While preparing sililating darivative, adding 1 ml to 10 mg FA and injecting onto DB23, BP1 & DB1 capillary columns of J & W gives lots of tailing & the peaks are not sharp and comparable to methyl esters of fatty acids prepared by using methanol & thionyl chloride. please advise
I would also like to know what is the accuracy of internal standard method
By Anonymous on Tuesday, September 23, 2003 - 10:00 am:
I'd abandon the silation and use methyl esters.
By Anonymous on Wednesday, September 24, 2003 - 08:00 am:
You can do free fatty acids on FFAP phases and get excellent peak shapes. We use a ZB-FFAP routinely. It is less sensitive than FAMEs and so on - but if you don't need the sensitivity why not go for the least complicated option?
By Soap Guy on Wednesday, September 24, 2003 - 11:10 am:
I work for soap company - we usually make fatty acid methyl esters (boron trifluoride-methanol or sulfuric acid -methanol), but have also used TMS derivatization. What exactly are you trying to do?
By Anonymous on Friday, September 26, 2003 - 01:44 am:
From your description it sounds like you are not silylating your fatty acids.
By Soap Guy on Friday, September 26, 2003 - 12:00 pm:
We use BSTFA to derivatize for trimethylsilyl derivatives.