|
Training:
Bioanalytical LC-MS
Who
should take this course?
This course is designed to provide basic knowledge and practical
applications of LC-MS to the analysis of drugs in metabolites in
biological extracts. Although no previous experience with HPLC is
necessary, the course will be especially valuable to those with some
chromatography experience.
What
does it cover?
"Practical LC-MS for Chromatographersä is a two-day course
explaining the principles of mass spectrometric detection, the design
and operation of mass spectrometers, and the interfacing of the MS to
the liquid chromatograph to obtain both qualitative and quantitative
data. This two-day course covers:
- MS
operation, including the operation of the most popular LC-MS interfaces
- How
a quadrupole mass filter works
- MS
calibration and optimization
- Ion
production, fragmentation and detection
- Operation
in MS, MS-MS and MS-MS-MS modes
- Structure
determination by product ion analysis
- Quantitative
analysis
- Method
development and validation
- Techniques
to get the most from your LC-MS
What
will I get from this course?
You will learn how a mass spectrometer works, the advantages (and
limitations) of adding mass spectrometric detection to your liquid
chromatographic analyses, and how to use MS to approach separation and
detection problems intractable by other methods. By understanding the
central roles of the LC-MS interface and the molecular ion, you will
have a solid grounding on which to further build your LC-MS expertise.
This
course is taught as an in-house course
only. To
discuss in-house course options, call (800) 379-5221 or send us an e-mail with a brief
description of your training needs, and we'll call you with details.
Course
Outline
Day
1
Registration/continental breakfast
Introduction
Quadrupole Physics
Basics of Quadrupole Instrumentation
Lunch
The Molecular Ion
Calibration and Tubing
Conclusion
Day
2
Continental breakfast
Qualitative Analysis
Quantitative Analysis
Lunch
Maintenance and Repair
Regulatory Considerations
Case Studies
Conclusion
|
