I am running on a packed glass column 6ft/2mm, 5% Carbowax 20 mesh.
I am injecting 5 ÁL of sample (diluted in water).
I see carry-over following standard for the next 6 blank injections.
I have tried adding a burn out to the end of the run, added syringe washes both post and pre sample injection.
I have a new needle, septum and jet tip. The carry over is significant (0.1-0.2% of the standard peak area). Does anybody have any additional ideas?
By Anonymous on Monday, May 17, 2004 - 01:58 pm:
It might help to know what it is that is carrying over.
By Carlos Teixeira on Tuesday, May 18, 2004 - 11:14 am:
Hello Anon- 01:39
Is your system fully clean (detector, injector, etc)?
By labcat on Tuesday, May 18, 2004 - 11:49 pm:
Dear anon., I had the same problem a couple of years ago. Look for the following discussion: "Injection carryover with Agilent autosampler" in this forum (Arch. June-Dec 2002). Hope it helps!