Getting Started in HPLC

Contents

• Introduction
• 1. The Basics
  • A. What is Chromatography?
  • B. The Chromatography "Family Tree"
  • C. HPLC Instruments
  • D. Chromatography by the Numbers
• 2. LC Equipment
  • A. Solvent Reservoirs
  • B. HPLC Pumps
  • C. Injectors
  • D. Columns
  • E. Detectors
  • F. Data Systems
  • G. Tubing and Fittings
• 3. Getting the Separation
  • A. Column Chemistry
  • B. Mobile Phase Chemistry
  • C. Gradient HPLC
  • D. Other Factors
• 4. Using the Chromatogram to Get Results
  • A. Qualitatative Analysis
  • B. Units and Concentration
  • C. Volumetric Dilution
  • D. Precision and Accuracy
  • E. Calibration
  • F. Putting it All Together
• 5. Final Exam
• Glossary: The Language of HPLC
• Recommended Reading
• Links to Additional Information

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