| This unit
begins with the chromatogram we get after we have run a
sample. At this point we have one or more peaks, which
tells us something about how many compounds are present
in the sample.
|
||||
| In the example at the
right, we see three peaks, each corresponding to a
different compound. So there are at least three compounds
present in this sample. But what we usually want to know
is what compounds are present in the
sample and how much of each compound is
present. The technique of "qualitative analysis" means finding out the
identity of different compounds in the sample. "quantitative analysis" refers to measuring the
concentration or amount of each compound in the sample. Usually we want to carry out quantitative analysis on the sample, but this also means identifying the compounds whose concentrations we measure. In LC we use retention times to establish peak identity and band size (peak height or area) to measure concentration. In this example we have a report with the values of retention time, peak area, and peak height below the chromatogram. |
|
|||
