By Anonymous on Tuesday, August 1, 2000 - 09:49 am:
can anyone tell me why acidic substances are usually separated by thin layer chromatography whilst basic substances are mainly separated by gas chromatography. Thanks
By H W Mueller on Tuesday, August 8, 2000 - 03:34 am:
How about hydrogen bonding by the carboxl group which lowers volatility? However, in about the last 20 years, when I did perform GC it was for acids (methyl esters of fatty acids).
Also, underivatized acids have a tendency to decarboxylate at elevated temps, some can apparently survive GC, though.
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