Training: HPLC
Basics, Equipment, and Troubleshooting
Who
should take this course?
If you use chromatography on the job and would
like a better understanding of how it works,
"HPLC Basics, Equipment, and Troubleshooting"
is the course for you. It's designed for chemists
and biochemists who use HPLC as a regular part of
their jobs, but technicians with some HPLC
experience will also find the course valuable. No
previous HPLC training is assumed; however, much
of the course will appeal to the experienced
chemists who want a firm grounding in the basics
of HPLC.
What
does it cover?
"The Practice of Modern HPLC" explains
chromatography in practical terms from the ground
up. Here's what you'll learn in this three-day
course:
- The
basic principles of HPLC
- The
five fundamental HPLC modes
- The
easy and logical way to adjust
experimental variables to get a good
separation
- The
best approach to use for various
chromatographic applications
- How
to maximize column life
- Special
techniques such as gradient elution,
quantitation, and sample pretreatment
- The
operating principles of each module in an
HPLC system
- Proven
techniques for systematic problem-solving
and instrument maintenance
- The
most effective, timesaving, money saving
approaches to preventing common hardware
problems and method failures.
- And
much, much more
What
will I get from this course?
You will acquire a good understanding of the
fundamentals of chromatography and learn simple
tips and guidelines to make your chromatography
work easier and more efficient. What
you learn will demystify your instrument. You'll
find that all of the perplexing and frustrating
problems your experience have simple and logical
solutions. And, better yet, you'll learn that
most of these problems are preventable. The
techniques you learn from this course will make
you more effective at work and save you big
headaches! If you've
never taken a formal course or if you need a
refresher, it's time to learn HPLC the right way.
Students tell
us that this "extremely practical course"
is a must for everyone who uses HPLC.
How
much does it cost ?
The standard
course fee is $1297/person. This includes the
course, notes, continental breakfasts and lunches.
"Live on the Web" course fee is $997/person.
This includes the course and a download of the
course notes.
Additional
registrants from the same company are half-price.
Call us at (800) 379-5221 to arrange multiple-registrant
pricing
Register On-Line Now!
Schedule
for this course
Course
Outline
Day 1
Registration/continental breakfast
HPLC Basics
Mobile Phase Reservoirs
Pumps and Mixing
Tubing and Fittings
Lunch
Sample Injectors & Autosamplers
HPLC Detectors
Conclusions/Questions
Day 2
Continental breakfast
HPLC Columns
Reversed-Phase HPLC
pH Effects and Ion-Pair
Lunch
Gradient Elution
Column Problems and Fixes
Tailing Bands
Quantitative HPLC
Conclusions/Questions
Day 3
Continental breakfast
Separation Chemistry Problems
Sample Preparation
Ion-Exchange HPLC
Normal-Phase HPLC
Size-Exclusion HPLC
Lunch
Bioseparations by HPLC
Case Studies
Review
Conclusion
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