By Pat Hawthorne on Thursday, November 1, 2001 - 12:46 am:
Hi
Im looking for a HPLC or LC-MS method to separate chlorotetracycline and it's 4-epimer isomer.
Any suggestions would be welcome
By Anonymous on Thursday, November 1, 2001 - 09:57 am:
I have done it with a 100X4.6 mm C8 column and a 10 mM ammonium acetate/0.5% acetic acid buffered H2O/AcN gradient. The peaks came out sharper than with a C18 column. Several different solvent ratios worked, but I had to buffer the AcN as well as the water to get reproducible results. I used LC/MS analysis.
By Anonymous on Wednesday, November 7, 2001 - 04:26 pm:
I use 150x4.6mm PLRP-S column with oxalic acid/AcCN mobile phase. The peaks of chlortetracycline and its 4-epimer are well separated.