Hi!
Any body has a LC-MS method for alkyl polyglycosides?
My reaction mixture will have lauryl alcohol, glucose, and alkyl mono-, di-, tri , polyglycosides. I am developing a method using acetonitrile / water mobile phase, APCI +ve polarity; 0.1% acetic acid solution as make up flow. I guess this is not suitable to detect glucose in the mixture. I saw one method for glucose using APCI -ve polarity, using Acetonitrile / CHCl3 post-column to get chloride ions.
Can anyone suggest a method which can be used to quantify each of these components?
Thanks
Syamala

I've never analyzed sugars, but have found APCI negative most suitable for neutral alcohols (i.e. compounds similar to lauryl alcohol). Here's a reference that might be helpful for the sugars etc:

Analysis of Highly Polar Compounds of Plant Origin: Combination of Hydrophilic Interaction Chromatography and Electrospray Ion Trap Mass Spectrometry, Analytical Biochemistry, Volume 301, Issue 2, 15 February 2002, Pages 298-307

In summary, sugars give NH4+ or Na+ adducts in positive, no MH+. In negative they give (M-H)- and/or (M+acetate)-. Chromatography is HILIC. Not sure if lauryl alcohol would be retained in HILIC, however.

One argument against using chloride adducts is that you split your signal two ways. Also, you may not need the acid post-column in APCI. If you've done the experiment and the acid helps, I'd love to hear about it here. Good luck.

Yes, HILIC seems to be an suitable alternative. Often people report on improved MS sensitivity when HILIC is used for the separation.

Don't know much about MS detection, but from a chromatography standpoint you are likely to be equally successful with RP as with HILIC. In case of question, I would go for RP first. You underivatized materials will run through unretained, and the lauryl derivatized glycosides will be well retained.