By Geoffrey Kite on Wednesday, August 18, 1999 - 03:31 am:
Does anyone know of a MS compatible LC method for underivatised amino acids? I actually want to analyse non-protein amino acid analogues from plants so would like molecular weight information on the underivatised molecule to aid identification of unknowns. Thanks.
By Mike on Wednesday, August 18, 1999 - 09:02 am:
As a starting point, check out the July issue of LCGC. pp. 626-632. Some interesting pointers. Additionally these guys were also from the UK (Astra-Zeneca Pharmaceuticals, Cheshire).
By benjamin esquivel on Tuesday, August 31, 1999 - 08:23 am:
Dionex has developed a good ion exchange system to separate amino acids. The detection system they employ is electrochemical.
As it is, the mobile phase employed is sodium acetate/sodium hydroxide/water. It is possible that you could change these to ammonium-based salts and therefore make it compatible with LC-MS.
Give Dionex a call.
By Anonymous on Monday, October 4, 1999 - 12:08 am:
please,give me more information about gas-liquid-cromatography
By Anonymous on Thursday, October 7, 1999 - 01:26 pm:
Can't do underivatized AA's by GC; they're thermally unstable. They *can* be derivatized, however. Check out http://www.registech.com/derivatization/
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