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Training:
HPLC Basics, Equipment, and
Troubleshooting
Who should take this course?
If you use chromatography on the job and would like a better
understanding of how
it works, "HPLC Basics, Equipment, and Troubleshooting" is the course
for you. It's
designed for chemists and biochemists who use HPLC as a regular part of
their jobs, but technicians with some HPLC experience will also find
the course valuable. No previous HPLC training is assumed; however,
much of the course will appeal to the experienced chemists who want a
firm grounding in the basics of HPLC.
What does it cover?
"The Practice of Modern HPLC" explains chromatography in
practical terms from the ground up. Here's what you'll learn in this
two-day course:
- The basic principles of
HPLC
- The five fundamental HPLC
modes
- The easy and logical way
to adjust experimental variables to get a good separation
- The best approach to use
for various chromatographic applications
- How to maximize column
life
- Special techniques such
as gradient elution, quantitation, and sample pretreatment
- The
operating principles of each module in an HPLC system
- Proven
techniques for systematic problem-solving and instrument maintenance
- The
most effective, timesaving, money saving approaches to preventing
common hardware problems and method failures.
- And much, much more
What will I get from this course?
You will acquire a good understanding of the fundamentals of
chromatography and learn simple tips and guidelines to make your
chromatography work easier and more efficient. What you learn will
demystify your instrument.
You'll find that all of the perplexing and frustrating problems your
experience have simple and logical solutions. And, better yet, you'll
learn that most of these problems are preventable. The techniques you
learn from this course will make you more effective at work and save
you big headaches! If you've never taken a
formal course or if you need a refresher, it's time to learn HPLC the
right way. Students
tell us that this "extremely practical
course" is a must for everyone who uses HPLC.
Schedule
for this course
Course Outline
Day 1
Registration/continental breakfast
HPLC Basics
Mobile Phase Reservoirs
Pumps and Mixing
Tubing and Fittings
Lunch
Sample Injectors & Autosamplers
HPLC Detectors
Conclusions/Questions
Day 2
Continental breakfast
HPLC Columns
Reversed-Phase HPLC
pH Effects and Ion-Pair
Lunch
Gradient Elution
Column Problems and Fixes
Tailing Bands
Quantitative HPLC
Conclusions/Questions
Day 3
Continental breakfast
Separation Chemistry
Problems
Sample Preparation
Ion-Exchange HPLC
Normal-Phase HPLC
Size-Exclusion HPLC
Lunch
Bioseparations by HPLC
Case Studies
Review
Conclusion
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