HPLC-MS of CoA-Esters and Nucleotides (ATP,AMP,ADP)

Chromatography Forum: LC-MS & GC-MS Archives: HPLC-MS of CoA-Esters and Nucleotides (ATP,AMP,ADP)
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Friday, February 16, 2001 - 12:39 pm:

Do somebody have experience with HPLC-MS of CoA-esters and Nucleotides (AMP,ADP,ATP)on RP18 columns (especially with ion pairing agents).
I tried 1mM Ammoniumformiate at pH 7.3 in ESI neg and its working not to bad, but sometimes I need a little bit more retention.

best regards, thanks for help


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