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Training:
Practical Separations of
Biopharmaceuticals
Who
should take this course?
If you are a biochemist or biologist with limited experience using
HPLC, this course will acquaint you with instrumental techniques for
analysis and lab-scale purification of biomolecules. If you are an
analytical chemist with experience in the use of HPLC separations for
small molecules, this course will help you apply these techniques to
peptides, proteins and nucleic acids.
What
does it cover?
The focus of this two-day course is on the practical aspects of using
high performance separation techniques for biomolecules, with an
emphasis on proteins and peptides. In this course you will learn:
- Fundamentals
of HPLC mechanisms of band broadening for large molecules
- An
overview of HPLC instrumentation with an emphasis on preventive
maintenance and troubleshooting
- Column
and mobile phase selection for reversed-phase, ion-exchange and
size-exclusion chromatography
- Strategies
for developing and optimizing HPLC methods
- HPLC
column maintenance and troubleshooting
What
will I get from this course?
You will learn how to chose the appropriate separation technique for
your application and how to select the best chromatographic or
electrophoretic separation mode to meet your separation goals. You will
learn how to develop and optimize an LC method and how to troubleshoot
it when problems occur. You will learn how to handle columns to achieve
good performance and satisfactory lifetime. The focus of this course
will be on practical guides and tips to achieve successful biomolecule
separations.
You
will apply the concepts presented in this course by using computer
modeling to optimize gradient separations of proteins and peptides. The
principles demonstrated by computer simulations are fully applicable to
separations in your lab.
This
course is taught as an in-house course
only. To
discuss in-house course options, call (800) 379-5221 or send us an e-mail with a brief
description of your training needs, and we'll call you with details.
Course
Outline
Day
1
Introduction
Review
of Chromatographic Theory and Contributions to Band Broadening
Selecting and Configuring HPLC Instrumentation
Overview of HPLC Column Packings
Gradient Elution
Size-Exclusion HPLC
Reversed-Phase HPLC
Conclusion
Day
2
Ion-Exchange HPLC
Special Techniques (High Performance Affinity Chromatography and
Related Techniques)
Computer Workshop: Gradient Optimization in Reversed-Phase and
Ion-Exchange Chromatography
Column Maintenance and Troubleshooting
Conclusion
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