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Training:
The Practice of Modern HPLC
Who should take this course?
If you use chromatography on the job, but don't really understand how
it works, "The Practice of Modern HPLC" is the course for you. It's
designed for chemists and biochemists who use HPLC as a regular part of
their jobs, but technicians with some HPLC experience will also find
the course valuable. No previous HPLC training is assumed; however,
much of the course will appeal to the experienced chemists who want a
firm grounding in the basics of HPLC.
What does it cover?
"The Practice of Modern HPLC" explains chromatography theory in
practical terms from the ground up. Here's what you'll learn in this
two-day course:
- The basic principles of
HPLC
- The components of an HPLC
system
- The five fundamental HPLC
modes
- The easy and logical way
to adjust experimental variables to get a good separation
- The best approach to use
for various chromatographic applications
- How to maximize column
life
- Special techniques such
as gradient elution, quantitation, and sample pretreatment
- And much, much more
What will I get from this course?
You will acquire a good understanding of the fundamentals of
chromatography and learn simple tips and guidelines to make your
chromatography work easier and more efficient. If you've never taken a
formal course or if you need a refresher, it's time to learn HPLC the
right way. Most students wish they had taken a course like this years
ago.
This
course is taught as an in-house course
only. To
discuss in-house course options, call (800) 379-5221 or send us an e-mail with a brief
description of your training needs, and we'll call you with details.
Course Outline
Day 1
Registration/continental breakfast
HPLC Basics
HPLC Equipment
Lunch
HPLC Equipment concludes
HPLC Columns
Reversed-Phase HPLC
pH Effects and Ion-Pair
Conclusion
Day 2
Continental breakfast
Gradient Elution
Tailing Bands
Quantitative HPLC
Sample Preparation
Ion-Exchange HPLC
Lunch
Normal-Phase HPLC
Size-Exclusion HPLC
Bioseparations by HPLC
Review
Conclusion
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