By ori951 on Monday, May 24, 2004 - 11:28 am:
i've got a colunm type CP WAX for polaire compound, recently i work with an aldehyde product
which is in equilibrium with its enol form; the nmr analysis confirmed that observation.
My question is: can we detect the enol form by GC
or these 2 product give one single commun peak?
By Anonymous on Monday, May 24, 2004 - 11:37 pm:
You can expect probably just one broad peak because keto-enol tautomery occurs while the compound travels down the gc column with the keto form having a sligtly different retention time than the enol isomer.
By ori951 on Tuesday, May 25, 2004 - 12:37 am:
thanks a lot for your information.
By Shalash on Tuesday, May 25, 2004 - 03:52 am:
hi
im trying to devolopment anew method for Napeoxen Na to determine the Residual solvent (Toluene,Acetone and Dichloromethane)by using water as solvent
when i development asuitable method (using DB-Wax as Capillary column )but when bgain amethod validation, i faced aproblem for atoluene (Recovery falid)
could any one help me
thanks alot
shalash
By Anonymous on Thursday, May 27, 2004 - 10:49 am:
You may want to look at the source of your water. Here we use Pharmaceutical grade water and always get a toluene peak, even in the blank. When we purchased Ultra Grade water from Fisher (or any other vendor), this was not as much of a problem. Am I correct in assuming that your recoveries are high? If so, then it is because of the Toluene present in the water. This is a problem in a lot of places.
By G. Sartor on Saturday, May 29, 2004 - 02:02 pm:
Regarding the toluene-problem: if your recovery is too low, try to disolve the standards in DMSO and dilute with water to the working concentration.