There are black spots in my column.Is it a sign of degaradation?
Thank you for replying me
By Jason Ellis on Tuesday, July 13, 2004 - 08:07 am:
Usually, that is a sign of contamination of the column by sample residues.
By GC Dave on Tuesday, July 13, 2004 - 08:50 am:
I have had a similar problem with a supposed high temperature phase in the past immediately after I had conditioned it. The column was unusable (no retention).
I thought the spots might be cooked up stationary phase.