Getting Started in HPLC

Section 3. Getting the Separation

In this section, we will discuss one form of HPLC called "reversed-phase chromatography". This is the most commonly used type of HPLC separation.

Reversed-phase HPLC methods use a special kind of column and a mobile phase composed of a mixture of water (or an aqueous buffer) and an organic solvent such as acetonitrile or methanol. Later on, we will talk about other LC techniques such as ion-pair chromatography, normal-phase chromatography, ion-exchange chromatography, and size-exclusion chromatography.



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Last revised: April 02, 2001.