LC Archives

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Chromatography Forum: LC Archives

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Welcome to LC Archives. You may read any of the discussions by clicking on the appropriate link. To start a new discussion of your own, go to the LC Message Board.

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• Combinatorial Chemistry May 3 - 01:47 pm
• Late Elution April 30 - 02:53 pm
• Washing column with 100% aqueous. July 23 - 08:30 am
• Chiral Chromatography July 23 - 08:34 am
• Blown up detector cell in HP DAD May 24 - 04:07 pm
• Need Better Resolution May 20 - 11:20 am
• Variable Retention Time in Gradient HPLC of Protein May 18 - 10:06 pm
• Analysis of GMP, GDP and GTP May 19 - 09:10 am
• Can I detect this by UV? May 20 - 10:13 am
• What is an appropriate internal standard for lactate/pyruvate analyses of brain microdialysates? May 22 - 02:01 pm
• Analytical Chemistry Laboratory Layout May 25 - 03:57 pm
• 0,001M H2SO4 as mobille phase May 27 - 12:10 pm
• Resin-Based Column July 13 - 10:59 am
• Gradient Runs hampered by Water Purity? July 23 - 04:01 am
• Quantitation June 10 - 08:25 am
• Contamination peaks in TFA and ACN gradient June 16 - 10:05 am
• Reporting zero June 11 - 04:51 am
• Ion-pairing reagent June 13 - 11:47 pm
• HP 1090L HPLC May 31 - 06:18 am
• Ultrahigh pressure liquid chromatography June 1 - 12:57 pm
• Dextrose Interfering With Lactic Acid HPLC Assay September 22 - 11:05 am
• USP columns June 11 - 06:37 am
• Method validation June 1 - 07:29 am
• PL GEL COLUMS VS PHENOGEL COLUMNS : May 24 - 03:25 pm
• Reverse Phase, Large Scale Liquid Column Preparative Separation to Precede HPLC June 9 - 11:23 am
• Logbook June 17 - 08:03 am
• Direct injection of invitro metabolism samples June 15 - 06:08 am
• Quantifying triethylphosphate (TEP) June 8 - 02:47 pm
• Method validation June 28 - 08:31 am
• UV flow cell cleaning (?) June 11 - 10:38 pm
• Anybody make an HPLC column packed with alumina? July 29 - 05:22 am
• Second column confirmation June 11 - 03:20 pm
• Solvent Filtration June 25 - 03:27 am
• DNA with HPLC July 3 - 01:58 am
• Cyano Columns for RP-HPLC June 23 - 12:49 pm
• HIC problems June 10 - 11:50 am
• HAUNTING GHOST PEAKS June 18 - 03:55 pm
• Auto-injector plugged with septum cores, Help? June 23 - 09:41 am
• Gradient Polymer Elution Chromatography June 13 - 09:11 am
• HPLC Equipment Qualification June 25 - 02:46 pm
• A smile face gradient baseline June 18 - 03:38 pm
• Expiration dating June 23 - 08:50 am
• Problems with base noise in the begining of all chromatograms June 23 - 02:34 am
• Alternatives to a normal phase separation? June 19 - 02:14 am
• Negative Spikes in Sample Peak June 19 - 02:24 am
• Self-Service ? October 21 - 07:38 pm
• Side bar to Self-Service topic June 29 - 11:26 am
• LC Anion Columns June 28 - 11:28 am
• Peak shape depends on the wavelength? June 30 - 10:10 am
• Glucoseamine by hplc using uv June 26 - 09:44 pm
• ACN/Water premixing - any benefits? July 21 - 01:56 pm
• Ghost peaks observed in Cation analysis July 1 - 05:34 pm
• I need a method/column for detergent analysis May 27 - 06:10 am
• Determination of Ascorbic acid-2-phosphate in tissue culture medium May 14 - 11:10 am
• Flow Injection Analysis July 8 - 10:13 am
• Shimadzu LC-10AT Pump July 7 - 12:55 pm
• Cation Exchange Column Needed July 1 - 05:44 pm
• Unidentified "Hump" present in ACN/Water gradient July 23 - 04:48 pm
• Sample Prep July 2 - 05:41 pm
• Need Alternative Column June 18 - 11:49 am
• Isomer separation July 24 - 08:45 pm
• C8 Columns-How reproducible are they within/between lots? July 1 - 06:20 am
• Bovine/human insulin July 5 - 09:22 am
• Degasification July 5 - 03:15 am
• Guard column July 7 - 04:36 pm
• Concentrations for Buffers and Ion-Pairing Reagents July 16 - 04:11 pm
• Pyruvate stability July 2 - 01:07 am
• Fluorescent Dye Carry-Over June 22 - 01:15 pm
• 0.4M Ammonium formate July 1 - 10:51 am
• Application July 19 - 11:22 am
• Organic Acid Analysis July 1 - 04:52 pm
• Ghost peaks appear with increased injection volume July 17 - 03:44 pm
• Vitamin E Acetate June 27 - 11:56 pm
• Loss of uv peaks when using gradient July 27 - 09:59 am
• Loss of Response of Hydrocodone bitartrate by HPLC December 4 - 02:57 pm
• What should I include in my Chemstation revalidation? July 6 - 02:46 am
• Evaporative Light Scattering Detectors July 28 - 04:01 pm
• GPC/SEC software July 5 - 02:51 pm
• Dyes June 26 - 08:49 am
• Amino acid analysis July 7 - 12:56 pm
• Microbial degradation of chloroaniline July 19 - 01:31 pm
• Air tight solvent bottles July 7 - 01:48 pm
• Where can I find syringe ion exchange filters / membranes? July 22 - 10:53 pm
• What is the purpose of TFA? July 15 - 09:26 am
• PAH's in sediment July 9 - 02:57 pm
• Chromatography of chlorophyll July 12 - 03:14 pm
• Column Flushing July 16 - 01:26 pm
• Bacterial growth in buffered mobile phase July 14 - 03:31 pm
• ESA coulometric detectors July 23 - 11:24 am
• Troubleshooting LC systems- advice on book purchase July 16 - 09:46 am
• Related compunds, maximum level. July 19 - 01:38 pm
• How wash the RP-Trimethy column July 20 - 08:04 am
• Lichrospher SC125 ? July 20 - 09:25 am
• Does anyone know the filling of the Ultrahydrogel 120 column July 20 - 05:17 pm
• Baseline problem at the tail of the peak. July 21 - 01:15 pm
• Just where is t0 measured ? July 28 - 12:10 pm
• Replace existing solvents completely in a HPLC pump April 11 - 01:15 pm
• New columns, Hybrid particle technology July 23 - 12:48 pm
• HP 1090 diaphram leaks oil July 29 - 06:23 am
• Proportioning Problems August 18 - 03:37 am
• Need retentive RP-HPLC column that is stable in 100% aqueous August 13 - 12:13 pm
• Internal standards and indirect detection in CZE July 15 - 01:38 am
• Shimadzu HPLC pump that eats seals and sapphire pistons August 17 - 11:39 am
• HPLC Detector calibration for range of use September 17 - 08:22 am
• Problematic gradients !! August 16 - 10:16 am
• Equipment cleaning validation August 4 - 08:28 am
• SFE-Supercritical Fluid Extraction August 3 - 09:33 am
• HBsAg Separation August 2 - 06:10 pm
• CN Columns using Ion-Pairing Reagents August 18 - 09:42 pm
• Last injection is larger or smaller than previous ones August 19 - 11:22 pm
• Fingerthight PEEK fittings to HPLC Column August 4 - 10:25 am
• Ideas for unusual separation August 13 - 09:19 pm
• How can I rid my sample solution of dissolved oxygen? August 6 - 02:03 pm
• Method Development for Extraction of Calcium,Magnesium and Potassium. September 27 - 07:47 am
• Wavering baseline in standard gradient run August 13 - 06:50 pm
• Interference Peaks August 9 - 02:19 pm
• Sonication only? August 23 - 02:39 pm
• Two-dimensional chromotography August 30 - 10:38 am
• Looking for a column August 9 - 01:53 pm
• What is the correct way to integrate closely eluting peaks? July 8 - 03:33 am
• RP Ion-Pairing Chromatographic Behavior August 7 - 07:50 am
• Increasing peak area August 12 - 07:02 am
• Extracolumn Effects August 13 - 10:34 pm
• Nomura Chemical Supplier/Equivalent August 14 - 01:38 pm
• Column Regeneration August 23 - 12:14 pm
• Any book of Chromatography Method Development available? Who Knows? August 13 - 08:40 pm
• I need a simple method for separating mono- disaccharides and amino acids from herbal extracts August 16 - 05:49 am
• Data System Sampling Rate August 17 - 12:00 pm
• Definition August 18 - 08:51 am
• Separation of kynurenine and kynurenic acid August 23 - 10:58 am
• Post-column fluorescence derivatization for anilines August 25 - 01:02 pm
• Ghost Peaks in Pure Methanol October 22 - 01:00 pm
• Column Volume August 20 - 01:10 pm
• Autozeroing the detector August 21 - 02:24 am
• Ghost Peak Originating from Buffer August 27 - 01:03 pm
• Making a 6N acid solution?? August 29 - 07:11 pm
• UPDATE on Abstract "humplike" Peak in blank methanol August 25 - 12:41 pm
• HPLC assay of Collagen August 26 - 05:00 am
• Hplc method for ambroxol hydrochloride November 10 - 11:28 am
• Separation of Furaltadone HCl and Amprolium HCl August 31 - 03:19 am
• Citrate analysis August 31 - 08:44 am
• Tocotrienol Std's - Merck Isomer Kit #8524 August 31 - 08:52 am
• Millenium Software/Peak Quantification October 5 - 04:46 pm
• Looking for HPLC method for crystal violet September 23 - 02:18 pm
• Baseline Sensitivity Problem due to Room Temperature October 9 - 04:28 am
• Chrom Merge October 14 - 12:22 pm
• Fraction Collector help September 30 - 02:37 pm
• McCalley papers on basic drug performance September 6 - 02:30 am
• Standard Solutions September 26 - 10:30 pm
• Remote starting Alliance September 2 - 10:34 am
• Problems with pumping pure ACN September 29 - 12:59 pm
• Hichrom column September 2 - 10:04 am
• Tests for columns September 7 - 01:04 pm
• What should be the limit of day to day variability of HPLC Assay September 20 - 06:19 am
• C8 degradation September 8 - 03:51 pm
• Curve Fit Type September 14 - 09:43 am
• How many analytes? September 13 - 09:17 pm
• Problems with KH2PO4 buffer September 14 - 06:57 am
• Methods for detecting caffiene. October 7 - 02:19 pm
• Determination of sorbitol in the presence of protein September 8 - 06:43 pm
• Changes in analytical method those require validation October 14 - 01:22 pm
• Cycling Baseline Noise during monitoring September 15 - 03:47 pm
• Detecting tertiary amines? November 14 - 01:50 pm
• Microbore chromatography: sensitivity/resolution September 12 - 11:02 pm
• Validation/Forced degradation analysis of formulations September 20 - 05:51 pm
• Determination of pK? June 19 - 09:26 pm
• Quantifying levels of ascorbate and dehydroascorbate in plant tissue September 22 - 04:55 am
• Waters Millenium Software (HPLC & GC) May 26 - 03:31 pm
• ISOCRATIC HPLC METHOD FOR PCR PRODUCTS September 23 - 10:39 am
• Negative deflections September 21 - 07:35 pm
• DIFFERENCE IN SENSITIVITY USING 2MM AND 3MM COLUMNS September 22 - 08:31 am
• SDS detection query September 23 - 06:47 am
• Need opinions on HPLC-ECD systems September 29 - 05:57 pm
• Method Development-How to know if a sample is ionized September 24 - 08:55 am
• Instrument calibration/validation parameters October 6 - 11:33 am
• Indirect conductivity detection September 23 - 02:34 pm
• Buffer September 29 - 12:43 pm
• CISAPRIDE September 30 - 03:50 am
• Germall 115 (Imidazolidinyl Urea) by HPLC January 29 - 04:05 am
• Problematic blanks !!!!!! October 15 - 05:23 am
• Papers on Radial Flow Chromatography September 1 - 05:51 pm
• Labpro Column Selectors with Millenium software October 31 - 01:26 pm
• Question on Linearity October 15 - 07:32 am
• Buffers and Salt Modifiers October 26 - 08:26 am
• Do we need a European Reference Column October 19 - 06:24 am
• Looking for a good HPLC troubleshooting book (or course) October 20 - 06:08 am
• How would you generate a free anime ? September 26 - 10:44 pm
• Quantitation using Evaporative Light-Scattering Detector? October 7 - 06:11 am
• Signal to Noise Ratio September 27 - 08:59 pm
• Washing columns with water October 6 - 06:45 pm
• Calibrating Instrument October 8 - 03:57 am
• LLOD/LLOQ September 29 - 06:44 pm
• Polymeric columns October 19 - 10:09 am
• Cationic Ion Pair Reagent July 25 - 02:52 pm
• Determination of MDA in biological samples April 23 - 04:03 pm
• Amino Acid Analysis Beckman 6300 October 18 - 08:25 am
• Column Choice October 20 - 03:21 am
• Sulfadiazine and Trimethoprim in feeds November 3 - 04:22 pm
• Needle Wash for Waters 2690 Alliance October 6 - 05:31 am
• TRANSITION METAL ANALYSIS USING CONDUCTIVITY DETECTOR October 7 - 01:22 pm
• Automation of SPE October 12 - 02:46 pm
• APPLICATIONS OF ION CHROMATOGRAPHY IN PHARMACEUTICAL INDUSTRIES October 15 - 07:20 pm
• Proanthocyanidins in Grape Seed Extract and Pine Bark August 13 - 04:36 am
• Reverse phase method for prednisone/prednisolone May 16 - 06:31 am
• Relative fluorescence quantum yield October 11 - 08:27 pm
• Baseline-problem with PDA October 24 - 04:05 am
• Why Use Chloride and Iodide Salts in TLC Mobile Phase? October 14 - 09:31 am
• Plasma sample preparation by protein precipitation October 19 - 11:01 pm
• Waters fraction collector problem October 15 - 02:14 pm
• Looking for an HPLC method for the assay of ethanol October 22 - 07:17 am
• CCC solvent pump October 29 - 02:39 am
• Phenylephrine HCl October 19 - 02:38 am
• Ammonium Acetate Buffer and Flourescence detection October 20 - 03:18 pm
• Vitamin D October 29 - 06:45 pm
• Isoeluotropic HPLC solvents October 29 - 12:36 pm
• Ion-pair reagent and strength of signal October 27 - 09:39 am
• Mm Equivalent for Ion Exchange (SCX) columns. October 21 - 07:23 pm
• Millenium 32 - Internal Standard Reporting October 21 - 04:12 am
• DIAMIR chromatography software query October 30 - 08:02 pm
• % Recovery? October 24 - 07:30 am
• Two peeks when changing the matris? October 28 - 12:46 am
• Tetrabutylammonium hydrogen sulfate October 26 - 07:30 pm
• Bufferchemicals October 27 - 10:07 am
• ELSD - Linearization of Signal September 25 - 11:59 pm
• Features of importance in Data aquisition software October 11 - 12:52 pm
• System Suitability November 15 - 12:21 pm
• Bufuralol intermediates September 15 - 03:36 am
• Photodiode array detector choice ? December 13 - 06:35 am
• HPLC analysis of silver sulphadiazine (SSD) September 17 - 08:10 am
• HPLC method for quantification of aminoguanidine in biological samples September 21 - 05:06 am
• Tocol Standard - Any suppliers ?? December 21 - 12:14 pm
• Formic acid Fouling September 24 - 10:13 am
• Benzethonium chloride assay November 18 - 02:55 pm
• In need of assistance for CE method for phosphate and glucorinic acid September 28 - 09:07 am
• How to reduce Chymotryptic like peptides in Map? September 29 - 12:02 pm
• Light Scattering (ELSD) Methanol/THF ghost peaks May 19 - 02:21 am
• HPLC data handling software December 22 - 09:04 am
• Separation or regeneration ?????? October 14 - 07:50 am
• Millenium 32 Default Wavelength November 5 - 11:00 am
• Estimating surface coverage December 19 - 07:13 pm
• Elution of various analgesics October 21 - 09:05 pm
• Analysis of a tertiary amine October 25 - 06:29 am
• Diffusion coefficient December 19 - 07:30 pm
• Analysis of asprin November 29 - 04:35 am
• Ion-Exchange chromatography November 1 - 06:22 pm
• Determination af sulphite by ionchromatography. October 29 - 05:03 am
• Analysis of pyredine derivat October 27 - 06:51 am
• HP chemstation printing problems December 1 - 05:43 am
• Urea Determination by HPLC October 28 - 11:15 am
• Biocad Sprint RPHPLC October 28 - 01:48 pm
• Phenyl-cyano column? May 30 - 11:11 am
• Waters 2690 versus HP 1100 December 6 - 02:27 pm
• I have a negative peak problem. December 7 - 07:09 am
• What is selective absorption? October 31 - 04:19 am
• HPLC Method for Green Tea to Determine Polyphenol Content. November 21 - 11:12 am
• PDA vs. ELSD - Quantitation issue November 2 - 09:20 am
• Looking for any HPLC assay info for Lutein or Zeaxanthin November 29 - 05:57 am
• Immobilized metal affinity chromatography December 3 - 01:15 pm
• Column Volume November 3 - 08:52 am
• ERGOSTEROL November 17 - 06:45 am
• TIAMULIN June 5 - 07:42 pm
• Phenylpropanolamin+brompheniramine+norpseudoephedrine November 9 - 09:38 am
• Question on Cleaning Validation November 17 - 05:04 pm
• Ion-pair determination of amino acids November 5 - 12:17 am
• Looking for a method for Glyphosate May 20 - 03:46 pm
• HPL analysis of oxalic acid and acetates November 17 - 04:05 am
• Column selection December 22 - 05:38 pm
• AZITHROMYCIN-ANALYSIS-PULSED ELECTROCEM-DETECTOR November 8 - 09:33 pm
• Calibration suitability November 10 - 01:11 pm
• Propylparaben November 12 - 12:26 am
• Best size silica/other product for Chromatography columns November 15 - 07:12 am
• PH adjusment of HPLC mobile phases December 22 - 02:22 pm
• TLC November 16 - 05:40 am
• Information on centrifugal partition chromatography November 16 - 05:28 am
• How to analyse sodium acetate using HPLC? November 30 - 12:20 pm
• Convert an SDS-PAGE method to CE method November 18 - 04:46 pm
• Looking for an HPLC method for the assay of ethanol July 14 - 12:42 am
• HPLC/GC problems with tertiary amines October 25 - 03:46 am
• Finding the concentration by the area under the graph November 22 - 09:29 am
• Priming injection : troubleshooting November 24 - 08:01 am
• SGE SS W5-C18 AR column December 8 - 02:22 am
• Bacterial growth inhibition by an organic modifier December 15 - 07:19 am
• Micromeritics 728 Autosampler Manual December 6 - 06:36 am
• Buffer crystalizing December 1 - 08:44 am
• Validation of Qualitative analytical methods December 2 - 06:34 pm
• HPLC method validation December 14 - 06:15 pm
• How to check the method capability? December 7 - 10:31 am
• Peaks eluting in void volume region. December 9 - 07:17 am
• Removing quaternary amines December 10 - 12:05 am
• Degassing mobile phase: He sparging vs. USP December 9 - 02:38 pm
• Detector saturation December 16 - 05:18 pm
• Paper Chromatography June 17 - 06:08 am
• Anyone using older (10+yrs) HPLC systems? March 2 - 02:24 am
• Decreasing response factors with increased concentration December 20 - 10:33 am
• Vitamin A Analysis. December 22 - 01:42 pm
• Transferability of gradient methods December 21 - 03:03 am
• Assay versus Method?!!! December 17 - 12:02 pm
• Urea Assay December 16 - 10:06 am
• Daily validation December 17 - 05:43 pm
• Cost-effective source of autosampler vials December 23 - 03:07 am
• Detectors December 14 - 04:02 pm
• TFA/ACN Degradation December 14 - 08:48 am
• Separation of fluoromethyl benzyl alcohol from cholromethyl benzyl alcohol December 14 - 12:50 pm
• Shodex kc-811 December 15 - 04:36 am
• Stress study for specificity validation May 25 - 10:41 pm
• How to Analyse active ingredient in blood/serum December 28 - 04:18 pm
• Determination of LOQ-LOD December 31 - 09:21 pm
• Detector problems for a beginner December 26 - 01:20 pm
• Looking for a column to seperate C , N and S December 3 - 09:26 am
• SPE method to quantitate hydrocodone and codeine by gcms December 6 - 06:20 pm
• Response factors problems with F-chemicals December 21 - 11:06 pm
• Method transfer protocol April 7 - 04:28 pm
• HPLC method for cetrimide October 22 - 05:14 am
• HELP/ADVICE needed -- Analysis software recommendations December 15 - 08:35 am
• Separation Problem: Need Some Suggestions November 27 - 09:00 am
• Limit of Detection/Quantitation December 30 - 10:20 pm
• Need help locating HPLC method for Cefuroxime Axetil July 1 - 03:03 am
• Chiral column January 10 - 08:52 am
• Purity factor and validation of impurity January 18 - 12:49 am
• Use of ECD in HPLC January 22 - 09:08 am
• Determine low levels of Teepol by HPLC June 8 - 04:34 am
• Retention time shift July 8 - 09:22 am
• Assay method-cetylpyridinium chloride? January 5 - 09:24 pm
• PAHs with HPLC May 11 - 01:45 am
• LOQ-LOD January 6 - 08:39 pm
• Related Substance Method Transfer January 27 - 07:31 am
• Calculation of related substances January 5 - 08:10 am
• HPLC assay of ketoconazole-help needed January 2 - 10:58 pm
• HPLC-column January 31 - 01:19 pm
• Integration of the peaks June 29 - 09:34 am
• Having big trouble with Vitamin K assay in the presence of Vitamin E May 9 - 02:31 am
• Selection of Buffers September 17 - 07:35 am
• 3rd (strong) mobile phase modifiers in RPLC February 23 - 12:55 am
• STOP PRESS STOP FLOW January 14 - 07:50 am
• %area vs %w/w January 17 - 12:44 pm
• Capturing 2D UV/Vis spectra from Chemstation files January 19 - 06:39 am
• HP 1090 Series II HPLC Troubleshooting January 21 - 08:07 am
• Symmetry Shield v Luna, HyPurity Elite etc January 21 - 08:14 am
• John Dolan's Book January 20 - 11:28 pm
• Peak tailing with basic compound April 16 - 02:25 pm
• Need info on LC Aspirin Assay in plasma June 30 - 11:48 pm
• Normalized Peak Area --??--Lioyd R. Snyder's HPLC Book January 27 - 06:00 am
• Mobile Phase Preparation January 27 - 11:15 am
• Ion exchange extraction of iodide February 17 - 02:58 pm
• Ion pair RP separation of phosphate, silicate, sulfate compounds February 15 - 05:37 pm
• How to export data HP-1100 ---> Excel spread sheet. March 6 - 05:46 pm
• Spectra Physics February 4 - 06:40 am
• LC method for Methylene Blue February 17 - 03:33 pm
• Quantitation problems for dextrose in presence of salts February 18 - 01:39 pm
• Systempeak March 15 - 05:29 am
• Ion Chromatography February 22 - 10:25 am
• HPLC conditions for a very polar guanidine derivative February 19 - 08:46 pm
• Peak Fronting May 23 - 04:22 pm
• A reverse phase column for Plasmid (5kb-9kb) characterization February 11 - 12:01 pm
• HP Diode Array Oddity March 7 - 11:16 am
• Sorbic acid and benzoic acid in cider February 17 - 07:21 am
• Buffer problems?? February 26 - 05:52 am
• HPLC UV Detecor vs UV photometer June 28 - 09:24 am
• Beer's Law March 11 - 08:09 am
• High Load, Low Load January 7 - 08:12 am
• Cocamidopropyl betaine February 21 - 07:58 am
• Chiral selectors in the RP mobile phases January 13 - 02:17 am
• Robustness in HPLC method validation April 13 - 01:01 am
• Gradient baseline March 7 - 11:34 am
• Ion pair chromatography. February 11 - 03:48 pm
• Degradation products for cough/cold formulations September 10 - 08:25 am
• ACCURACY OF THE METHOD - for related substances February 11 - 10:15 pm
• PEAK PURITY with WATERS PDA detector and Millenium 32 May 25 - 07:39 am
• XTERRA COLUMN-USP COLUMN January 25 - 02:10 am
• Help! Method info for calcium January 25 - 08:04 am
• LOD/LOQ form calibration curve March 17 - 01:27 pm
• Recovery problems in determination of riboflavin(vit b2)in dairy February 17 - 05:39 pm
• Should you Parafilm the top of your solvent bottle? February 2 - 03:46 pm
• Layman questions regarding Dionex, Waters, Agilent February 22 - 10:32 am
• Need opinions on ECD and Flourescence Detectors February 24 - 08:54 am
• Titanium by ion chromatography February 2 - 12:58 pm
• Decrease in HPLC flow yields large increase in peak areas February 1 - 10:35 am
• trace analysis of lineal alkyl benzene sulfonates by HPLC (e.g. TEEPOL) February 1 - 11:21 pm
• HPLC Colum Flushing February 5 - 01:52 am
• Shifting peak February 3 - 03:21 pm
• SPME HPLC February 21 - 06:58 am
• Dim but nice: Conversion of ppm to %(w/w) February 6 - 06:12 am
• Cleaning solvent filters February 11 - 11:05 am
• Waters 2700 Sample Manager question February 24 - 09:09 am
• Electrochemical detection(IC) for Glucosamine February 28 - 03:55 pm
• Surfactants mixtures March 22 - 11:18 am
• How to separate Aspartic acid and Ornithine February 17 - 04:59 pm
• Separation of lactose from maltose and sucrose February 11 - 10:07 am
• Chloraphyll A and Productivity February 11 - 05:17 pm
• Method Validation software March 6 - 05:59 pm
• HPLC Autosampler RSD problems February 16 - 07:56 pm
• Suggestions for manipulating Rt and Peak times February 15 - 07:25 pm
• Negative Peaks February 20 - 06:05 pm
• Back pressure too high February 16 - 10:53 am
• Linearity February 17 - 12:58 am
• Related substances May 26 - 04:38 am
• Doxycycline in porcine tissues February 18 - 07:24 pm
• Ion-pairing RP-HPLC for mutation analysis into DNA fragments May 27 - 08:45 am
• Preparative Chromatography February 18 - 12:39 pm
• Solvents for loading prep LC samples? March 3 - 03:32 pm
• Destroying the binding of drug to protein March 7 - 08:42 am
• Plant Chromatography Web Sites February 25 - 12:04 pm
• Finding a chromatography solvent February 24 - 05:42 am
• LC-Method for tetracyclines and/or penicillines March 4 - 12:57 pm
• Separation of Albuterol sulfate and phospholipids March 10 - 03:29 pm
• Priming injections February 25 - 12:40 am
• Methacrylic acid analysis June 1 - 03:46 am
• Mobile Phase Recycling March 10 - 02:27 pm
• LC-GC: The do's and do nots. March 8 - 01:50 pm
• How to compare various columns? March 12 - 09:54 pm
• Do we have to perform additional validation for an equivalent column March 8 - 08:40 am
• Clean-Up Method for HPLC ? Need Advice Fast!!!! March 6 - 01:02 am
• Column dead time HELP! February 29 - 07:19 pm
• HPLC methods for Opiates March 2 - 03:18 am
• Column equyilibration with THF March 20 - 08:28 am
• Polar compounds March 6 - 01:45 pm
• Ionstrenght: Samples and mobilephase March 5 - 09:46 pm
• Retention time shift March 14 - 07:26 am
• Peak Purity on HP1100 DAD March 8 - 11:17 am
• Nitric acid for HPLC cleaning May 18 - 04:27 pm
• Retension time getting shorter March 10 - 02:18 pm
• Postcolumn conditions to make UV or fluorescent derivatives April 5 - 03:50 pm
• HPLC Method for Comparing Instruments March 13 - 07:32 pm
• HPLC columns for peptide mapping March 9 - 03:27 pm
• Analysis for basic polar analyte in 100% methanol? March 10 - 03:47 pm
• Vanillylmandelic Acid (VMA) March 10 - 03:54 pm
• Wavy baseline (uv detector) March 16 - 10:07 am
• Preventative maintenance/re-calibration of HPLCs. What and how often is needed? March 16 - 09:07 am
• HPLC analysis of Deoxyalliin May 10 - 11:40 am
• To all method validation experts out there.... May 11 - 06:13 am
• Column washing March 20 - 08:31 am
• Column Washing with TFA March 24 - 08:02 am
• Practical info for validating HPLC method March 23 - 04:02 pm
• Method to analyze by HPLC non-chromaphore bicyclic compound March 27 - 04:04 pm
• Waters Millenium Software & HP5890 II March 6 - 11:26 am
• Dibuthyl phthalate February 16 - 02:45 pm
• Cocamidopropyl betaine January 12 - 04:36 pm
• Testing of amino acids November 19 - 06:46 am
• Phenyl-Hexyl Phase for HPLC May 16 - 02:32 pm
• TFA turns yellow? April 17 - 11:02 am
• Separation of plasmid DNA on HPLC May 24 - 08:51 am
• A reverse phase column for Plasmid (5kb-9kb) characterization April 17 - 01:55 pm
• Gradient baseline irregularity April 14 - 09:54 am
• LC-GC combo applications March 8 - 02:45 pm
• Igor macros for Chromatograms? March 9 - 05:18 pm
• SEC/HPLC of low molecular weight (5-7K) Heparin May 10 - 09:13 am
• CIP-buffer with sarcosyl for cleaning streamlinecolumn (Amersham Pharmacia) March 12 - 04:37 am
• Experience with Nitrogen specific Detector in HPLC March 14 - 01:56 am
• GLP question regarding cutting of the weight April 18 - 05:08 am
• Bizarre LC problem, comments (very!) welcome April 28 - 08:40 pm
• Three peaks and three different %RSDs March 23 - 04:26 pm
• Analyse protected pyranose March 27 - 11:40 pm
• LC plasma carnitine method March 24 - 08:23 am
• Normalized Peak Area March 24 - 09:09 am
• Possible matrix effect, but only on a certain LC! HELP! March 29 - 05:17 am
• Heme-protein isolation. March 28 - 05:32 am
• Lipid hydroperoxide purification March 28 - 05:37 am
• HPLC pepsin digest resolution of haem-peptide fragment April 4 - 11:36 am
• Problem with EC detection March 31 - 02:44 pm
• Methods for Germall (imidazolidinyl urea, or imidurea) March 29 - 07:32 am
• Matrix effect?!!!!! Please Clarify March 30 - 07:57 am
• Chiral d/l amphetamine separations: LC or GC best?? May 31 - 05:32 pm
• Peak Area Variations April 3 - 01:45 pm
• Hydrofobic charge induction chromatography April 4 - 06:36 am
• Cosmetic Preservatives April 13 - 08:09 am
• Ammonium Acetate buffer July 1 - 02:22 am
• Quantitative detection of Sodium salt April 6 - 09:32 pm
• 3 micron columns May 4 - 05:58 am
• Frontal Analysis April 17 - 12:11 pm
• Van Deemter: H/dp < 2? May 26 - 08:15 am
• HPLC analysis for Neomycin, Bacitracin and Polymyxin May 17 - 01:45 pm
• S/N problem April 17 - 12:29 pm
• BUFFER USED IN LC April 17 - 09:01 pm
• Chromatography data systems May 2 - 08:37 am
• In-line pH measurement for HPLC applications April 18 - 12:30 pm
• Microboard Column April 19 - 03:51 pm
• Method validation May 22 - 02:40 pm
• UV Detection April 21 - 04:48 pm
• Selection of column based on chemistry April 24 - 02:13 pm
• Assay versus content uniformity April 24 - 10:03 am
• HPLC of sugars (maltose). April 11 - 11:50 am
• Quantitative HPLC analysis of impurities May 23 - 07:24 pm
• Method for sodium acetate in presence of sorbitol and protein May 25 - 11:52 pm
• Pre-run system suitability question April 28 - 04:29 pm
• Alternative HPLC method for human insulin? May 19 - 10:20 am
• Qualification for a refractive index detector April 28 - 09:03 pm
• Linear alkylbenzensulfonat and HPLC May 25 - 11:01 pm
• High Backpressure with THF. April 28 - 12:19 pm
• LC Separation of very closely related peptides May 10 - 12:14 pm
• Priming injections May 1 - 11:54 pm
• Ion pair separation of LAS on C18 May 25 - 11:09 pm
• Columns for basic compounds May 11 - 05:26 am
• HPLC "Calibration" for cGMP May 10 - 08:00 am
• Compound for Detector wavelength verification May 23 - 07:00 pm
• Check standards May 13 - 12:10 am
• Cholesterol HPLC April 12 - 08:59 am
• Analysis of Formulation Containing Miglyol 840 May 16 - 12:28 pm
• PREDNISONE & PREDNISOLONE DETECTION BY HPLC-UV May 19 - 03:42 pm
• Test Mix Reference May 25 - 08:36 am
• Coelution of Impurities May 29 - 01:02 pm
• Troubleshooting - injection error May 22 - 01:20 am
• Unexplained peak using cation-exchange method May 25 - 02:11 pm
• Post column derivatisation vs precolumn derivatisation May 26 - 02:36 pm
• Resolution Problem May 2 - 11:09 am
• Separating large biomolecules (200kDa) May 31 - 02:04 pm
• RI Detector injection peak April 28 - 10:35 am
• Pigment problems March 1 - 04:37 pm
• Ion chromatograaphy March 3 - 07:57 am
• Glycerin detection March 13 - 04:23 am
• Igor macros for Chromatograms? March 9 - 11:59 pm
• Internal Standard May 15 - 08:06 am
• Method to quanitate mineral oil May 25 - 05:12 am
• Shelf life of THF February 23 - 06:35 am
• HP1100 problem March 8 - 11:27 am
• The use of IAM chromatography January 9 - 12:55 pm
• Export data from PC1000. January 21 - 12:00 am
• Allantoin Method!! April 12 - 05:11 am
• Benzoic acid October 16 - 05:52 pm
• Mistery peak on gradient December 3 - 02:59 pm
• Using an Unretained Compound for Determining Column Void Volume November 17 - 04:43 pm
• FMOC derivatization problem November 27 - 12:52 am
• High HPLC column pressure June 2 - 12:03 am
• METHOD VALIDATION December 5 - 10:02 am
• Determination of Benzalkonium Chloride in Lecithin Matrix - Please Help! November 1 - 02:27 pm
• Transfer of Analytical HPLC Methods December 7 - 06:49 pm
• Degassing the mobile phase December 7 - 12:34 pm
• RP separation of alkylsulfonates : mechanism ? November 1 - 07:04 am
• Sample carryover from sample loop? November 29 - 06:26 am
• Turbo HPLC October 4 - 01:27 pm
• Waters 2690 and Gradients October 2 - 12:29 pm
• Ion Exclusion question March 30 - 10:03 am
• HPLC gradient/isocratic run October 3 - 06:33 am
• Out-of-specification results / integration parameters October 7 - 03:56 pm
• External vs. internal standard calibration October 27 - 08:27 pm
• Gradient dwell time June 27 - 09:47 pm
• RP-C30 October 25 - 06:35 pm
• Sucrose removal October 4 - 05:19 am
• Neomycin sulphate December 8 - 12:48 pm
• UV and Fluorescence Detector Linearity Range October 9 - 10:27 pm
• Pair ion HPLC of nicotine June 29 - 09:05 am
• Problems with chromatography October 10 - 07:27 am
• LC for methyl salicylate October 10 - 01:10 pm
• Cis-trans isomerism by UV detection October 12 - 04:26 am
• Low-MW Dextran SEC/GPC October 10 - 05:45 pm
• Artifact peaks from 25mM KH2PO4 , pH 3.0 October 10 - 05:49 pm
• Peak Tailing October 16 - 10:05 pm
• What kinds of molecules have fluorescence? Any theory? October 16 - 02:17 am
• Gel filtration October 16 - 01:05 am
• Methods of separating water soluble vitamins by HPLC December 7 - 06:31 am
• Determination of chloride salt in pharmaceutical substance October 18 - 10:03 pm
• Is Preparative HPLC purification of stearyl amine possible ? October 18 - 03:05 pm
• Linearity Solution Composition November 8 - 12:45 pm
• Preparation of protein samples for amino acid analysis?? November 7 - 10:00 am
• HELP ! Bubbles forming during acetonitrile gradient. October 28 - 07:07 pm
• Toluene in Non-polar matrix October 24 - 02:37 pm
• HPLC Optimization Softwares October 28 - 07:13 pm
• Amino Acid Assay After Derivatization April 15 - 07:30 am
• Using Perchloric Acid in mobile phase October 27 - 08:02 am
• Solutions in the lab October 24 - 12:41 pm
• Ion-exchange columns SCX October 25 - 06:39 pm
• Pectin citrus October 27 - 08:13 am
• Pressure fluctuations October 28 - 06:34 pm
• HP 1100 noisy baseline November 16 - 02:28 pm
• Large baselinedrift with refractive index detector November 2 - 05:13 am
• How to Find an FDA Document October 26 - 05:54 pm
• Sample Prep-Centrifuge Speed October 30 - 04:46 pm
• Is rpm still a valid unit for centrifugation speed? November 17 - 01:17 am
• Trace Analysis of Benzotriazole May 29 - 10:35 am
• 4 minutes broad peaks November 8 - 05:25 pm
• Shareware or Freeware Integration Software November 2 - 03:00 am
• HPLC problems June 2 - 12:08 am
• How much MeCN is safe on a Shodex KC-811? July 22 - 06:29 am
• Glycosphingolipids? December 18 - 11:57 am
• HPLC Fittings November 3 - 06:37 am
• Methyl methacrylate analysis???? November 7 - 09:57 am
• Ipratropium bromide November 17 - 12:52 pm
• Statistical tools used for intermediate precison November 9 - 05:04 am
• Anomalous (?) Gel Filtration November 24 - 07:48 am
• Measurement of piperazine in air November 10 - 03:04 pm
• Volatile buffer for acid antibiotic November 16 - 08:39 am
• HCl neutralization November 15 - 07:25 pm
• K-bar instead of K-prime November 16 - 12:17 am
• Heated Transfer Lines for SEC-TREF November 16 - 09:09 am
• Increasing HPLC throughput November 20 - 06:53 am
• Chloride analysis by ion chromatography June 17 - 05:58 am
• Ambient November 23 - 01:44 am
• Interfering peak in blank gradient December 3 - 03:24 pm
• Primary standard purity calculation November 26 - 03:53 am
• Buffers at 3.7 with low Abs. at 240 nm December 6 - 04:19 pm
• Separation of macrolides November 27 - 10:03 am
• Hexane as ion pairing November 29 - 07:11 pm
• Ammelide by HPLC anyone ??????? September 9 - 02:46 pm
• Drugs of Abuse December 6 - 10:06 am
• Malondialdehyd with hplc December 19 - 12:33 pm
• Chromatography Data Systems December 16 - 09:48 am
• Suggest me an autosampler December 12 - 02:32 am
• HPLC of RESIDRONATE December 8 - 06:10 am
• Quantitation of Peaks in the 0.1 - 1% Range December 26 - 07:39 pm
• Plant pigments and chromatography December 11 - 08:22 pm
• Ergosterol analysis by HPLC December 18 - 08:09 am
• Re-equilibrating gradients December 12 - 09:50 am
• Re: Huge and sudden drop in fluorescence during gradient HPLC March 10 - 07:26 am
• Experation date for solutions December 14 - 01:48 pm
• THF in mobile phase and Baseline drift, please help!! December 20 - 07:34 am
• Scale-up issues December 18 - 10:45 am
• RRTs shifting in gradiet method December 21 - 03:49 pm
• Negative peak in isocratic method July 1 - 01:49 am
• LC column info December 21 - 11:28 pm
• Retention Time Shift December 22 - 01:15 pm
• Analysis of samples in n-Octanol by HPLC December 27 - 07:18 am
• How to calculate g-1 from rpm October 2 - 04:48 pm
• Horwitz formula October 19 - 11:06 pm
• DGGE with HPLC? December 4 - 07:18 am
• Ghost peak issue January 20 - 09:03 am
• Differences between amperometric and coulometric ECD January 19 - 02:01 am
• Method Verification Problem January 19 - 02:05 am
• Algae formation in water mobile phase/pH electrodes January 4 - 11:59 am
• Biotek-Kontron or Kontron HPLC equipment January 19 - 02:10 am
• Retention time continuously decreased June 8 - 12:46 am
• Determination of glyphosate in water January 12 - 02:42 am
• Torsemide, Furosemide, any fluorescence? January 6 - 08:20 pm
• GPC in Acetone January 29 - 08:19 am
• GFC column calibration January 5 - 04:38 pm
• ELSD info? June 2 - 07:28 am
• Separation of secondary and tertianary hydrophobic amines February 28 - 12:54 pm
• Vancomycine - HPLC January 17 - 01:25 am
• End capped columns February 6 - 01:01 pm
• Why does flow rate affect peak area? January 5 - 03:49 am
• Which HPLC system is best for protein analysis? May 23 - 09:06 pm
• Retention time variation January 2 - 08:37 am
• Polymeric content January 5 - 03:04 pm
• Problems with analyte carry-over January 10 - 05:05 am
• Analysis of basic, hydrophobic peptides January 30 - 05:35 am
• LOQ minimum concentration January 9 - 11:37 am
• Blocked frit February 22 - 08:55 am
• Compacted gel January 10 - 12:10 pm
• Response Factor February 6 - 04:30 am
• Phase collapse February 19 - 05:21 pm
• Fronting peak and poor recovery at low concentrations January 25 - 11:47 pm
• Zero Intercept for Cal Plots January 18 - 12:33 pm
• RP-HPLC Column Equilibration June 5 - 09:50 am
• Lofepramine from serum July 18 - 07:33 am
• Fluorouracil and Buffer vs. Water for Mobile Phase January 24 - 12:21 am
• Alpha Lipoic acid and Pregnenolone January 23 - 08:03 am
• Area Under Curve Units January 24 - 06:32 pm
• Old double destilation with MnO4K, could anybodie explain that method? January 24 - 01:33 pm
• Retention time shift January 25 - 03:56 pm
• Alcohols and pH August 2 - 06:48 pm
• SymmetryShield for sulfa drugs January 31 - 06:00 am
• Narrow-bore HPLC columns - resolution January 29 - 02:48 pm
• Narrow-bore HPLC columns - resolution February 28 - 05:46 pm
• Normal phase chromatography June 4 - 02:41 pm
• Negative peaks January 25 - 11:35 pm
• Porphyrin Analysis February 27 - 01:53 am
• Wavelenght justifiactions February 1 - 11:07 am
• Refractive index and electric current January 29 - 10:00 am
• Precolumn labelling January 30 - 08:33 am
• Chromatography software February 5 - 06:13 pm
• Chromatographie of organic pigments and dyes June 17 - 05:34 am
• HPTLC February 16 - 02:24 am
• Any HPLC methods for separation and identification of Maillard reaction compounds ? March 21 - 09:59 am
• Validation / Accuracy question March 16 - 01:37 pm
• TLC SPRAY REAGENT March 30 - 11:41 am
• Clinical applications of HPLC July 18 - 08:11 am
• GPC-Molecular Weight Distribuiton Plot February 1 - 11:18 am
• HPLC method development software February 6 - 11:15 am
• Tailing peak in ion pair RP chromatography February 6 - 01:18 am
• TLC Spray Reagent January 25 - 08:46 am
• GPC-Molecular Weight Distribuiton Plot February 6 - 07:04 pm
• Problems with test for related substances of Apramycin February 5 - 01:01 am
• Doxycycline standars February 8 - 07:58 am
• Trimethoprim by HPLC February 18 - 09:56 am
• Agilent/HP1050 LC series service and repair February 13 - 08:49 am
• Impurities in methanol February 7 - 10:56 am
• Method for Sodium Acetate February 12 - 03:35 pm
• Sulfinic and sulfonic acids separation May 28 - 10:54 am
• Ghost peak source? February 8 - 03:15 am
• Acetic acid, trifluoro- vs trichloro- February 9 - 07:38 am
• Sample preparation for hplc February 9 - 11:09 am
• HPLC method for a compound with pKa 2.3 and 9 February 15 - 09:53 am
• Determination of sulfonamides in meat February 8 - 05:19 pm
• Maleic acid/ Fumaric acid February 12 - 09:52 am
• Hydrophilic Interaction Chromatography February 15 - 02:03 pm
• Linear regression vs response factor May 15 - 08:12 am
• Acidic and base separation August 8 - 03:34 am
• Angelica lactone October 11 - 08:13 am
• Chromatography about the lipids October 4 - 07:51 am
• Determining the component percentages of solvent blends. October 11 - 08:11 am
• Peak tailing October 16 - 09:40 am
• Software that reads ASCII data from a serial port - of use to you? October 19 - 07:37 am
• Phospholipid analysis? April 13 - 08:06 am
• Removal of Ion Pairing Agent from Collected Fractions. April 18 - 10:02 am
• Determination of Matrine(Alkaloid) by HPLC June 8 - 06:05 pm
• Pancuronium Bromide Peak Shape March 2 - 09:36 am
• Identification by HPLC Retention Time March 30 - 09:33 pm
• Peak purity interpretation using PDA May 4 - 10:03 am
• ELSD info? March 13 - 02:07 pm
• Hydrophobic collapse April 17 - 10:44 pm
• Variable volume injection for quantitative analysis May 25 - 10:04 am
• Column May 8 - 08:56 am
• Erythromycin HPLC Assay June 22 - 05:32 am
• Affect of pH? April 16 - 12:56 pm
• Flash Chromatography January 21 - 12:56 am
• Solvent elution strength May 3 - 01:26 pm
• METHOD VALIDATION April 27 - 02:45 am
• HPLC method for L-carnitine March 12 - 04:08 pm
• Is RI with a Reverse Phase gradient possible? March 12 - 04:26 pm
• Problems Getting Reproducible Areas in Samples April 6 - 07:21 am
• Ion Chromatography August 20 - 07:53 am
• Determination of protein in Polysaccharide (Alginate) May 14 - 12:14 am
• Ion-pairing column clean-up April 3 - 10:33 am
• HPLC of Bromo Compounds February 10 - 03:26 am
• Agilent/HP 1100 and 21CFR11 April 25 - 12:16 pm
• Hydrophobic Interaction Chromatography (HIC) February 28 - 05:24 pm
• LC Method for detecting MTBE in tap water. February 13 - 08:36 am
• Anion Exchange Column February 20 - 04:53 pm
• Tailing Aldehyde Peak February 14 - 03:10 pm
• Comparison of two sets of data February 15 - 10:02 am
• Assay Method for Nuosept C (a preservative) February 14 - 07:08 am
• Guard column stability February 17 - 04:11 am
• 6-beta-OH-cortisole February 16 - 06:23 am
• Fluconazole, any fluorescence? February 22 - 11:16 am
• UV-Vis absorbance detector vs. photodiode array detector February 20 - 04:50 am
• Which chiral columns to choose ? March 19 - 06:06 pm
• PL Gel or Zorbaz for GPC? March 3 - 12:58 pm
• Agilent vs MacMod Zorbax Columns March 7 - 02:36 pm
• Ion Pairing: Retention Continuously Increased? February 27 - 06:52 am
• HPLC in plants studies; increasing column life February 28 - 04:30 pm
• Baseline Correction in Gradient HPLC March 7 - 04:58 am
• Gradient baseline/ghost peaks/strongly retained impurites March 3 - 10:06 am
• LC of gluconic acid April 10 - 03:45 pm
• Ion Pairing- Reference Article/Text Recommendation? July 18 - 01:13 pm
• Negative deflections and electrochemical detection June 29 - 04:52 pm
• Clean up methods for small volumes March 2 - 03:32 pm
• Masking agents in RP gradient HPLC April 4 - 06:08 pm
• Retention time variability March 12 - 10:55 am
• Drug stability and sample solution stability March 8 - 10:12 am
• LC column acidity March 7 - 04:27 pm
• Periodically occuring peaks when monitoring baseline March 21 - 06:04 am
• Flumazenil HPLC Assay March 11 - 12:47 pm
• Accuracy at LOQ for impurity methods including clenaing methods March 12 - 08:39 am
• How to choose ISTDs for bioanalytical HPLC methods March 17 - 06:02 pm
• Should Flow Rate Effect Detector Response Like This? April 4 - 06:25 pm
• How does beffer concentration affect resolution? March 14 - 04:17 pm
• Separation of folding intermediates March 14 - 04:56 pm
• Buffer Preparation March 14 - 08:59 pm
• How do co-eluting compounds affect UV signal? March 17 - 06:15 pm
• Negative peaks with Diode array March 21 - 07:34 am
• Selenonurea by HPLC ? March 15 - 08:25 am
• CLEANIN VALIDATION FORMULAS ?????? April 22 - 09:30 am
• Paper chromatography March 17 - 03:35 pm
• HPLC pump validation tests May 15 - 04:51 pm
• Digital Liquid flow meter for HPLC March 21 - 08:49 am
• HPLC for DEOXYPYRIDINOLINE March 22 - 06:28 am
• Ruggedness March 27 - 07:18 pm
• Gradual flow increase March 29 - 03:24 pm
• Turbochrome Navigator software problem March 31 - 09:43 pm
• System Suitablity for Protein QC Methods March 23 - 09:31 am
• Buying a new HPLC system; what should I do? March 31 - 09:32 pm
• Pressure Change During Isocratic Run March 28 - 05:40 am
• PH of sample--split peaks April 12 - 05:24 am
• Asimmetry factor March 29 - 10:38 pm
• UV Cutoff Below 190? March 29 - 08:59 pm
• Dimethyloctylamine pH range? April 11 - 05:31 pm
• LOD for TLC Dragendorff's Reagent March 15 - 06:01 am
• Ion pairing and optimum pH April 9 - 05:06 pm
• Solvent recycling........ April 3 - 04:02 pm
• Modified starch analysis by GPC!!! May 1 - 12:02 pm
• Mobile phase April 11 - 12:34 pm
• Mass balance calculations April 3 - 12:54 am
• Turbulent flow chromatography. June 21 - 04:01 pm
• Method Transfer (HPLC/GC) April 14 - 05:17 pm
• Baseline drift April 6 - 12:42 am
• HPLC OQ April 5 - 03:31 pm
• Assay of silicone dioxide April 13 - 11:59 am
• Iso-nicotinic acid hydrazide April 8 - 09:42 am
• Intermittent Instrument Failure April 5 - 03:14 pm
• The LiPF6 and solvent (like ethylene carbonate) analysis by HPLC!!!!! April 5 - 05:08 am
• Comparisons of Drylab software and MODDE, what are the differences?? February 11 - 03:10 am
• Chiral analysis April 5 - 04:04 pm
• chiral hplc analysis April 6 - 01:46 pm
• Polar embedded group April 6 - 10:15 am
• Is hplc of acarbose antidiabets by uv detection is possible and how July 7 - 09:30 am
• How to measure resolution? April 6 - 09:35 am
• Cyanocobalamin (B12) May 24 - 08:40 am
• Carry over in protein HPLC analysis April 9 - 06:55 am
• System Backpressure April 9 - 10:27 am
• Extracolumn effects April 11 - 07:12 pm
• Calbration Curve April 8 - 07:41 am
• HPLC Detectors May 24 - 09:31 pm
• Setting up a stability protocal August 11 - 10:57 am
• Increasing retention times April 11 - 01:04 pm
• Analysis of Phosphocholines April 11 - 08:50 am
• Ion chromatography April 10 - 08:21 pm
• Ion pair chromatography April 11 - 03:16 pm
• Seeking advice on data acquisition systems - ChemStation or TurboChrom? April 25 - 08:03 am
• IS IT POSSIBLE TO DO ANALYSIS WITH A NEGATIVE April 14 - 05:32 pm
• Broadened peak/poor resolution April 12 - 10:38 am
• Decreasing retention times April 18 - 07:59 am
• 21 CFR Part 11 April 12 - 01:31 pm
• Fluconazole HPLC Methods April 13 - 05:08 am
• Salmeterol April 13 - 09:49 am
• Cyclodextrines - univeral method April 16 - 10:25 am
• Where to find pHa April 19 - 03:33 pm
• HPLC of organic acids, aldehydes and ketones April 18 - 10:06 am
• Bulk capture purification May 3 - 01:38 pm
• Impurity detection and s/n ratio. April 25 - 02:01 pm
• Base line derive May 2 - 02:54 pm
• Lithocholsäure April 22 - 10:32 am
• Decreasing standard peak's area count? April 20 - 10:53 am
• Positive baseline drift April 20 - 07:56 am
• Hey I.D. you forgot to write what is Se under "CLEANING VALIDATION FORMULAS" DATED March 26 May 11 - 06:47 pm
• Peak before dead time... April 25 - 07:54 pm
• Peak Purity April 23 - 10:45 am
• Post Column Reactors April 30 - 02:57 pm
• Post Column Reactors April 24 - 06:27 am
• Signal to noise ratio May 24 - 08:10 am
• Wavelength accuracy test on fluorescence detectors April 24 - 06:54 pm
• Preparative Chiral Separations. May 1 - 08:37 am
• Call for molecules knowledge February 29 - 02:38 am
• 21 CFR part 11 -- need to save interim integration results? May 12 - 07:34 pm
• Pka value May 3 - 06:59 am
• Noise level April 26 - 07:46 am
• Max. Column Size for Normal HPLC System April 27 - 11:02 am
• Measurement of individual peak areas of overlapped peaks April 27 - 12:17 am
• Calculate %RSD in Excel May 9 - 09:29 am
• Comparison of hydrophobicity of c8, c18 and PS-DVS May 10 - 01:10 am
• Beta-lactam standard May 7 - 07:54 am
• Tropin Alcaloids May 7 - 01:32 pm
• LC surfactant polymer. Please HEALP!! May 10 - 11:04 am
• Sennosides standards (C-G) May 18 - 08:34 am
• Separation of GST-fusion protein from its partial degraded product May 11 - 12:03 am
• Mobile phase recipe May 10 - 08:11 am
• Method Development Questions (mobile phase/sample prep) April 21 - 03:52 am
• Baseline drop in ACN:H2O:TFA system May 18 - 07:25 am
• Identification of degradation products for IND and NDA? May 16 - 06:17 am
• Sample Injection volume? May 11 - 12:47 pm
• Internal Standard for PAH analysis May 21 - 10:39 pm
• Need name/supplier for HPLC accessory please! May 14 - 11:39 pm
• IQ, OQ, PQ and Calibration May 11 - 07:41 pm
• Gilson 321/322 HPLC pumps May 31 - 05:47 am
• Buffer for size exclusion May 15 - 03:31 pm
• Mystery Peak June 7 - 01:59 pm
• Taurine Assay May 16 - 05:58 am
• Separation of peptides from simple sugars and organic acids May 17 - 03:16 pm
• HPLC H2O May 22 - 01:41 pm
• Hitachi HPLC Series 7000 Purchase? Good or Bad Idea? May 11 - 01:36 pm
• "aqua columns" May 25 - 06:54 pm
• 2-naphthylamine analysis May 18 - 09:00 am
• Should I buy an Agilent 1100? May 31 - 02:41 am
• COLUMN USED IN REVERSE??? May 22 - 03:38 pm
• Avoparcin by HPLC May 23 - 05:47 am
• Acetonitrile HPLC waste May 10 - 11:30 am
• Limit of detection and quantification June 17 - 07:08 pm
• HPLC Aftermarket Mixer May 22 - 11:11 am
• Between Waters and Varian May 29 - 08:42 am
• Avoparcin by ion-pair chromatography May 23 - 02:41 pm
• Analysis of Cefdinir for related substances in the bulk drug. May 23 - 05:55 am
• Validation of dissolution method May 31 - 01:40 am
• System Suitability May 13 - 11:16 pm
• Amoxicillin in feedstuffs May 24 - 12:59 pm
• Methodology questions June 1 - 10:50 am
• Replicate analysis and method validations May 25 - 03:45 am
• HPLC May 29 - 05:37 am
• Laboratory Power Protection for Analytical Instrumentation May 29 - 11:56 pm
• SP8780 AutoSampler June 21 - 08:34 pm
• Single RP HPLC method to quantify metformin and glyburide in pharmaceutical preparations June 22 - 05:42 am
• How to mesure Vd in LC? June 8 - 05:09 am
• Q: Nova-Pak C18 column. June 4 - 03:04 pm
• Column Wash! When can it "kill" a column? June 1 - 08:29 am
• Acetonitrile polymerization on HPLC system? June 19 - 11:43 pm
• Anormal high value for only one component out of three during method transfert May 31 - 08:09 am
• Can I improve bipyridine peak shape? May 31 - 02:56 pm
• Q: HPLC and LABNET June 5 - 07:29 pm
• WHAT IS THE REASON BEHIND THE PEAK FORNTING AND PEAK TAILING AND HOW IT CAN BE SOLVED June 1 - 12:31 pm
• Unwanted protein co-purification by affinity chromatography June 1 - 02:38 pm
• N-nitrosodiethanolamine June 1 - 12:46 pm
• Tannins of Terminalia chebula June 4 - 03:58 pm
• Qualification June 6 - 02:24 am
• PKa June 5 - 02:16 pm
• Decreasing Retention Time, Increasing Standard Peak Areas June 8 - 07:44 am
• Problems with Sodium Sulfadimethoxine New HPLC Method in USP June 6 - 07:33 am
• Cyanogenic Glycoside Purification June 6 - 09:37 pm
• HPLC relationship between flow and retentiontime June 7 - 11:38 am
• Gradient elution June 13 - 08:37 pm
• Baseline positive drifting of ELSD without column June 14 - 12:06 am
• How to seperate methacrylicacid fom acrylacid with hplc June 14 - 04:54 am
• HFE-300 or Ethoxynonafluorobutan June 8 - 05:45 am
• Fast Method Development June 11 - 12:04 pm
• Resolution between two closely absorbing species June 18 - 06:20 pm
• SPE cartriges for desalting peptides June 18 - 11:34 am
• Ion pair chromatography June 11 - 03:28 pm
• Peak shape problem because of contaminations ? June 14 - 01:00 am
• Monolithic Column June 19 - 05:55 am
• Separation of Potasium Sorbate and Sodium Benzoate in a mixture June 15 - 06:43 pm
• Agilent 1100 Problems June 20 - 07:11 am
• Sibutramine analysis by hplc June 22 - 05:28 am
• Variable Rfs for phospholipids in TLC June 19 - 11:56 pm
• Looking for Nomura Chemical Co. June 18 - 05:15 pm
• Method Transfer Problem June 21 - 02:24 pm
• Millennium and LIMS interface? June 19 - 10:54 am
• Q: Pressure change during column cleaning. June 24 - 07:38 am
• Column plugged with salts? June 20 - 12:18 am
• HP1100 frequent frit change June 22 - 08:35 am
• HPLC of small keto acids June 27 - 07:40 pm
• HPLC VS HPTLC June 21 - 04:43 am
• Problem with DAD detector June 24 - 11:43 pm
• Agilent 1100 software question, 3D FL scans June 28 - 05:39 am
• Protein binding in biological samples June 26 - 12:16 am
• Hydrophobic interaction June 28 - 05:58 am
• Decreasing retention times June 28 - 09:04 am
• 21 CFR Part 11 June 28 - 02:00 pm
• Peak purity with HP1100 DAD June 29 - 04:57 pm
• Chromatography Software July 26 - 12:15 am
• Help/Information on GC May 26 - 05:13 pm
• Supplier for deuterated Hexanal and/or deuterated standards in general ?? June 14 - 08:37 pm
• Packed Columns and the USP June 13 - 12:45 pm
• Estrogen analysis June 18 - 12:01 pm
• Alkyl phosphate metabolites June 19 - 07:45 am
• Acid oleic June 25 - 03:27 am
• Is it possible to analyis thioctic acid and l-ornithine l-aspertate by using single chromatographic system June 11 - 05:53 am
• Glycolic acid July 16 - 09:07 am
• Cleaning ESA guardcell 5020 June 21 - 10:54 am
• Mehod of analyis for thioctic acid and l-ornithine l-aspertate(lola)in combination June 22 - 05:38 am
• Dimethylglioxime Analysis June 27 - 03:54 am
• GPC analysis using Millennium 32 June 29 - 09:25 pm
• HPLC of Cinitapride February 20 - 09:06 am
• How to evaporate highly aqueous eluate? March 11 - 03:42 pm
• Ion Exchange Chromatography April 7 - 12:06 am
• Liquid Chromatography April 2 - 10:06 pm
• Single broad "peaks" during ECD of catechol amines April 10 - 04:17 pm
• Is it possible to analyse chondroitin sulphate and glucosamine in combination for the formulation April 13 - 10:28 pm
• THF to wash column daily ? May 10 - 05:16 pm
• Ion pair chromatography May 26 - 07:23 pm
• Effect of Particle diameter on phase ratio February 5 - 01:46 am
• About flow rate and column i.d. February 7 - 05:13 am
• What is the effect of presssure drop on peaks? June 18 - 07:51 am
• What is the logic behid the RSD FOR THE ASSAY DETERMINATION.and sequential injection of std and samples. July 16 - 12:24 pm
• PE 900 series interface box? April 13 - 02:49 pm
• Asking about the packing material of Bilepack II June 2 - 12:47 am
• Autosampler from CTC-Analytics June 26 - 12:30 pm
• CombiPAL autosampler January 16 - 06:51 am
• How long the run time? December 30 - 10:33 pm
• Tissue Hydrolysis - AA analysis July 17 - 06:17 am
• Monolithic Column July 10 - 01:18 pm
• Starting analysis of peptides and proteins July 8 - 11:41 am
• Frits versus sieves (screen) July 12 - 08:01 pm
• pKa values July 19 - 03:46 pm
• HELP!! High column back pressure July 2 - 12:07 am
• Merck Chromolith Columns July 5 - 10:50 am
• Is it possible to analyse nateglinide and metformin in combination July 1 - 01:26 pm
• Flow halved, peak doubled ? July 4 - 02:37 am
• List of electrochemical compounds with voltage applied info? July 7 - 06:31 am
• Methionine oxidation July 7 - 03:08 pm
• Decontamination of a HPLC System from pencillin spores July 3 - 09:43 am
• Agilent 1100 Series VW Detector (Model G1314A) July 3 - 02:47 pm
• Durability of organic HPLC eluents July 6 - 09:01 am
• Method Validation at the QC Site??? December 30 - 10:44 pm
• Distorted peaks July 16 - 09:31 am
• Calibration curve reproducibility problem July 12 - 07:46 am
• Pressure drop July 14 - 05:45 am
• Neo-Heliopan Hydro in sunscreen products July 10 - 04:03 pm
• Waters 490 Uv-vis any good? July 12 - 11:16 am
• Using bovine serum albimin July 12 - 11:22 pm
• Electrochemical detection problem July 24 - 10:29 am
• Mystery Peak July 13 - 01:27 pm
• Negative Peak Problem July 19 - 07:30 pm
• Gentamicin sulphate July 31 - 03:09 pm
• Semie preparative LC July 16 - 07:00 am
• Holmium filters source July 18 - 06:33 am
• Preparative HPLC books July 16 - 07:15 am
• Cation Choice in PO4 Buffer Prep July 30 - 06:24 am
• Monitoring wavelengths/interferences July 17 - 07:41 am
• Agilent 1100 'Gameboy' February 12 - 01:36 am
• Non-zero y-intercept for Linearity of HPLC Assay July 23 - 05:12 am
• Windows driver for HP-IB 82335 IEEE-488 interface card. July 17 - 12:13 pm
• Problem with using Waters Millennium 32 Chromatography Manager. July 19 - 04:38 pm
• Baseline Noise July 18 - 03:33 pm
• Liposome analysis for phospholipid content using HPLC/ELSD? July 19 - 11:26 pm
• Peak area differences with different columns, but identical method and system? July 24 - 10:30 am
• Do I need a analog to digital converter for collecting data by serial port? July 20 - 12:11 pm
• Rejection of level in calibration range for plasma analysis July 20 - 03:23 am
• Isocratic Hold June 23 - 05:41 am
• Help for practical work of capillary electrophoresis November 22 - 03:11 am
• Ammonium bicarbonate for high pH gradient application July 25 - 08:23 pm
• I made a mistake in my method and ran air through my column all weekend at 40C; is there anyway that i can save the column?? July 24 - 11:14 am
• What do you guys think of Shimadzu LC's?? August 21 - 11:51 pm
• Borate buffer July 31 - 03:33 pm
• Tailing Factor Optimization for Sulfonates July 30 - 12:38 pm
• Pharmaceutical QC Question July 6 - 07:28 pm
• Chloro/Fluorosilicons November 29 - 01:31 pm
• Separation of angiotensins December 4 - 01:06 am
• Separation of closely related peptides (angiotensins) and the use of TEAF December 4 - 08:29 pm
• Counter current chromatography July 17 - 10:50 pm
• Uronic acids and monosaccharides August 22 - 01:11 pm
• Cation Exchange Column August 26 - 07:14 pm
• Amoperometric Detection of Carbohydrates August 22 - 01:59 pm
• Sulfobutylether-beta-cyclodextrin August 22 - 02:21 pm
• N-hyroxymorpholine July 2 - 01:15 pm
• Project Assurance Function under Prince 2 methodology of Project management July 3 - 01:11 am
• Signal drift in analysis of glycols August 22 - 04:15 pm
• Solid phase extraction July 13 - 08:39 am
• Isolation of natural compounds by HPLC July 13 - 12:39 pm
• Ammonium Hydroxide buffer? August 4 - 11:08 am
• HPLC Accuracy and Precision August 6 - 09:14 am
• Dioctyl sulfosuccinate sodium by HPLC? August 2 - 09:59 am
• Stability of Torsemide and Furosemide July 31 - 05:16 am
• Small Scale HPLC - - Why? How? August 9 - 03:39 am
• Air pumped on column August 8 - 11:18 pm
• Ketotifen - methods available? August 4 - 07:12 am
• Strange baseline August 31 - 12:32 am
• Octyl Methoxycinnamate by HPLC? July 5 - 05:23 pm
• Cleaning polystyrene-divinylbenzene GPC columns August 29 - 12:59 am
• Triamterene and HCTZ August 16 - 06:25 am
• Reverse flow increases resolution May 6 - 09:45 am
• Buffer preparation August 19 - 11:43 pm
• Factorial design in HPLC analysis August 20 - 09:55 pm
• Chemical database with informations on how to synthetize them August 21 - 07:33 am
• Impurity percent changes using diffrent HPLCs! August 29 - 01:00 am
• Where to look for existing methods? August 29 - 12:33 am
• Improper AIA conversion August 28 - 08:52 pm
• DNA purication!!! August 28 - 09:05 pm
• Purity analyses August 31 - 10:18 am
• How to set up limits for known impurities not listed in USP December 25 - 02:31 am
• PH of mobile phase & its effect on retention November 20 - 09:58 pm
• Beta-lactams by HPLC November 16 - 07:17 am
• Counter current chromatography November 16 - 08:06 am
• Change in chromatography by switching to a premix mobile phase on a gradiant December 5 - 08:18 am
• Regulatory Requirement for LC method November 20 - 10:09 pm
• Isomeric Separation Using LC September 3 - 01:04 pm
• Isoflavones in soy preparations October 31 - 07:01 am
• Normal-phase HPLC Handbook September 7 - 09:14 am
• Preparative scale chromatographie November 19 - 09:28 am
• Oleic Acid Assay??? September 5 - 02:30 pm
• Unknown Peak September 10 - 08:31 am
• Carbohydrate analysis September 8 - 06:57 am
• How to increase sensitivity? September 10 - 02:57 am
• Non-polar lipids with HPLC-ELSD September 5 - 02:00 pm
• Elution strength of sulfurous mobile phases September 4 - 07:43 am
• Ubidecarenone (Coenzyme Q10) September 6 - 04:45 am
• Bio- Rad 1350 and ValueChrom instrument interface problems September 6 - 08:04 am
• Gel Manufacturers for CGE September 6 - 08:42 am
• FPLC Valve September 6 - 06:40 pm
• Linearity & Tailing Factor September 7 - 08:23 pm
• Recovery does not correspond to Linearity data September 11 - 06:41 am
• Method Development & Method Validation November 28 - 06:07 am
• What is the Typical Concentration for Impurity Profile Analysis September 10 - 09:55 am
• Chromatographic purity / Related substances methods December 14 - 12:37 am
• Mobile Phase preparation: "Dial a Mix" October 23 - 04:06 am
• Quaternary ammonium chlorides December 6 - 07:47 am
• Phenethylamine by HPLC September 11 - 01:37 pm
• Spherical or irregular silica for LC column October 22 - 04:36 pm
• Carbohydrate analysis October 25 - 08:43 am
• ECD METHOD DEVELOPMENT October 25 - 08:36 am
• Clopidogeral hplc method October 25 - 12:07 am
• Probleam regarding amino column October 26 - 02:54 pm
• Cleaning LC syringes/needles to decrease contamination December 26 - 10:54 am
• Ion pairing reagent levels October 30 - 05:33 pm
• Radioactive contaimination in HPLC from use of FSA October 29 - 06:32 pm
• Decreased sensitivity November 6 - 11:59 pm
• 6-methyl-thiouracil October 30 - 06:43 am
• MULTI-DIMENSIONAL CHROMATOGRAPHY November 2 - 05:45 am
• Polysorbate 80 -post column derivitization November 1 - 08:01 am
• Active adhering to LC column November 1 - 04:14 pm
• Contamination in autosampler. November 2 - 05:05 am
• From TFA to Formic acid November 2 - 04:51 pm
• Precipitation of plasma proteins. Organic vs acid? November 5 - 02:47 pm
• Reporting results for impurities November 7 - 06:47 am
• Baseline drift during a gradient run November 7 - 11:33 pm
• Nano-LC November 28 - 08:05 am
• Separation of ethyl lactate(enantiomers) November 8 - 07:45 pm
• Mysterious peak in D-Lactate standards, separation of D and L Lactate? November 12 - 09:08 am
• Sucrose assay November 15 - 06:41 am
• Analyse of plant xanthophylls November 16 - 06:01 am
• Thiomersal HPLC analysis November 9 - 02:25 pm
• Selectivity and Specificity/Dynamic Range and Linearity Range November 10 - 12:00 pm
• PDA Detectors November 13 - 12:41 am
• Hplc-column information? November 12 - 12:08 pm
• Column probleam November 18 - 01:09 pm
• Chromatography data systems December 1 - 06:51 am
• DMF in Rp mobile phases November 18 - 01:12 pm
• Column Storage November 21 - 12:46 pm
• Mobile phase with basic pH September 1 - 06:35 pm
• Hitatchi amino acid analyser November 15 - 08:56 am
• Amine Modifiers and Cyano Columns November 18 - 01:22 pm
• RP-HPLC Columns with Embedded Polar Functional Groups November 20 - 08:56 am
• Immunoaffinity elution problems November 16 - 03:06 am
• Sample Cleanup by solid phase extraction November 20 - 04:49 pm
• Baseline of MRM Detection November 16 - 12:48 pm
• LD / LQ of impurities November 17 - 09:51 pm
• HPLC method for abietic acid and esters April 2 - 03:19 am
• Analyzing Sulfite in Proteins December 6 - 08:03 am
• Effects of PEG300 in 5% dextrose on the chromatography December 3 - 12:26 pm
• Large scale separation of small peptides by HPLC November 21 - 08:50 am
• Urine Sample Collection for Drug Analysis December 17 - 08:26 am
• Negative peak in Normal Phase HPLC November 28 - 12:08 am
• HPLC Analysis of Dimethyl Sulfoxide November 28 - 10:52 am
• Regulatory forums for HPLC November 28 - 02:44 pm
• Hypercarb coloumn and Nonyl/Octylphenole plus NPEO´s December 19 - 11:28 pm
• How to determine the purity percent of pesticide active ingridients November 29 - 06:14 pm
• Sample prep December 1 - 05:05 pm
• Chito oligosaccharides December 5 - 07:12 am
• Drug Peak in Blanks June 9 - 05:55 am
• Uranium estimation by Ion Chromatography December 13 - 12:07 pm
• Calculation of relative response factors of Impurities in HPLC December 6 - 02:17 am
• Chiral AGP column? December 8 - 10:18 am
• Amine Modifier Concentration December 6 - 10:10 am
• Need HPLC December 6 - 10:42 am
• Method Development using ChromSword December 19 - 04:09 pm
• Difficult Elution from SP-207 Resin December 8 - 04:41 am
• Increased Capacity with methanol December 8 - 05:11 am
• COMPUTER RELATED SYSTEM VALIDATION MATERIAL December 10 - 10:18 am
• RT's not constant when using a gradient December 14 - 07:29 am
• Cysteine & Methionine Seperation December 26 - 04:31 pm
• Ion pair chromatography December 18 - 06:58 am
• Weight of Calibration Curves December 26 - 05:37 am
• LC assay for alphaKG and/or succinate December 21 - 02:12 am
• Where can I find specifications for acceptable amount of Penicillin contamination? December 19 - 09:54 am
• Differences in resolution December 31 - 07:27 am
• Negative Peaks for RP-HPLC December 27 - 03:56 am
• Who can help me?a problem about the filter plate for hplc December 21 - 10:09 pm
• Laser induced Fluorescencedetection December 28 - 07:26 am
• How to analysis synthesis colorant in food December 30 - 12:47 am
• Any body can suggest HPLC METHOD OF ANALYSIS FOR ERYTHROMYCINE CYCLO-11, 12-CARBONIUM BULK AND TABLETS January 20 - 01:33 am
• Has anyone created a cleaning validation? January 7 - 06:32 am
• Is it retention time depends on concentraion January 5 - 07:05 pm
• Diastereomeric peaks in blank injection January 10 - 02:58 pm
• Ghost peaks in isocratic reversed phase chromatography June 13 - 10:45 pm
• Normal phase HPLC method validation January 12 - 02:36 am
• Reverse phase protocol for the HPLC analysis of fatty acids January 14 - 10:18 am
• Resolution January 8 - 11:33 am
• What is the effect of concentration on retention time January 9 - 03:51 am
• Tsk-gel column with high preasure January 18 - 01:57 am
• Hplc of sulphonated dyes January 11 - 10:08 am
• Preparative LC Information January 16 - 08:27 am
• Calibration Curve in different biological matrices January 13 - 03:26 am
• Pre-Columns and Guard Columns, When Needed? January 22 - 07:35 am
• Search for allicin standard January 20 - 11:44 pm
• NO BODYIS LOOKING AS MY ERYTHROMYCINE CARBONICUM QUESTION PLZ ANSWER THIS January 26 - 01:23 am
• Negative peak January 22 - 06:44 pm
• Negative peak in solvent January 17 - 06:31 am
• Pump problem caused by high pressure?? January 18 - 10:39 am
• Deproteination By Membrane Filtration January 16 - 11:45 pm
• Glucosamine and Ibuprofen HPLC method January 17 - 07:23 am
• Calibration for suppressed anion chromatography using a carbonate eluent January 22 - 02:56 pm
• Amino Acid Analysis January 31 - 06:48 am
• How Many HPLC's Sold Per Year? January 21 - 05:11 am
• Easy HPLC pump question January 22 - 09:44 pm
• Ultrasounds and hplc columns January 22 - 03:21 pm
• Error in Reporting of related substances test January 22 - 06:28 pm
• How to make .cdf files with ChemStation ? January 22 - 02:19 pm
• Differences among tetrabutylammonium salts July 2 - 07:20 am
• Difference between a pre-column and a guard column January 25 - 11:12 am
• Negative peaks using tetrabutylammonium August 22 - 07:31 am
• Column flushing January 28 - 02:36 am
• The effect of Methanol and silica January 25 - 08:33 am
• Fosfomycin analysis January 25 - 07:20 pm
• Updating January 30 - 06:36 am
• Wavelength maximum for assay January 31 - 01:19 am
• HP 1100 problem January 30 - 06:29 am
• Ghost peak January 30 - 03:53 pm
• Baseline problems with Agilent 1100 series RI detector January 31 - 06:02 pm
• How to reduce the emulsion after LLE? September 12 - 05:46 pm
• PEAK PURITY ANALYSIS USING WATERS 996 PDA July 28 - 12:40 pm
• HPLC change August 1 - 11:57 am
• HIGH PRESSURE.... July 8 - 07:39 am
• Zorbax SB-phenyl column_not easy to work with April 2 - 11:08 am
• Neutralization of gentamicin sulphate May 21 - 11:21 am
• Streptomycin in Milk August 14 - 08:04 am
• Maleic acid derivatives HPLC March 31 - 03:13 am
• HPLC sunscreen assay April 15 - 06:06 pm
• Ciral analysis of clemastine. July 15 - 06:12 pm
• Separation problem December 3 - 03:51 am
• Drifting baseline in gradient LC with acetonitrile mobile phase April 1 - 12:00 pm
• I need suggestions for a new column! July 30 - 12:38 pm
• TLC Solvent System October 30 - 05:32 am
• Now here's a tough separation August 22 - 04:38 pm
• Chiral Column Library November 8 - 10:43 am
• Retention Factor November 13 - 08:21 am
• Dionex Summit December 4 - 12:44 pm
• Chlorprothixene: Need a sensitive method December 10 - 01:32 am
• Manual or Automatic Injector? January 11 - 11:35 am
• Fraction Collector January 22 - 01:30 pm
• Tubochrom for workstation July 16 - 01:11 pm
• Capillary window breaking problem November 22 - 01:55 am
• Anyone got a good method for examining porphyrins by HPLC? January 4 - 01:24 pm
• Analytical method development May 27 - 08:18 am
• Assay Cross Validation January 27 - 10:44 am
• Chromatography software January 27 - 10:59 am
• Forced degradation/stress study June 23 - 07:28 am
• CLC January 2 - 09:18 am
• Peak shape bad June 9 - 07:06 am
• Which is useful, a quaternary pump or a binary pump? January 9 - 07:50 am
• HPLC Calibration April 12 - 08:12 am
• S/N ratio January 27 - 12:05 pm
• Column failures sources January 7 - 05:58 am
• Column Heaters January 7 - 05:50 am
• Change in RTs with different columns of the same material and same batch January 4 - 03:54 pm
• Columns July 26 - 05:09 am
• Ruthenium by ion chromatography April 12 - 03:33 pm
• Ethanolamine by IC June 25 - 10:29 pm
• Anyone online who want to help me about simple RP/equiqments"problems March 21 - 01:01 am
• Isocratic Methods: Pre-Mix Mobile Phase vs On-Line Mixing March 20 - 05:47 am
• Phase Collapse March 4 - 12:39 pm
• Problem with mobilphase February 21 - 08:55 am
• Carryover March 17 - 06:33 am
• Method developemnt for peptides of 4 amino acids difference February 21 - 08:35 am
• Response change February 15 - 03:16 pm
• Standard curve variability March 30 - 08:32 pm
• Columns for basic pharmaceuticals March 4 - 01:41 pm
• Mobile phase pH February 28 - 09:47 am
• Isomer separation February 28 - 09:56 am
• Buffer precipitation in HPLC February 28 - 09:58 am
• Buffers and volatility April 16 - 05:30 pm
• How to find the appropriate void marker? February 15 - 07:59 am
• Chemiluminescence detector used for HPLC detection February 21 - 02:05 pm
• LC Separation and Detection of alklyamines February 12 - 05:51 pm
• Computer assisted chromatography February 19 - 03:39 pm
• Kromasil February 4 - 06:16 am
• Aspirin November 5 - 07:32 am
• Band broadening February 7 - 01:15 am
• Used Equipment Advice? Suppliers? March 29 - 09:22 am
• SMB chromatography April 18 - 07:55 am
• RETAINING POLAR COMPOUNDS February 12 - 08:29 am
• Low pressure February 4 - 01:48 pm
• Acid-base indicators May 1 - 09:03 pm
• Study of peptide degradation in serum by HPLC February 5 - 03:52 pm
• Free fatty acid analysis in the presence of lipids February 6 - 12:52 am
• SEC/GPC for polylactic acid February 6 - 12:24 am
• Clarithromycin Impurities February 6 - 04:48 am
• How do organic/buffer affect pKa? April 18 - 09:03 pm
• Hydrophilic Interaction Chromatography February 12 - 01:58 am
• Retention Time variability in gradient run February 11 - 04:19 am
• Fast Flow and Small Columns: Am I turbulent? February 10 - 06:19 pm
• Establishing specificity using HPLC methods for excipients June 11 - 04:43 am
• Coverting % area to %w/w February 15 - 07:27 am
• Gradient vs isocratic chromatography February 21 - 10:34 am
• Column diameter February 19 - 10:29 am
• Reason for negative peaks in RP HPLC? February 21 - 11:15 pm
• HPLC Sample Vials March 1 - 03:48 pm
• Protein binds in a weak but not in a strong ion exchanger March 11 - 09:09 am
• Increasing baseline drift for samples with little chromaphore February 20 - 01:53 pm
• High Pressure Problems February 21 - 08:48 am
• Separation of nucleosides, nucleotides and nitrogen bases October 13 - 08:01 am
• Portable HPLC May 29 - 02:12 pm
• Solanidine February 25 - 07:57 am
• Analytical supercritical fluid chromatography (SFC) February 27 - 02:03 am
• Problem with absorbtion coefficients March 8 - 12:13 am
• Lower detector intensity February 28 - 10:23 am
• To analyze Diketones in HPLC March 11 - 09:02 am
• Hi pH stable HPLC columns April 17 - 05:45 pm
• Problems with Heptane? March 20 - 11:19 am
• Advice on software to handle chromatogram? April 3 - 05:56 am
• Polydimethylsiloxane (PDMS) using ELSD March 13 - 05:29 am
• Protein purification ligands supplier March 8 - 12:49 am
• Petrolatum March 8 - 07:23 am
• Fronting and "foot shaped" signals March 15 - 02:21 pm
• Determination of lactic acid by HPLC June 18 - 12:56 am
• Phospholipids analysis in chocolate May 21 - 02:44 pm
• Reduction of run time March 13 - 12:22 pm
• Passivation HPLC stainless steel March 20 - 04:49 am
• Column aging? March 27 - 11:12 am
• What a column and mobile phase should be used? March 18 - 03:13 pm
• HPLC quantitative analysis March 27 - 11:18 am
• Injection volume and concentration March 18 - 03:33 pm
• Waters development kit March 19 - 01:07 pm
• Caffeine by LC March 21 - 03:24 pm
• Calibration in Chemstation March 25 - 06:46 am
• PH 5.0 buffer for DEAE SEPHACEL?????????? March 25 - 03:06 pm
• Find the analysis method of N-Phenylmaleimide?[941-69-5] !!!! March 21 - 10:32 pm
• Non-identical retention times March 25 - 03:42 am
• Agilent, Waters and cross control of same (by them or anyone else) March 27 - 11:21 am
• Organo metallics April 15 - 05:32 pm
• Column Void Volume April 1 - 07:06 am
• Size exclusion cartridges for sample preparation March 27 - 06:04 am
• Hydrolysis of urethanes March 25 - 07:50 pm
• Fluorescent marker April 1 - 01:51 pm
• Problem of separation between aromatic polar compounds June 10 - 08:42 pm
• Cappilar HPLC April 1 - 09:27 am
• FDA requirement for pH of the Mobile Phase April 8 - 11:37 pm
• Long equilibration time abd very broad peak on Xterra C18 May 2 - 07:57 am
• Cyano and phenyl columns switch from reversed to normal phase April 11 - 08:55 pm
• Sticky molecules. How to deal with them? April 8 - 11:55 pm
• Base deactivated columns. April 4 - 07:56 am
• Sticky molecules. Adsorption or decomposition. April 15 - 06:27 pm
• Column of diastereomer separation? April 5 - 01:58 am
• Dalton units April 9 - 11:56 pm
• 3-Chloroperbenzoic Acid [937-14-4] March 25 - 11:11 am
• Asymmetry Question April 10 - 10:01 pm
• Calibration Curve April 22 - 05:06 am
• Oxcarbazepine by hplc May 2 - 11:38 pm
• Unknown Peak probably from gradient system May 23 - 08:09 am
• Chloride Peak Tailing/Asymmetry April 18 - 02:17 pm
• N-octadecylphosphonic acid ?? April 19 - 08:20 am
• HPLC Miniaturization April 12 - 08:03 am
• Use of graphite columns April 12 - 06:27 pm
• Column Heaters April 17 - 07:41 am
• Ion Suppressor April 22 - 11:44 am
• Resolution, gradient, HP Vs Waters 2690 April 23 - 06:44 am
• Re-validation of assay method April 20 - 12:37 pm
• Stress study April 18 - 08:52 am
• FPLC ?? August 5 - 11:56 am
• Dissolved oxygen and electrochemical detection April 22 - 02:34 pm
• Acid Interaction May 17 - 03:28 am
• Retention time drifting with ion-pair chromatography May 22 - 01:07 am
• RENTIVITY PROBLEAM April 22 - 06:05 am
• Inconsistent SPE results May 10 - 03:12 pm
• Calibration curve II: to weight or not to weight April 26 - 05:20 am
• How to isolate Vitamin E and Carotene using Chromatography April 23 - 08:55 am
• Relative Response Factor May 10 - 02:57 pm
• Is there any way to put Millinium results into Excel correctly? April 25 - 07:18 am
• Buffers April 30 - 11:24 am
• HPLC make up April 26 - 12:02 pm
• HPLC April 23 - 10:55 pm
• To remove CH2Cl2-originated peaks ? May 2 - 01:38 am
• Microbore LC Columns May 1 - 10:51 am
• Which columns are best for isomer separation May 23 - 10:56 pm
• Carbohydrate analysis - split peaks May 2 - 05:31 am
• I need a method to determine fatty acids with RPLC May 6 - 01:41 pm
• Any opinions about HPLC Troubleshooting courses? May 8 - 10:17 am
• Mechanical resistance of silica ... May 4 - 08:50 am
• HPLC of sugar phosphates May 2 - 06:02 am
• Dansyl Chloride Deriv. for Amines May 3 - 08:45 am
• Water on Column -- Problem? May 3 - 12:17 am
• Instrument logbooks May 6 - 11:34 am
• HIGH SPEED HPLC MICROBORE COLUMNS May 7 - 06:53 am
• How to make PEAKS ATENUATION ? May 6 - 07:39 am
• Waters 2690 degasser module May 13 - 01:13 am
• Ask help for Analysis of Alanine and Ammonium May 7 - 12:30 am
• Inorganics on HPLC May 9 - 08:13 am
• PKa logP softwares April 25 - 11:57 pm
• Connect a integrator to a PC April 19 - 10:24 am
• Anyone has good experience with ELS? June 29 - 06:07 am
• ASSASY OF COMPOUND HAVING POLYMORPHS May 13 - 07:00 pm
• Tom please check out my previous talk on isomer separation May 4 - 08:26 am
• Detection of microgram quantities of 3-dimethyl-1-phenyl-2-butanone May 5 - 06:12 pm
• Ways to prepare a buffer? May 13 - 01:37 am
• Waters Alliance & Millennium32 Problem May 8 - 05:52 am
• Separation of polar isomers by reversed phase HPLC May 17 - 05:18 am
• Calculating peak area on basis of the absorption coefficient May 16 - 12:49 am
• Shimadzu equipment May 8 - 04:57 pm
• Limit of Detection (LOD)/Limit of Quantitation (LOQ) May 9 - 10:45 am
• HPLC Column choice May 12 - 07:22 pm
• How to determine the consentration of creatinine in urine? May 30 - 12:10 pm
• ESI sensitivity and mobile phase May 9 - 03:14 pm
• CHOICE OF DIODE ARRAY DETECTORS May 9 - 02:34 pm
• How to recognize effects of residual metals May 18 - 03:49 pm
• Liranaftate HPLC method May 12 - 07:25 pm
• Postcolumn reaction coil May 14 - 04:11 am
• Separation of glucose and sodium chloride May 30 - 04:26 am
• ABS May 21 - 05:09 am
• Supplier suggestion for solid HPLC vial caps needed May 14 - 05:11 am
• Accuracy May 14 - 01:31 am
• Column bubbles May 17 - 01:43 am
• Difference on PDA detectors from different manufacturers May 22 - 06:47 am
• CN or C5 columns stable at pH > 9 May 18 - 03:57 pm
• Disappearing protein May 17 - 03:22 am
• ChromSword software May 20 - 02:00 pm
• Method for Glycerol (~20%)in Skin Cream? June 19 - 09:34 pm
• Whither HPLC? May 27 - 03:52 am
• Agilent 1050/1100 Chemstation software glitches May 21 - 10:24 am
• Model for ELSD May 21 - 07:23 pm
• Linear diastereomer separation May 23 - 05:19 pm
• Reducing column dimension June 5 - 08:46 am
• Assaying Sodium Metabisulphite by HPLC May 24 - 10:18 am
• Agilent ChemStation USP system suit calculations May 29 - 01:33 am
• Degassing May 23 - 09:14 pm
• Sample carryover associated with autosampler June 4 - 02:06 pm
• Hp Chemstation Software(G2180AA) for HP 3D CE system? May 24 - 09:29 am
• HPLC Method Development April 26 - 07:05 am
• Contamination from caps May 27 - 06:28 pm
• Quaternary ammonium salts by HPLC June 3 - 08:30 am
• Sensitivity criteria for PDA-detector May 26 - 04:48 pm
• Deggasing : Helium or vacum membranes May 29 - 03:13 pm
• CE handbook May 28 - 09:57 am
• Problem Negative peak in RI detection June 11 - 12:00 am
• Agarose Bead Dissociation May 29 - 11:31 am
• Solubility of 25mM phosphate at pH 5.5 mixed with 30% methanol and 10% THF June 2 - 02:31 pm
• Buffers in mobile phase June 2 - 02:34 pm
• Method to Measure THP acid in THP anhydride June 1 - 04:32 am
• Calculating peak area via absorption coefficient May 7 - 07:25 am
• Cefixime June 1 - 05:23 am
• GLP/GMP enviroment June 5 - 06:43 am
• Number of significant figures to use June 5 - 11:27 am
• Organic modifier strength June 11 - 01:32 pm
• HPLC mobile phase life June 7 - 06:12 am
• Method Validation June 7 - 08:36 am
• Analysis of Methyl and Propyl Paraben in Shampoo June 7 - 02:00 pm
• Thc removal from hemp oil June 18 - 12:01 am
• UV cutoff table June 10 - 02:28 pm
• HPLC column June 9 - 07:18 am
• HPLC column filing cabinets June 7 - 11:03 am
• Chiral separations June 13 - 08:47 am
• Collapse of HPLC column June 29 - 05:53 am
• Cr-picolinate hplc method of analysis June 21 - 10:19 am
• PEO on TSK gel June 18 - 11:41 pm
• USP Heavy metals June 12 - 05:24 am
• Internal standard June 19 - 06:01 am
• Will flow rate affect the sensitivity? June 13 - 11:16 pm
• HPLC method for determining solubility of organic compounds June 13 - 04:02 am
• Millennium32 version 4.0 June 13 - 02:19 am
• Relationship between column i.d. and injection volume June 14 - 07:59 pm
• HPLC PEAK PURITY June 30 - 12:41 am
• Placebo solubility June 19 - 08:18 am
• Glycophosphate? June 19 - 10:26 am
• What is liqiud scintilation? June 19 - 10:42 am
• HILIC June 24 - 02:57 pm
• Empty PEEK columns Suppliers June 21 - 12:15 am
• Moisture content in bonded hydrated molecules June 24 - 10:45 pm
• HILIC SPE cartridges June 25 - 07:39 pm
• Guard column validation June 25 - 09:14 am
• Formol titrations June 26 - 10:34 pm
• Retention Time Shifts June 30 - 06:32 pm
• Capillary 2D liquid chromatography June 5 - 08:31 am
• Peptide separation and biological activity June 28 - 08:11 am
• Radio Detector for HPLC February 28 - 08:54 am
• John W. Dolan's "LC Troubleshooting" predictions (latest LCGC NA, 20/3, 2002) March 19 - 08:29 am
• Pneumo-hydraulic hplc pump wanted April 24 - 10:35 am
• ASCII to AIA file conversion March 1 - 02:02 pm
• JMBS March 19 - 04:42 pm
• PH 1.00 buffer for calibration April 13 - 02:52 pm
• Urgent! May 15 - 09:48 am
• Wanted: supplier(s) of empty plastic chromatography cartridges. June 21 - 07:55 am
• Glass tubes/caps May 3 - 02:21 pm
• How a drift baseline affects integration July 22 - 06:53 am
• Buying An HPLC (for newbies) July 16 - 02:34 am
• Chromatography Software May 28 - 09:41 pm
• Improving post-column reaction Flourescence reproducability July 25 - 12:23 pm
• HPLC Column Log system for the Lab July 20 - 05:10 am
• Low pressure mixing - TFA Buffers July 17 - 02:34 pm
• Use of n-Propanol in RP-HPLC mobile phases! July 3 - 06:17 am
• Input signal out of range July 24 - 11:51 am
• Columns for underivatized amino acids analysis April 10 - 03:02 am
• DNA analysis by RP-HPLC August 26 - 03:29 pm
• UV_VIS Detector flowcell July 3 - 01:49 pm
• Bandwidth in HPLC detector May 11 - 07:56 pm
• MeOH vs MeCN? July 10 - 11:09 pm
• Mass Balance requirements for method validation July 2 - 12:25 am
• Any HPLC manufacturers based in Spain? July 4 - 12:03 pm
• Networked Chemstation July 20 - 01:46 pm
• Column Log July 9 - 07:43 pm
• Two different lines from the pump system July 1 - 07:41 am
• Peak splitting problem July 21 - 01:52 pm
• Signal to Noise July 3 - 05:04 am
• Quantification of TRICLOSAN in personal care products June 26 - 07:04 am
• SOP-General criteria for the suitable application of HPLC methods July 2 - 08:56 am
• Method Transfer July 2 - 03:17 am
• Chiral Column for Levofloxacin July 2 - 03:49 am
• 21CFR Part 11 template July 20 - 02:02 pm
• Polysiloxane-based anti-foaming agents in beverages July 10 - 01:15 am
• Peak to valley ratio for resolution July 3 - 11:45 am
• Suppliers for polymer-based weak cation exchange SPE cartridges July 5 - 11:11 am
• Suitability test before and after the analysis July 9 - 07:29 pm
• Min pressure setpoint for pumps July 11 - 05:44 pm
• Stability Analysis of Dexamethasone with RP-HPLC July 14 - 03:43 am
• HP1100 UV-detector at 200nm -> collapsed response after changing lamps July 16 - 08:24 am
• Unknown Peak in LC system (UV detection) July 23 - 05:37 am
• Please help with my impurity linearity July 15 - 08:37 pm
• Doxazosin Assay, Dear Friends July 25 - 09:12 am
• 75 um ID columns July 15 - 08:55 pm
• APROPIATE BUFFER July 18 - 03:22 am
• How to prepare 20% methanol in water? July 17 - 06:48 am
• Column clean up help!!!!! August 8 - 03:02 pm
• Six Sigma and Analytical Testing July 21 - 08:46 am
• L53 Columns July 14 - 03:52 am
• Injection Accuracy July 16 - 02:42 pm
• HPLC-fluorimetric detector-time zero July 16 - 02:34 am
• GPC Mobile Phase Modfiers July 16 - 05:14 pm
• Increase in RT when I increase temp July 17 - 11:35 pm
• SPE Manifold July 17 - 08:20 am
• Copper detection by ion chromatography? July 19 - 04:39 am
• Detection of imidazole by hplc April 2 - 04:41 am
• Cl in silica based slurry July 18 - 11:49 am
• C18 and octadecyldimethylsilyl July 24 - 02:49 am
• Haddad Transfer Equation July 18 - 05:25 am
• METHOD FOR VIT B12 April 30 - 12:03 pm
• Reloading of HPLC millennium software July 22 - 11:51 am
• Please help me solve my baseline problem July 25 - 01:10 pm
• Irregular peaks in the baseline during gradient August 24 - 06:31 am
• Estimation of a retention time when changing the mobile phase July 19 - 06:43 pm
• Method for determination of sugars and PEG by HPLC/IC July 22 - 12:49 am
• Method for DDBSA July 19 - 12:57 pm
• DRI detector and negative peak August 5 - 06:52 am
• Quantitative detail - standard injection (single or multiple?) August 8 - 05:11 pm
• THF compatible tubings July 29 - 03:12 am
• Polar dirt stuck in C18 column? July 29 - 06:46 am
• CuSO4 buffer usage July 27 - 08:07 am
• Improving Tailing Factor August 1 - 08:50 am
• Fixing no. of theoritical plates July 30 - 06:21 am
• PEG-8000 August 11 - 04:10 am
• HPLC EVALUATION August 21 - 08:57 am
• KH2PO4 BUFFER SOLUBILITY August 7 - 07:00 am
• Diol Column August 8 - 08:40 am
• Denaturing HPLC August 8 - 03:53 am
• Fast screening methods and monolithic columns August 5 - 11:11 am
• Hyaluronic Acid Analysis February 11 - 09:37 pm
• Vial septa crumbs from injector valve August 8 - 11:11 pm
• Beckman System Gold HPLC system August 12 - 09:44 am
• Theorical questions August 9 - 05:12 pm
• Analysis of Fmoc-Amino Acid August 9 - 01:29 pm
• RAM columns August 14 - 04:48 am
• How to separate L-cysteine from L-cystine for HPLC/UV analysis August 12 - 09:55 am
• Accuracy for a method when no Standards are Available August 15 - 09:38 am
• Area reproducibility August 16 - 05:22 am
• Cation Exchange column August 20 - 01:27 pm
• Maximum column temperature August 22 - 03:43 pm
• Biphosphonates August 17 - 10:18 am
• Where to lease a HPLC? August 19 - 08:06 am
• Flow splitter calculation August 22 - 03:50 pm
• RP HPLC METHOD OF ANALYSIS FOR SERRATIPAPTIDASE August 29 - 11:39 am
• Ultron ES-pepsin column August 20 - 12:22 pm
• Basic integration theory August 21 - 05:38 pm
• Peak Tailing .... August 24 - 12:22 pm
• System suitability test August 29 - 06:13 am
• Biotrap column August 30 - 10:47 am
• LC System Clean up August 29 - 10:49 am
• PH in RP Mobile Phases August 30 - 07:03 pm
• Looking for an old Paper August 21 - 05:16 am
• Preparative HPLC August 21 - 01:17 pm
• Help with normal phase vs reverse phase explanation August 5 - 09:33 am
• Petro industry application July 21 - 04:18 am
• Column rinsing in reverse direction August 31 - 07:13 am
• Morphine in injectable August 14 - 05:46 am
• Separation according to size August 16 - 03:21 am
• Void Volume Determinations August 20 - 02:05 pm
• Separation of NaCl January 20 - 10:15 pm
• Determination of cellulose in liquid products January 23 - 08:32 am
• Oligomeric Proanthocianidynes (OPC's) from Pine and Grape March 3 - 08:21 am
• HITACHI L9100 March 6 - 04:39 am
• Vial crusher March 8 - 07:45 am
• HPLC-colum? September 24 - 04:42 am
• What is a good antibacterial buffer additive. September 4 - 11:39 am
• Organic Acids by RP-HPLC June 14 - 02:25 pm
• Silanizing Glassware November 6 - 07:50 pm
• Mechanisms of Chromatographic separation December 27 - 10:48 am
• UV absorbance November 6 - 07:53 pm
• Stavudine impurities method September 19 - 12:13 am
• Hplc analysis of amino acids using uv-vis detector September 27 - 02:59 pm
• Void Volume November 6 - 07:53 pm
• Electrochemical detector December 20 - 06:44 pm
• HPLC-ECD- using ion pair reagents for catecholamines November 6 - 07:45 pm
• HPLC Performance December 19 - 08:57 am
• Laser Induced Fluorescence Detection November 7 - 05:51 am
• Mobile phase for lenthy analysis December 26 - 09:13 am
• LC Method November 6 - 07:56 pm
• Gradient to isocratic November 6 - 07:30 pm
• DETECTION OF AMINO ACIDS IN SMALL SAMPLE VOLUME AND fmol range October 22 - 04:29 pm
• THF containing mobile phase September 23 - 08:14 pm
• Clinical HPLC September 6 - 08:44 am
• Chiral Separation September 23 - 02:15 am
• DMSO on Reversed phase September 24 - 02:33 am
• Need an article, please help March 25 - 10:43 pm
• Need some guidelines June 5 - 12:41 am
• Negative peak problem in RI detector September 12 - 07:17 am
• Help DAD Detector Baseline September 5 - 08:37 am
• High pressure during automated set of runs leads to system shutdown November 29 - 01:30 am
• Peak purity vs. Assay value September 16 - 07:33 am
• Iminium Salt September 6 - 12:06 am
• Animal Feed sample preparation November 6 - 07:38 pm
• Licopene determination and its epoxides September 13 - 11:18 am
• Storage of THF - neat vs. mixed with ACN September 13 - 08:34 am
• Acrylamide April 7 - 04:10 am
• Water in Silica column February 26 - 06:52 am
• Lost peak in HPCEC September 16 - 08:33 am
• Calling the chromatography gods to settle an inter-lab dispute September 12 - 03:22 pm
• Storage of anion exchange column September 12 - 06:29 pm
• Prediction of retention from chemical structures September 14 - 02:15 am
• Glucosamine November 6 - 07:27 pm
• CMC in laundry powders November 6 - 07:23 pm
• Theories relating chemical structures with retention in RP-HPLC June 2 - 02:03 pm
• Use of pKa value September 26 - 09:15 am
• LC method for quaternary amines September 17 - 04:30 am
• New HPLC columns Qualification procedure September 24 - 05:31 am
• 21CFR Part 11 question September 24 - 02:59 pm
• RP column vs slurry packed cartridges September 17 - 09:59 am
• Affinity chromatography the hard way? September 24 - 04:18 am
• Elusion sequence September 19 - 12:08 pm
• Aspirin USP assay method? October 10 - 02:44 pm
• Analysis of peptides by HPLC using PDA detector September 20 - 12:11 am
• HPLC of mixture of HC and FA September 21 - 09:20 am
• Chiral isomer separation September 20 - 05:04 am
• Determination of EDTA Content in ppm or ppb levels September 24 - 09:35 am
• Polymorphs analysis by HPLC September 24 - 12:12 pm
• Peak area units for linearity graph April 28 - 06:03 am
• Dionex AND QC October 2 - 07:21 am
• Looking for a hplc column September 24 - 02:32 pm
• Standardize a standard? September 25 - 05:27 am
• Amino ethyl ethylene urea September 25 - 09:59 am
• Wavelength adjustment? October 2 - 07:40 am
• 2D chromatography run times September 29 - 02:26 pm
• Separation of mixture b-cyclodextrin complex/free drug September 26 - 05:40 am
• Inertsil Bioptic AV-2 September 26 - 04:06 pm
• Bromide ion identification October 4 - 01:05 pm
• Enantiomeric separations September 30 - 08:07 am
• Can silica induce changes to samples? October 1 - 11:51 pm
• Wavevy baseline October 3 - 08:15 am
• Peptide/Protein Deconvolution Software August 22 - 10:08 am
• HPLC methods for determining log P October 9 - 04:49 am
• Unidentified split peak question December 24 - 07:07 am
• Validation methedology for precision study October 10 - 06:31 am
• Changing response factors October 4 - 08:58 am
• Area counts changed with different flow rates October 7 - 02:47 am
• Standard curves October 11 - 07:46 am
• How to export the data from Star LC Workstation 5.0 into ASCII file October 7 - 05:38 am
• Rotational isomers in RP-C18 analysis October 9 - 11:02 pm
• Polar embedded phases for Nucleotide base/nucleoside analyses October 22 - 11:20 am
• How to calculate 95%Confidence curve? October 4 - 06:01 pm
• Triglycerides October 9 - 05:10 am
• Molecular Sizer? October 7 - 11:29 pm
• TLC October 5 - 03:01 pm
• Botox standard October 7 - 03:48 pm
• Guard Columns October 9 - 12:40 pm
• Paramertrs for validation of dissolution method. October 1 - 11:12 am
• Revalidation on tightening of the specification October 11 - 05:30 am
• Analysing Betaine content in beet molasses October 8 - 09:28 pm
• Cinitapride tablets / quantitation by HPLC / low recovery October 11 - 05:52 am
• Cross-validation October 9 - 10:02 am
• Polypropylene vs. glass vials October 10 - 02:15 am
• Pressure spikes October 9 - 11:32 pm
• HPLC purification of DNA oligos (35-mer) October 11 - 11:16 am
• Method Development vs. Method Validation October 14 - 11:01 am
• Evaporative Light Scattering Detectors November 7 - 05:25 am
• Unexplainable Wavy Baseline Without Flow January 30 - 03:52 pm
• Unexplainable problem with Specificity October 14 - 06:57 am
• Dichlorobenzoic Acid Isomer HPLC October 18 - 12:09 pm
• LOQ and LOD calculation October 14 - 09:01 pm
• Is ChemStore any good? October 28 - 10:12 am
• LC Radioactive detectors October 14 - 10:22 pm
• Could you said how to separate sugar fatty acid esters by chromatography? October 21 - 10:54 am
• 2-Phenoxyethanol October 18 - 10:46 am
• Hydroxyzine November 1 - 03:28 pm
• Dissolution Profiles - straw poll October 24 - 01:54 pm
• Related substances in Rocuronium bromude - looking for HPLC method October 21 - 10:11 am
• Variable retention time October 23 - 04:04 pm
• Amino Acid Separation October 23 - 06:50 am
• LIMS systems October 28 - 04:22 pm
• Help Trace Analysis October 21 - 02:16 pm
• Hplc method for Trimebutine maleate in tablets. October 21 - 10:47 am
• Acceptance Criteria for analytical Method Validation October 24 - 06:33 am
• Strange Spectra October 23 - 11:03 pm
• Niacin and lovastatin February 21 - 12:27 am
• System Suit- Detector on/off? October 30 - 02:01 pm
• Excipient Studies? October 24 - 01:10 pm
• Help about Impurities October 24 - 02:17 pm
• Removal of ftalates from Milli-Q water October 26 - 07:35 pm
• Peak symmetry factor for drifting baseline October 27 - 04:51 pm
• Method development language (MDL) for auotsampler October 24 - 10:10 am
• Shimadzu users: asking about SCL -10A controller October 27 - 05:50 pm
• Agilent 1100 and Firmware v 5.01 October 25 - 11:30 am
• Theoretical t0 - how is it derived? November 1 - 04:19 am
• Problem with Waters Empower/Millinium software November 27 - 01:26 am
• Chaotropic and Lyotropic agents October 31 - 03:02 am
• PDA detectors November 4 - 07:16 am
• Concentration dependant retention times? November 1 - 04:13 am
• Waters AMDS November 12 - 05:26 am
• HPLC problem with PAH's analysis after use of triethylamine buffer November 4 - 02:12 am
• Peak Purity on DAD-Detector October 30 - 11:47 am
• Buffers problem November 4 - 03:43 am
• Chemstation problem - disappearing files October 31 - 04:02 am
• Overloading November 1 - 11:45 am
• CN phase stability November 13 - 01:12 pm
• Sodium omadine assay November 1 - 09:00 am
• Diazolidinyl Urea (Germall2) November 1 - 06:10 pm
• Alendronate Sodium PF dissolution November 12 - 05:15 am
• Specific recovery question November 14 - 12:38 pm
• Selectivity verse Specificity November 5 - 07:59 am
• Have anyone an idea about TSK-Butyl? November 8 - 11:00 am
• 2 deoxy d glucose / glucose separation November 14 - 07:24 am
• 2-methyl-4-isothiazolin-3-one (MIT) November 8 - 12:33 pm
• Method for Acetylsalicylic Acid Tablets November 18 - 05:55 pm
• Acetic-, propionic-, butyric- valeric acid with HPLC April 14 - 04:48 am
• QTOF November 13 - 08:18 am
• HPLC mobile-phase November 18 - 06:12 pm
• Universal test for RP supports November 21 - 03:25 pm
• Sodium docusate as mobile phase November 27 - 10:32 pm
• Why do retention times of isomers correlate with their boiling points? November 15 - 06:44 am
• Problem with column dewetting April 25 - 11:18 am
• Recovery problem November 22 - 06:23 am
• Vitamins November 19 - 07:27 am
• Dual active assay ruggedness issue December 3 - 03:55 pm
• Fatty acids by GC or HPLC? November 20 - 07:09 am
• Hplc retest / failures June 5 - 09:08 pm
• NH4H2PO4 pKa November 27 - 11:53 am
• SFC check valve availability November 21 - 09:34 am
• A Persistent Ghost peak(need help) November 23 - 05:12 am
• Acetic acid tail in FFAP column November 24 - 08:49 am
• About pKa's, Acids and Bases March 27 - 07:18 am
• Tamoxifen stereoisomer separation November 25 - 10:40 am
• Permiation by liquids November 25 - 03:48 am
• Dead time and chaotropic modifiers December 12 - 07:29 am
• Can I use my Waters 625 LC system with a LC-318 Octadecyl column? November 28 - 12:57 pm
• EDC (or EDAC, Ethyl-3-(3-dimethylaminopropyl)-carbodiimide November 26 - 12:25 pm
• Relative retention times(RRT) fluctuation November 27 - 11:58 am
• Salicylic acid and betamethasone dipropionate November 27 - 02:43 am
• Routine system performance December 1 - 01:28 pm
• Choice of optimum extraction solvent December 7 - 07:41 am
• Any trick to derivatize and analyze monophospahte December 5 - 04:45 pm
• EP assay for Vitamin A December 5 - 03:08 am
• Adsorption in plastics December 12 - 02:16 am
• Which column? April 6 - 06:13 am
• Contract laboratory for Nitroso compounds analysis December 5 - 12:06 pm
• Cerity March 13 - 08:08 am
• Commercial source of human plasma for validation? December 10 - 07:06 am
• Bromopride December 6 - 08:01 am
• FDA 483 letters and reviews December 9 - 12:33 pm
• After flushing column with very big flow rate, what should I do next? December 10 - 05:35 am
• How to maintain HPLC column December 9 - 05:59 am
• Method for Bromopride Assay December 9 - 06:19 am
• Ion pair reproducibility December 17 - 05:44 pm
• HI 1200 upgrade for 7673 ALS August 13 - 08:07 am
• HPLC analytical method of azithromycin May 17 - 12:26 pm
• Bromopride Method December 9 - 09:58 am
• Prednisolone December 13 - 06:07 am
• Gilson Prep. Instrument December 10 - 05:18 pm
• Bioanalytical question December 12 - 08:27 am
• Information about capillary HPLC December 18 - 01:54 pm
• DIY Affinity columns December 20 - 02:07 am
• Qualification of columns December 18 - 06:27 am
• Ammonium Carbamate December 16 - 12:35 pm
• ActiveX Chart Component for AIA/netCDF files December 16 - 09:52 am
• Metal-chelater complex separation from original chelater in LC December 20 - 11:04 am
• Validation of USP methods December 27 - 01:27 am
• LC to "Dye" for! December 20 - 11:37 am
• Substantial decrase of benzo(a)pyrene peak in PAH analysis December 20 - 01:38 am
• PH of a compound December 24 - 02:46 pm
• Problem of Peak splitting December 29 - 07:32 am
• Waters Empower Software January 9 - 07:07 am
• HPLC software validation June 23 - 07:18 am
• Prednisolone - related substances December 1 - 05:04 am
• Peak "Fronting" November 17 - 06:09 pm
• AllTech Rocket Columns December 11 - 11:59 am
• Capillary Electrophoresis December 17 - 03:24 pm
• Niacin and lovastatin April 27 - 04:50 pm
• Sample treatment for determination for vitamins in HPLC June 22 - 01:26 am
• Problem with high preasure in a microbore column December 4 - 12:10 pm
• Looking for some schematics, hope someone can help September 21 - 01:00 am
• ION CHROMATOGRAPHY July 10 - 10:19 am
• Standard curve in catecholamine analysis March 3 - 04:53 pm
• Phenoxyethanol March 29 - 07:18 am
• Percent recovery of an analyte May 31 - 08:44 am
• Hydramethylnon April 29 - 11:10 am
• Tailing and Fronting May 19 - 01:04 pm
• Delay volume in HPLC July 29 - 04:37 pm
• KH2PO4 BUFFER SOLUBILITY August 14 - 04:30 am
• HPLC equipment. June 18 - 07:59 pm
• TLC September 26 - 05:48 pm
• Validation for antioxidant methods of analysis . September 14 - 03:21 pm
• Lincomycin October 23 - 11:45 am
• Common Lab Practice - Number of Injections November 21 - 04:26 pm
• Setting spec's for assymetry vs H/W ratios November 25 - 03:40 pm
• Spectroscopy Question! November 27 - 01:01 pm
• Runtime21 July 24 - 11:26 am
• New online electrophoresis forum August 22 - 07:17 pm
• HPLC Web Directory/Search August 8 - 09:23 am
• T.DBL August 8 - 11:56 pm
• Please help me January 20 - 04:39 am
• A plea... More information, please August 30 - 07:05 pm
• CE equipment September 15 - 10:53 pm
• Chromatography forum October 11 - 08:14 am
• Help me find it!!!! December 7 - 02:12 pm
• Humour and Analytical Chemistry December 23 - 05:07 am
• Denaturing HPLC for DNA analysis (Wave system) June 5 - 12:15 pm
• Diastereo isomers By HPLC June 18 - 08:52 am
• HPLC method of DONEPEZIL (ARICEPT) June 21 - 12:31 am
• Ask for some suggestions on capillary HPLC January 17 - 12:05 pm
• UPS for LC GC and other lab instruments July 14 - 11:46 am
• Specificity Question April 5 - 03:59 am
• Gradient methods July 30 - 05:12 am
• GLP vs non-GLP January 10 - 07:43 pm
• HPLC method for androsterone and related substances January 3 - 12:19 pm
• Desalting by HPLC January 9 - 01:13 am
• What can I use instead of Hexansulfonic acid Sodium salt ? January 6 - 10:50 am
• High Pressure versus Low Pressure Mixing January 11 - 07:15 am
• TLC Records January 8 - 01:21 am
• Low pressure gradient hplc vs high pressure gradient - advantages & disadvantagess January 9 - 08:04 am
• Column for substituted saccharids January 7 - 01:38 am
• ELSD detection repeatability June 3 - 01:15 am
• For fast analysis what column shell I buy, help needed January 13 - 12:41 am
• ...seperation of highly hydrophobic protected peptides... January 8 - 08:48 am
• Stabilization of a strong anion exchange column January 8 - 11:44 am
• HILIC column ok with TFA? January 14 - 04:17 pm
• Metformin +gliclazide hplc method of analysis May 6 - 08:13 pm
• Equivalency vs. Validation March 6 - 05:42 am
• Small particle-Large pore HPLC columns April 6 - 04:10 pm
• THEORETICAL PLATES January 22 - 03:54 pm
• Response factor for Refractive index detectors January 21 - 01:02 pm
• HPLC Flow Rate Gradient January 14 - 04:38 pm
• Coupling efficiencies and HPLC analysis of Oligonucleotides January 14 - 05:42 pm
• Is it posible, Solvent Peak response change due to difference in concentration of sample ? January 22 - 04:29 am
• Performance check for Prep.HPLC January 15 - 02:53 am
• How much is a new HPLC ? January 17 - 02:54 am
• Polysorbate 80 Analysis June 12 - 09:01 am
• Validation of Karl Fischer Method for the analysis of Active Drug Substances January 21 - 01:58 pm
• Method for the detection of certain Azocolourants in Dyestuff January 20 - 07:59 pm
• Ballpen color of signatures in GMP/GLP enviromment January 22 - 07:30 am
• Remove proteins from HPLC column January 20 - 11:22 pm
• Improving Solution stability of a platinum complex in water for HPLC analysis January 20 - 04:41 pm
• Any idea how to quantify hydrogen peroxide and/or urea peroxide (carbamide peroxide) January 20 - 07:53 pm
• How to separate NaCl after ion exchange chromatogram? April 9 - 04:01 pm
• How to separate NaCl from desired substance after ion exchange column? January 22 - 05:32 pm
• Ion Exchange Theory with Resin January 22 - 11:04 am
• Different types of membrane filters January 23 - 04:54 am
• Cetylpyridinium chloride January 23 - 05:34 am
• Equivalency of Column Test procedures January 31 - 01:24 pm
• Diastereoisomers in a chiral column January 31 - 03:33 am
• Autosampler contamination February 4 - 12:29 pm
• Sample mass February 17 - 07:50 pm
• HPLC vs CZE vs CEC May 19 - 10:50 pm
• Changing retention times of amino acid analysis with a modified Waters AccQ tag method February 14 - 07:10 am
• What are the possible causes of shoulders peaks and splittin? April 22 - 06:50 am
• A method of itraconazole February 10 - 07:00 am
• Two horrible peaks with the same spektrum - Ru dye komplex hplc February 7 - 01:21 am
• DEGRADATION of ANALYTE by Acetonitrile HPLC grade May 30 - 05:27 pm
• Ion exchange Resin Suppliers February 21 - 10:28 am
• Diode array spectra structure analysis June 27 - 02:08 pm
• Filtered versus unfiltered mobile phase February 7 - 10:42 am
• Has anyone tried USP 26 Isoniazid Injection ? February 5 - 11:29 am
• Difference in tailing factors in the peaks of sample and standard. February 6 - 08:57 am
• Norethinodrel Degradation February 6 - 01:32 am
• Shifting retention time of strongly retained impurities February 6 - 11:40 pm
• Shift in Retention Time in the same run February 11 - 12:36 am
• Extraction of compound from lymph fluid February 16 - 02:44 pm
• CPC analysis February 10 - 12:41 pm
• High Presure With Buffered Solutions February 15 - 12:27 pm
• Problems with a method February 13 - 05:11 pm
• IC jagged peak February 12 - 09:30 am
• UV detector cell inlet tube blocked February 17 - 11:38 am
• Need to make an inject start trigger February 18 - 02:31 am
• 20mM phosphate precipitates in 80% ACN??? February 13 - 05:05 pm
• Dioctyl Sulfosuccinate February 21 - 05:52 pm
• Fluorescence Detector February 18 - 02:32 pm
• RPLC separation question February 15 - 10:34 am
• UV lamps February 17 - 03:59 pm
• Retention times of benzodiazepines February 16 - 02:51 pm
• Structure of HPLC equipment components in power point presentation February 17 - 11:27 pm
• Solvent/Column question March 29 - 04:36 am
• Baseline Drift during gradient elution February 19 - 04:09 pm
• Indirect UV-Detection AND Alkylamides February 20 - 06:01 am
• Unstable Retention Times for Folic Acid? February 20 - 11:29 pm
• PROTEIN DENATURALIZE IN BLOOD SERUM February 20 - 11:39 pm
• 1050 Degasser pump diaphragm February 20 - 06:35 am
• SPE for benzo[a]pyrene February 20 - 03:51 pm
• Kratos Spectroflow 773? February 25 - 11:58 am
• Fenofibrate February 24 - 10:10 pm
• How determine the % ion pair reagent to use February 26 - 11:57 am
• Linearity: Calculations February 26 - 12:01 pm
• Theophylline analysis February 27 - 04:20 am
• Conditioning step in SPE Methods for blood samples. February 27 - 03:12 pm
• Cromolyn sodium HPLC *searching article* February 27 - 11:21 am
• Dissolution Tests Media February 28 - 07:55 am
• Neutralization of gentamicin sulphate June 10 - 05:32 am
• Quantitation of Matrine and Oxymatrine?? June 12 - 07:43 pm
• Purification of an glycoprotein, which is present in proteinfree medium June 26 - 12:35 am
• Dissolution Medium selection for practically water insoluble drugs July 5 - 09:20 am
• I have a retantion time drift problem ...... July 14 - 11:03 am
• How to analyze N,N-diethylammonium N,N-diethyldithiocarbamate using HPLC? July 29 - 04:37 pm
• Ideas for alternative to triethylamine (TEA) in mobile phase July 31 - 05:58 am
• Phosphalipid analysis August 1 - 07:27 am
• HPLC result variation. September 5 - 07:42 am
• VALIDATION OF HPLC March 26 - 04:43 am
• Role of Triethyl amine September 6 - 05:44 am
• Specificity-Fluore detector September 29 - 10:21 am
• Benzoic acid May 26 - 01:16 am
• Organic salts April 28 - 12:35 pm
• Column packings June 7 - 10:37 am
• Biological samples March 31 - 06:45 am
• SPE for Drugs in blood plasma April 24 - 04:20 am
• Separation factor and theoretical plate calculations for gradient mode March 6 - 12:34 pm
• HPLC Analysis of Cu(I) complexes March 4 - 09:48 am
• Pore size in RP Chromatography March 3 - 05:23 pm
• Xterra alternatives March 5 - 06:32 am
• Help in HPLC pump malfunctioning part identification June 19 - 08:23 pm
• Strategies for HPLC method development March 7 - 02:02 am
• HPLC method validation March 4 - 08:54 am
• Column for derivatized amino acid separations March 4 - 11:50 am
• Apparent pH of mixed mobile phase March 6 - 09:19 pm
• Integrated B1, B2 and B6 analysis March 8 - 10:48 am
• Method Validation - Acceptability of Parameters (Ex: Linearity) March 7 - 08:08 am
• Post-column OPA reaction failure with primary amines March 18 - 09:20 am
• Baseline problems with ECD March 17 - 12:07 pm
• Shimadzu software June 12 - 12:58 pm
• Different System behaves differently March 17 - 12:15 pm
• Heterocyclic hydrocarbons analysis March 17 - 09:58 am
• Life of rotor seal March 14 - 03:02 am
• IPC and Gradient Elution March 17 - 09:06 pm
• Accuracy and precision for a biological assay March 14 - 01:31 pm
• Accuracy and precision for a biological assay March 17 - 02:37 am
• Oxalic acid retention May 9 - 02:02 am
• Phosphenates analysis March 17 - 11:53 am
• Pack your own column March 20 - 10:33 am
• Flow Rate effect March 28 - 03:12 pm
• Interactive pump control with Class-VP software March 18 - 10:17 pm
• Problems with Folic analysis March 21 - 03:06 pm
• Shifting baseline after first analyte peak June 15 - 12:29 am
• Prep Chromatoghraphy April 3 - 07:57 am
• Enantiomeric separation of chiral metal coordination polymer March 21 - 02:54 pm
• Venom analysis March 25 - 05:43 am
• Blending solvents with RI detection March 24 - 10:24 am
• Bisamino-PEG conjugates separation ? March 24 - 08:10 am
• How to obtain free bases by prep. LC? March 25 - 03:57 pm
• Method Validation for impurity estimation in formulation March 25 - 05:35 pm
• Pigments March 25 - 02:44 am
• PREPARATIVE CHROMATOGRAPHY March 29 - 09:59 pm
• SPE cartridge... allow to dry? March 31 - 06:20 pm
• How to define raw data March 27 - 07:06 am
• Suitable compound for the determination of the dead time March 27 - 07:16 am
• Problem with retention time consistency June 18 - 08:33 am
• UV-response March 26 - 09:47 pm
• Correlation RT- physical properties April 9 - 06:25 am
• Ion pair problems with TSK gel Super SW 3000 size exclusion columns? March 27 - 12:46 am
• HPLC of quaternary amino group containing compounds April 24 - 09:11 am
• Mobile phase degassing system February 27 - 02:47 pm
• Shodex kc811 March 28 - 06:05 pm
• BHT by SPE - Lot of applications available for BHT. Has anybody investigated... March 28 - 07:26 am
• Plate Theory March 31 - 06:15 pm
• Deivatisation of Sulfonic acid for HPLC/PDA April 10 - 02:12 am
• Carboxylate anoins determination April 3 - 03:56 am
• Analysis of Chromium VI Plating Solutions by Ion Chromatograhy April 7 - 06:08 pm
• Glutathione April 4 - 06:44 am
• Problem with impurities April 3 - 06:03 am
• Does anyone have work with the Zorbax SB-phenyl column? April 16 - 02:11 pm
• Albuterol-assay in drug product April 2 - 12:05 pm
• GRADIENT QUESTION April 8 - 12:35 pm
• Ascorbic acid in cosmetics April 10 - 03:56 am
• Precipitation versus filtration for amino acid analysis of cell culture supernatants April 4 - 06:59 am
• Separation of Triglycerides by LC April 24 - 06:09 pm
• GPC Method for Tinuvin 322 April 6 - 11:56 pm
• Agilent Diode Array - is it good? April 11 - 06:18 pm
• Any methods for ketotifen fumarate? April 5 - 07:11 am
• TLC spot reagents April 14 - 01:56 am
• Set parameter in drylab software April 8 - 10:08 am
• Guard cartridge/column for microbore columns? April 9 - 02:41 pm
• Working with rat plasma April 10 - 11:54 pm
• Dial-A-Mix April 11 - 02:19 pm
• Separation of Proteins : April 17 - 12:40 am
• Instrument vs. Human Mixing May 10 - 08:52 pm
• Any idea