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Lab Tech


Training: HPLC Basics, Equipment, and Troubleshooting

Who should take this course?
If you use chromatography on the job and would like a better understanding of how it works, "HPLC Basics, Equipment, and Troubleshooting" is the course for you. It's designed for chemists and biochemists who use HPLC as a regular part of their jobs, but technicians with some HPLC experience will also find the course valuable. No previous HPLC training is assumed; however, much of the course will appeal to the experienced chemists who want a firm grounding in the basics of HPLC.

What does it cover?
"The Practice of Modern HPLC" explains chromatography in practical terms from the ground up. Here's what you'll learn in this two-day course:

  • The basic principles of HPLC
  • The five fundamental HPLC modes
  • The easy and logical way to adjust experimental variables to get a good separation
  • The best approach to use for various chromatographic applications
  • How to maximize column life
  • Special techniques such as gradient elution, quantitation, and sample pretreatment
  • The operating principles of each module in an HPLC system
  • Proven techniques for systematic problem-solving and instrument maintenance
  • The most effective, timesaving, money saving approaches to preventing common hardware problems and method failures.
  • And much, much more

What will I get from this course?
You will acquire a good understanding of the fundamentals of chromatography and learn simple tips and guidelines to make your chromatography work easier and more efficient.
What you learn will demystify your instrument. You'll find that all of the perplexing and frustrating problems your experience have simple and logical solutions. And, better yet, you'll learn that most of these problems are preventable. The techniques you learn from this course will make you more effective at work and save you big headaches! If you've never taken a formal course or if you need a refresher, it's time to learn HPLC the right way. Students tell us that this "extremely practical course" is a must for everyone who uses HPLC.

$1275 (Regular)     
$1175 (30-Day Advance)     

Schedule for this course

Course Outline
Day 1
Registration/continental breakfast
HPLC Basics
Mobile Phase Reservoirs
Pumps and Mixing
Tubing and Fittings
Lunch
Sample Injectors & Autosamplers
HPLC Detectors

Conclusions/Questions

Day 2
Continental breakfast
HPLC Columns
Reversed-Phase HPLC
pH Effects and Ion-Pair

Lunch
Gradient Elution
Column Problems and Fixes
Tailing Bands
Quantitative HPLC
Conclusions/Questions

Day 3
Continental breakfast
Separation Chemistry Problems
Sample Preparation
Ion-Exchange HPLC

Normal-Phase HPLC
Size-Exclusion HPLC
Lunch
Bioseparations by HPLC
Case Studies
Review
Conclusion

 

 
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