By Anonymous on Tuesday, November 23, 1999 - 08:04 am:
I need to analyze some basic drugs using LCMS. The current UV method mobile phase contains 0.4% octane sulfonic acid (Na salt), THF, and propanol. Can anyone recommend volatile ion pairing reagents, besides HFBA, that can be used for LCMS analyses? TIA.
By Uwe Neue on Sunday, December 19, 1999 - 07:51 pm:
No need to use ion pairing. Use a high-quality modern HPLC column with a gradient from 5% to 95% acetonitrile with 0.1% formic acid. If you contact me at Uwe.Neue@Prodigy.com, I'll give you advice on the best columns.
Best regards
Uwe
By Grey Havner on Tuesday, December 21, 1999 - 05:51 am:
Ammonium acetate is a very common LCMS buffer, and it does have some ion-pairing effect.
Grey Havner
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