work on oils and fats.
If you are you using split/splitless you may be getting discrimination in the injection port because of the wide boiling range, which will make matters worse.
By Anonymous on Friday, July 25, 2003 - 02:42 am:
Thank you for your answer!!
I'm working with on-column,I don't have experience with calibration methods.
Could you help me ? Do you have any bibliography about this?
By Anonymous on Monday, July 28, 2003 - 03:29 am:
This paper may give you a start. Also have a word with the manufacturer of your GC or column. You are going to need standards of selected mono, di and triglyceride. I think that the LCGC magazine/site also has some calibration articles.
A RAPID GAS-CHROMATOGRAPHY METHOD FOR QUANTITATION OF FREE FATTY-ACIDS, MONOACYLGLYCEROLS, DIACYLGLYCEROLS, AND TRIACYLGLYCEROLS WITHOUT DERIVATIZATION
KWON SJ, LEE SY, CHO SW, RHEE JS
7 (10): 727-732 OCT 1993
Document type: Article Language: English Cited References: 8 Times Cited: 7
Quantitative analysis of mixtures of free fatty acids, monoacyl-, diacyl-, and triacylglycerols by a simple and rapid gas chromatography method has been developed. The analysis was carried out on a short capillary column without derivatization. When the triacontane was used as an internal standard, we obtained relative response factors for each lipid class with reproducibility and accuracy. This method could be applied for not only compositional analysis of fats and oils but also monitoring lipase reactions.
KOREA ADV INST SCI & TECHNOL,DEPT BIOTECHNOL,373-1 KUSONG DONG,TAEJON 305701,SOUTH KOREA
DOOSAN TECH CTR,KYONGGI DO 449840,SOUTH KOREA
By Himika Varma on Friday, March 26, 2004 - 07:05 am:
I came across this thread through a search engine while searching for methods on analysis of triglycerides in edible oils on HPLC - RI or GLC.
Could you please provide online links / references where I can locate the above methods?
Thanks & Regards