Training: The Practice of Modern HPLC

Who should take this course?
If you use chromatography on the job, but don't really understand how it works, "The Practice of Modern HPLC" is the course for you. It's designed for chemists and biochemists who use HPLC as a regular part of their jobs, but technicians with some HPLC experience will also find the course valuable. No previous HPLC training is assumed; however, much of the course will appeal to the experienced chemists who want a firm grounding in the basics of HPLC.

What does it cover?
"The Practice of Modern HPLC" explains chromatography theory in practical terms from the ground up. Here's what you'll learn in this two-day course:

• The basic principles of HPLC
• The components of an HPLC system
• The five fundamental HPLC modes
• The easy and logical way to adjust experimental variables to get a good separation
• The best approach to use for various chromatographic applications
• How to maximize column life
• Special techniques such as gradient elution, quantitation, and sample pretreatment
• And much, much more

What will I get from this course?
You will acquire a good understanding of the fundamentals of chromatography and learn simple tips and guidelines to make your chromatography work easier and more efficient. If you've never taken a formal course or if you need a refresher, it's time to learn HPLC the right way. Most students wish they had taken a course like this years ago.

This course is taught as an in-house course only. To discuss in-house course options, call (800) 379-5221 or send us an e-mail with a brief description of your training needs, and we'll call you with details.

Course Outline

Day 1
HPLC Basics
HPLC Equipment
HPLC Equipment concludes
HPLC Columns
Reversed-Phase HPLC
pH Effects and Ion-Pair
Conclusion

Day 2
Gradient Elution
Tailing Bands
Quantitative HPLC
Sample Preparation
Ion-Exchange HPLC
Normal-Phase HPLC
Size-Exclusion HPLC
Bioseparations by HPLC
Review
Conclusion