By Anonymous on Monday, May 27, 2002 - 05:05 am:
I am about to start global protein profiling by MS (proteomic analysis) after clean-up/enrichment and groping by SPE, i.e. non-charged proteins by reversed-phase SPE, positively-charged proteins by cation-exchange SPE and negatively-charged proteins by anion-exchange SPE.
Any previous experiences, please advise.
I don't need to separate all proteins like in most of the LC-MS works. Instead I need good SPE cartridges to retain certain groups of proteins for later elution followed by MS analysis. I believe the important parameters will be: sample pH, SPE functional group and pore/particle size, rinsing buffer and eluents. The selection of SPE cartridges is the key.
By Anonymous on Monday, May 27, 2002 - 06:38 am:
I am curious if there are other online discussion forums related to proteomics, especially to LC-MS of proteins.
By mkane on Monday, May 27, 2002 - 07:37 am:
I'm not personally experienced in this but as they say "I know a man who is" and I've asked him the question. From what you've described as a method development strategy you could try a multi-mode cartridge from IST. It contains C18, strong cation exchanger and a strong anion exchanger all in the one cartridge. I've seen somthing about the separation of acid basic and neutrals on a cation and hydrophobic column as well.
I agree that pH and pore size will be critial. Have you thought of using a array to do method development.
By mkane on Monday, May 27, 2002 - 07:49 am:
See following list for some clues.
Base Peak Mass Spectrometry Resource (Hosted by SpectroscopyNow)
http://www.spectroscopynow.com/Spy/ms/
Cheatham's Instrumentation Pages
http://www.geochemistry.syr.edu/cheatham/
I-Mass
http://www.i-mass.com/
IonSource
http://www.ionsource.com/
International Mass Spectrometry
http://www.i-mass.com/
MS Blog
http://ch335c.chem.lsu.edu/resources/ms/
MS Links
http://www.mslinks.com/
Proteomics Surf
http://proteomicssurf.com/
The Yahoo Index, Spectroscopy Page
http://dir.yahoo.com/science/chemistry/
American Chemical Society Division of Analytical Chemistry
http://www.acs-analytical.duq.edu/
The American Society for Mass Spectrometry
http://www.asms.org/
Australian and New Zealand Society for Mass Spectrometry
http://www.latrobe.edu.au/www/anzsms/
Belgian Society for Mass Spectrometry
http://masseroute.cico.ucl.ac.be/bsms/bsms.htm
British Mass Spectrometry Society
http://www.bmss.org.uk/
Canadian Society for Mass Spectrometry
http://www.csms.inter.ab.ca/
Chinese-American Society for Mass Spectrometry
http://www.geocities.com/CapeCanaveral/Lab/7486/massspec.html
Danish society for Mass Spectrometry
http://www.dsms.dk/
Dutch Society for Mass Spectrometry
http://www.denvms.nl/
European Society for Mass Spectrometry
http://www.bmb.leeds.ac.uk/esms/
French Society of Mass Spectrometry
http://wwwadm.admp6.jussieu.fr/sfsm/
International Mass Spectrometry Society
http://www.imss.nl/
Irish Mass Spectrometry Society
http://homepages.iol.ie/~instchem/imss98/imss.htm
Mass Spectrometry Society of Japan
http://wwwsoc.nii.ac.jp/mass/
Norwegian Society for Mass Spectrometry
http://www.chem.uit.no/nsms/
Polish Mass Spectrometry Society
http://ptsm.ichf.edu.pl/ptsm_main.htm
Society for Applied Spectroscopy
http://www.s-a-s.org/
South African Association for Mass Spectrometry
http://www.up.ac.za/academic/acadorgs/saams/home.html
Spanish Society for Mass Spectrometry
http://www.iibb.csic.es/seem/
Swedish Mass Spectrometry Club
http://www.chemsoc.se/sidor/Sekt/spektro.htm
Swiss Group for Mass Spectrometry
http://www.sgms.ch/