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| If you are currently using
HPLC in your work, then you are already familiar with a
chromatogram like the one shown at the right. This
chromatogram has 6 peaks or bands, indicating that there
were at least 6 chemical compounds present in the sample.
You probably know that you can identify the compounds by
the position of their peaks in the chromatogram, and that
you can estimate the quantity of each compound in the
sample from the size of the corresponding peak.
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| What you may
not already know is how the chromatogram is generated and
why we can use it for both qualitative and quantitative
analysis.
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In this
section, we will:
- describe the basic
mechanism common to all forms of chromatography
- discuss the
relationship between HPLC and other forms of
chromatography
- describe the basic
components or modules common to all HPLC
instruments
- discuss the
measurements we can make on a chromatogram that
let us describe our results.
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