By penguin2C on Monday, October 18, 1999 - 02:22 pm:
I am a 10th grade student and I have a chromatography lab and some of
the ?'s on the lab are as follows:
1)no solutes are colored-how analyze the solution
2)solution is very dilute w/solutes to be separated-how do i complete
the analysis
3)no solutes separate from solution-how do I analyze still using paper
chromatography. (I think it has to do with changing the paper or liquid
but I am not sure)
If you can help me using relatively understandable vocabulary it would
be much appreciated
By Anonymous on Monday, October 18, 1999 - 10:48 pm:
What about:
1) visualize the spots with any of the following: a) UV light (most organic compounds absorb UV light) b) spraying the developed paper chromatogram with a reagent that reacts with the substances separated to give colored spots (see for instance the discussion titled "it has to be explained..." above)
2) either concentrate the solution or apply more solution to the paper
3) 2 possibilities: a) there is just 1 solute in the solution (teachers sometimes make dirty tricks to students...) or b) change the composition of the solvent used to develop your paper chromatogram (type of solvent, ratio of solvent etc.)
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