Training: Advanced HPLC Method Development

Who should take this course?
If you've ever wished that HPLC method development were more logical and methodical, this is the course for you. Attendees should have at least one year of HPLC experience in the laboratory and at least some involvement in developing new methods or troubleshooting older ones. This course is for chromatographers who want to develop better methods faster and less expensively.

What does it cover?
In this two-day workshop, you will learn a powerful new approach to systematic method development. Learn the modern approach to method development:

• Define your objectives clearly. For example, your objective may be:
  - to separate and account for all impurities at the 0.1% level
  - to develop rugged, precise methods quickly and efficiently
  - to have validated methods accepted by the FDA
• Apply the best approach to separating mixtures that contain acids, bases, or isomers -- or to separating complex samples.
• Take advantage of recent improvements in column technology to assist you in your method development efforts.

Learn how to develop a satisfactory method:

• Learn to develop rugged methods that have superior separation repeatability
• Design system suitability checks and validation into your method
• Understand the factors that control precision.

Improve your troubleshooting skills

• Develop improved problem-solving techniques based on this new approach to method development.

Improve your understanding of the effects of chromatographic variables through the use of computer modeling

• Solve six case study problems that clearly demonstrate how this methodology works.
• Apply your new knowledge to your own and other students' actual data during in-class problem-solving sessions.

What will I get from this course?
Using chromatographic computer modeling, you will solve a series of real-world problems that clearly demonstrate how this approach to method development works. The principles used in computer modeling are fully applicable to separations in your lab. You will learn how to do the best possible chromatography. Save hours of frustration and improve your results time after time.

$1275 (Regular)		-- Select Course Date/Location -- October 15-16, 2007, Research Triangle Park, NC February 12-28, 2008, Live on the Web! March 31-April 1, 2008, Somerset, NJ April 21-22, 2008, Rosemont, IL (Chicago area) June 2-3, 2008, Foster City, CA (San Francisco area) September 9-25, 2008, Live on the Web October 6-7, 2008, Saddle Brook, NJ December 1-2, 2008, San Diego, CA
$1175 (30-Day Advance)		-- Select Course Date/Location -- February 12-28, 2008, Live on the Web! March 31-April 1, 2008, Somerset, NJ April 21-22, 2008, Rosemont, IL (Chicago area) June 2-3, 2008, Foster City, CA (San Francisco area) September 9-25, 2008, Live on the Web October 6-7, 2008, Saddle Brook, NJ December 1-2, 2008, San Diego, CA

Schedule for this course

Course Outline
Day 1
Registration/continental breakfast
Introduction
Introduction HPLC Method Development
Review of Separation Basics
Workshop: Varying %B and Column Conditions
Lunch
New Column Developments
Reversed-Phase HPLC for Neutral Samples
Workshop: Effects of Solvent Type on Separation
Workshop: Mixing Solvents
Reversed-Phase HPLC of Ionic Samples
Workshop: Optimizing pH and %B for a Mixture of Acids and Bases

Day 2
Continental breakfast
Ion-Pair HPLC
Ion-Pair HPLC continued
Workshop: Ion-Pair Chromatography
Gradient Elution
Workshop: Gradient Elution
Lunch
Experimental Aspects of Gradient Elution
Dealing with Special Samples
Improving Precision, Method Validation, System Suitability
Discussion of Student Problems