By Jacob on Wednesday, April 11, 2001 - 06:45 am:
The manual of our fluorescence detector (Jasco
821-FP) states a signal-to-noise-ratio S/N = 120
or greater for the Raman peak of water (excitation
wavelength 350 nm). Can anyone tell me what the
Raman peak of water is, how it can be measured and
thus how the stated specification can be verified?
By H W Mueller on Tuesday, April 17, 2001 - 12:04 am:
Raman is a form of light scattering which involves a change of wave length (energy) of the scattered light. For details you will have to go to the library....
You should see the Raman band by running a fluorescence spec. on highly purified water (should have absolutely no fluorescence!), starting, as an example, on the upper part of the Rayleigh scattering band (light scattered at the ex. band, 350 nm), going to longer wave length (should be near 400 nm). You can distinguish the Raman band from fluorescence by varying the ex. wave length. Raman changes the same way, fluorescence does not (regarding wave length, not intensity).
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