By bill tindall on Thursday, July 1, 1999 - 12:32 pm:
we want to develop a chromatography demo to take to schools where we would analyze different kinds of soda for the permutations of aspartame and caffeine. Can anyone suggest a column and conditions for a robust, fast, isocratic separation? Any other ideas for good school demos?
By Anonymous on Thursday, July 1, 1999 - 02:35 pm:
I'm not sure about the aspartame (as a dipeptide, it may be more touchy), but just analyzing for caffeine is a piece of cake. Most any C18 column should work using MeOH/water and UV detection around 250 nm. You'll have to tweak the % MeOH for a given type of column.
You might also do a literature search in J. Chem. Ed. I'm sure they have plenty of examples.
Good luck!
By Anonymous on Friday, July 2, 1999 - 05:06 am:
Try this link:
http://www.waters.com:8080/WatProd.nsf/docs/981281.html
It discribes the exact demo you are looking for.