Buffer pH 7.4 for LC/MS
Chromatography Forum: LC-MS & GC-MS Archives: Buffer pH 7.4 for LC/MS
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Jean-Philippe Lambert on Thursday, June 27, 2002 - 11:44 am:

Hi guys,

I need a buffer a pH 7.4 which is MS friendly for a new assay I'm developing. Any suggestions will be welcome.

Thanks for your time.

Jean-Philippe

Top of pagePrevious messageNext messageBottom of pageLink to this message  By Uwe Neue on Thursday, June 27, 2002 - 03:08 pm:

There are indications in the literature that ammonium phosphate is reasonably MS compatible. We have also been partially successful with ammonium bicarbonate acidified to the desired pH with an MS compatible acid such as formic acid. However, you should check the pH after degassing and after some period of time sitting around. Colleagues warned me of slow changes in the pH, possibly due to evaporation of carbon dioxide.

Top of pagePrevious messageNext messageBottom of pageLink to this message  By Jean-Philippe Lambert on Friday, June 28, 2002 - 11:46 am:

I tried ammonium bicarbonate and it worked like a charm (well good enough for this stage of development).


Thanks a lot Uwe

Jean-Philippe

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