Chromatography Forum

Chromatography Forum: LC Archives

Welcome to LC Archives. You may read any of the discussions by clicking on the appropriate link. To start a new discussion of your own, go to the LC Message Board.

Combinatorial Chemistry May 3 - 01:47 pm

Late Elution April 30 - 02:53 pm

Washing column with 100% aqueous. July 23 - 08:30 am

Chiral Chromatography July 23 - 08:34 am

Blown up detector cell in HP DAD May 24 - 04:07 pm

Need Better Resolution May 20 - 11:20 am

Variable Retention Time in Gradient HPLC of Protein May 18 - 10:06 pm

Analysis of GMP, GDP and GTP May 19 - 09:10 am

Can I detect this by UV? May 20 - 10:13 am

What is an appropriate internal standard for lactate/pyruvate analyses of brain microdialysates? May 22 - 02:01 pm

Analytical Chemistry Laboratory Layout May 25 - 03:57 pm

0,001M H2SO4 as mobille phase May 27 - 12:10 pm

Resin-Based Column July 13 - 10:59 am

Gradient Runs hampered by Water Purity? July 23 - 04:01 am

Quantitation June 10 - 08:25 am

Contamination peaks in TFA and ACN gradient June 16 - 10:05 am

Reporting zero June 11 - 04:51 am

Ion-pairing reagent June 13 - 11:47 pm

HP 1090L HPLC May 31 - 06:18 am

Ultrahigh pressure liquid chromatography June 1 - 12:57 pm

Dextrose Interfering With Lactic Acid HPLC Assay September 22 - 11:05 am

USP columns June 11 - 06:37 am

Method validation June 1 - 07:29 am

PL GEL COLUMS VS PHENOGEL COLUMNS : May 24 - 03:25 pm

Reverse Phase, Large Scale Liquid Column Preparative Separation to Precede HPLC June 9 - 11:23 am

Logbook June 17 - 08:03 am

Direct injection of invitro metabolism samples June 15 - 06:08 am

Quantifying triethylphosphate (TEP) June 8 - 02:47 pm

Method validation June 28 - 08:31 am

UV flow cell cleaning (?) June 11 - 10:38 pm

Anybody make an HPLC column packed with alumina? July 29 - 05:22 am

Second column confirmation June 11 - 03:20 pm

Solvent Filtration June 25 - 03:27 am

DNA with HPLC July 3 - 01:58 am

Cyano Columns for RP-HPLC June 23 - 12:49 pm

HIC problems June 10 - 11:50 am

HAUNTING GHOST PEAKS June 18 - 03:55 pm

Auto-injector plugged with septum cores, Help? June 23 - 09:41 am

Gradient Polymer Elution Chromatography June 13 - 09:11 am

HPLC Equipment Qualification June 25 - 02:46 pm

A smile face gradient baseline June 18 - 03:38 pm

Expiration dating June 23 - 08:50 am

Problems with base noise in the begining of all chromatograms June 23 - 02:34 am

Alternatives to a normal phase separation? June 19 - 02:14 am

Negative Spikes in Sample Peak June 19 - 02:24 am

Self-Service ? October 21 - 07:38 pm

Side bar to Self-Service topic June 29 - 11:26 am

LC Anion Columns June 28 - 11:28 am

Peak shape depends on the wavelength? June 30 - 10:10 am

Glucoseamine by hplc using uv June 26 - 09:44 pm

ACN/Water premixing - any benefits? July 21 - 01:56 pm

Ghost peaks observed in Cation analysis July 1 - 05:34 pm

I need a method/column for detergent analysis May 27 - 06:10 am

Determination of Ascorbic acid-2-phosphate in tissue culture medium May 14 - 11:10 am

Flow Injection Analysis July 8 - 10:13 am

Shimadzu LC-10AT Pump July 7 - 12:55 pm

Cation Exchange Column Needed July 1 - 05:44 pm

Unidentified "Hump" present in ACN/Water gradient July 23 - 04:48 pm

Sample Prep July 2 - 05:41 pm

Need Alternative Column June 18 - 11:49 am

Isomer separation July 24 - 08:45 pm

C8 Columns-How reproducible are they within/between lots? July 1 - 06:20 am

Bovine/human insulin July 5 - 09:22 am

Degasification July 5 - 03:15 am

Guard column July 7 - 04:36 pm

Concentrations for Buffers and Ion-Pairing Reagents July 16 - 04:11 pm

Pyruvate stability July 2 - 01:07 am

Fluorescent Dye Carry-Over June 22 - 01:15 pm

0.4M Ammonium formate July 1 - 10:51 am

Application July 19 - 11:22 am

Organic Acid Analysis July 1 - 04:52 pm

Ghost peaks appear with increased injection volume July 17 - 03:44 pm

Vitamin E Acetate June 27 - 11:56 pm

Loss of uv peaks when using gradient July 27 - 09:59 am

Loss of Response of Hydrocodone bitartrate by HPLC December 4 - 02:57 pm

What should I include in my Chemstation revalidation? July 6 - 02:46 am

Evaporative Light Scattering Detectors July 28 - 04:01 pm

GPC/SEC software July 5 - 02:51 pm

Dyes June 26 - 08:49 am

Amino acid analysis July 7 - 12:56 pm

Microbial degradation of chloroaniline July 19 - 01:31 pm

Air tight solvent bottles July 7 - 01:48 pm

Where can I find syringe ion exchange filters / membranes? July 22 - 10:53 pm

What is the purpose of TFA? July 15 - 09:26 am

PAH's in sediment July 9 - 02:57 pm

Chromatography of chlorophyll July 12 - 03:14 pm

Column Flushing July 16 - 01:26 pm

Bacterial growth in buffered mobile phase July 14 - 03:31 pm

ESA coulometric detectors July 23 - 11:24 am

Troubleshooting LC systems- advice on book purchase July 16 - 09:46 am

Related compunds, maximum level. July 19 - 01:38 pm

How wash the RP-Trimethy column July 20 - 08:04 am

Lichrospher SC125 ? July 20 - 09:25 am

Does anyone know the filling of the Ultrahydrogel 120 column July 20 - 05:17 pm

Baseline problem at the tail of the peak. July 21 - 01:15 pm

Just where is t0 measured ? July 28 - 12:10 pm

Replace existing solvents completely in a HPLC pump April 11 - 01:15 pm

New columns, Hybrid particle technology July 23 - 12:48 pm

HP 1090 diaphram leaks oil July 29 - 06:23 am

Proportioning Problems August 18 - 03:37 am

Need retentive RP-HPLC column that is stable in 100% aqueous August 13 - 12:13 pm

Internal standards and indirect detection in CZE July 15 - 01:38 am

Shimadzu HPLC pump that eats seals and sapphire pistons August 17 - 11:39 am

HPLC Detector calibration for range of use September 17 - 08:22 am

Problematic gradients !! August 16 - 10:16 am

Equipment cleaning validation August 4 - 08:28 am

SFE-Supercritical Fluid Extraction August 3 - 09:33 am

HBsAg Separation August 2 - 06:10 pm

CN Columns using Ion-Pairing Reagents August 18 - 09:42 pm

Last injection is larger or smaller than previous ones August 19 - 11:22 pm

Fingerthight PEEK fittings to HPLC Column August 4 - 10:25 am

Ideas for unusual separation August 13 - 09:19 pm

How can I rid my sample solution of dissolved oxygen? August 6 - 02:03 pm

Method Development for Extraction of Calcium,Magnesium and Potassium. September 27 - 07:47 am

Wavering baseline in standard gradient run August 13 - 06:50 pm

Interference Peaks August 9 - 02:19 pm

Sonication only? August 23 - 02:39 pm

Two-dimensional chromotography August 30 - 10:38 am

Looking for a column August 9 - 01:53 pm

What is the correct way to integrate closely eluting peaks? July 8 - 03:33 am

RP Ion-Pairing Chromatographic Behavior August 7 - 07:50 am

Increasing peak area August 12 - 07:02 am

Extracolumn Effects August 13 - 10:34 pm

Nomura Chemical Supplier/Equivalent August 14 - 01:38 pm

Column Regeneration August 23 - 12:14 pm

Any book of Chromatography Method Development available? Who Knows? August 13 - 08:40 pm

I need a simple method for separating mono- disaccharides and amino acids from herbal extracts August 16 - 05:49 am

Data System Sampling Rate August 17 - 12:00 pm

Definition August 18 - 08:51 am

Separation of kynurenine and kynurenic acid August 23 - 10:58 am

Post-column fluorescence derivatization for anilines August 25 - 01:02 pm

Ghost Peaks in Pure Methanol October 22 - 01:00 pm

Column Volume August 20 - 01:10 pm

Autozeroing the detector August 21 - 02:24 am

Ghost Peak Originating from Buffer August 27 - 01:03 pm

Making a 6N acid solution?? August 29 - 07:11 pm

UPDATE on Abstract "humplike" Peak in blank methanol August 25 - 12:41 pm

HPLC assay of Collagen August 26 - 05:00 am

Hplc method for ambroxol hydrochloride November 10 - 11:28 am

Separation of Furaltadone HCl and Amprolium HCl August 31 - 03:19 am

Citrate analysis August 31 - 08:44 am

Tocotrienol Std's - Merck Isomer Kit #8524 August 31 - 08:52 am

Millenium Software/Peak Quantification October 5 - 04:46 pm

Looking for HPLC method for crystal violet September 23 - 02:18 pm

Baseline Sensitivity Problem due to Room Temperature October 9 - 04:28 am

Chrom Merge October 14 - 12:22 pm

Fraction Collector help September 30 - 02:37 pm

McCalley papers on basic drug performance September 6 - 02:30 am

Standard Solutions September 26 - 10:30 pm

Remote starting Alliance September 2 - 10:34 am

Problems with pumping pure ACN September 29 - 12:59 pm

Hichrom column September 2 - 10:04 am

Tests for columns September 7 - 01:04 pm

What should be the limit of day to day variability of HPLC Assay September 20 - 06:19 am

C8 degradation September 8 - 03:51 pm

Curve Fit Type September 14 - 09:43 am

How many analytes? September 13 - 09:17 pm

Problems with KH2PO4 buffer September 14 - 06:57 am

Methods for detecting caffiene. October 7 - 02:19 pm

Determination of sorbitol in the presence of protein September 8 - 06:43 pm

Changes in analytical method those require validation October 14 - 01:22 pm

Cycling Baseline Noise during monitoring September 15 - 03:47 pm

Detecting tertiary amines? November 14 - 01:50 pm

Microbore chromatography: sensitivity/resolution September 12 - 11:02 pm

Validation/Forced degradation analysis of formulations September 20 - 05:51 pm

Determination of pK? June 19 - 09:26 pm

Quantifying levels of ascorbate and dehydroascorbate in plant tissue September 22 - 04:55 am

Waters Millenium Software (HPLC & GC) May 26 - 03:31 pm

ISOCRATIC HPLC METHOD FOR PCR PRODUCTS September 23 - 10:39 am

Negative deflections September 21 - 07:35 pm

DIFFERENCE IN SENSITIVITY USING 2MM AND 3MM COLUMNS September 22 - 08:31 am

SDS detection query September 23 - 06:47 am

Need opinions on HPLC-ECD systems September 29 - 05:57 pm

Method Development-How to know if a sample is ionized September 24 - 08:55 am

Instrument calibration/validation parameters October 6 - 11:33 am

Indirect conductivity detection September 23 - 02:34 pm

Buffer September 29 - 12:43 pm

CISAPRIDE September 30 - 03:50 am

Germall 115 (Imidazolidinyl Urea) by HPLC January 29 - 04:05 am

Problematic blanks !!!!!! October 15 - 05:23 am

Papers on Radial Flow Chromatography September 1 - 05:51 pm

Labpro Column Selectors with Millenium software October 31 - 01:26 pm

Question on Linearity October 15 - 07:32 am

Buffers and Salt Modifiers October 26 - 08:26 am

Do we need a European Reference Column October 19 - 06:24 am

Looking for a good HPLC troubleshooting book (or course) October 20 - 06:08 am

How would you generate a free anime ? September 26 - 10:44 pm

Quantitation using Evaporative Light-Scattering Detector? October 7 - 06:11 am

Signal to Noise Ratio September 27 - 08:59 pm

Washing columns with water October 6 - 06:45 pm

Calibrating Instrument October 8 - 03:57 am

LLOD/LLOQ September 29 - 06:44 pm

Polymeric columns October 19 - 10:09 am

Cationic Ion Pair Reagent July 25 - 02:52 pm

Determination of MDA in biological samples April 23 - 04:03 pm

Amino Acid Analysis Beckman 6300 October 18 - 08:25 am

Column Choice October 20 - 03:21 am

Sulfadiazine and Trimethoprim in feeds November 3 - 04:22 pm

Needle Wash for Waters 2690 Alliance October 6 - 05:31 am

TRANSITION METAL ANALYSIS USING CONDUCTIVITY DETECTOR October 7 - 01:22 pm

Automation of SPE October 12 - 02:46 pm

APPLICATIONS OF ION CHROMATOGRAPHY IN PHARMACEUTICAL INDUSTRIES October 15 - 07:20 pm

Proanthocyanidins in Grape Seed Extract and Pine Bark August 13 - 04:36 am

Reverse phase method for prednisone/prednisolone May 16 - 06:31 am

Relative fluorescence quantum yield October 11 - 08:27 pm

Baseline-problem with PDA October 24 - 04:05 am

Why Use Chloride and Iodide Salts in TLC Mobile Phase? October 14 - 09:31 am

Plasma sample preparation by protein precipitation October 19 - 11:01 pm

Waters fraction collector problem October 15 - 02:14 pm

Looking for an HPLC method for the assay of ethanol October 22 - 07:17 am

CCC solvent pump October 29 - 02:39 am

Phenylephrine HCl October 19 - 02:38 am

Ammonium Acetate Buffer and Flourescence detection October 20 - 03:18 pm

Vitamin D October 29 - 06:45 pm

Isoeluotropic HPLC solvents October 29 - 12:36 pm

Ion-pair reagent and strength of signal October 27 - 09:39 am

Mm Equivalent for Ion Exchange (SCX) columns. October 21 - 07:23 pm

Millenium 32 - Internal Standard Reporting October 21 - 04:12 am

DIAMIR chromatography software query October 30 - 08:02 pm

% Recovery? October 24 - 07:30 am

Two peeks when changing the matris? October 28 - 12:46 am

Tetrabutylammonium hydrogen sulfate October 26 - 07:30 pm

Bufferchemicals October 27 - 10:07 am

ELSD - Linearization of Signal September 25 - 11:59 pm

Features of importance in Data aquisition software October 11 - 12:52 pm

System Suitability November 15 - 12:21 pm

Bufuralol intermediates September 15 - 03:36 am

Photodiode array detector choice ? December 13 - 06:35 am

HPLC analysis of silver sulphadiazine (SSD) September 17 - 08:10 am

HPLC method for quantification of aminoguanidine in biological samples September 21 - 05:06 am

Tocol Standard - Any suppliers ?? December 21 - 12:14 pm

Formic acid Fouling September 24 - 10:13 am

Benzethonium chloride assay November 18 - 02:55 pm

In need of assistance for CE method for phosphate and glucorinic acid September 28 - 09:07 am

How to reduce Chymotryptic like peptides in Map? September 29 - 12:02 pm

Light Scattering (ELSD) Methanol/THF ghost peaks May 19 - 02:21 am

HPLC data handling software December 22 - 09:04 am

Separation or regeneration ?????? October 14 - 07:50 am

Millenium 32 Default Wavelength November 5 - 11:00 am

Estimating surface coverage December 19 - 07:13 pm

Elution of various analgesics October 21 - 09:05 pm

Analysis of a tertiary amine October 25 - 06:29 am

Diffusion coefficient December 19 - 07:30 pm

Analysis of asprin November 29 - 04:35 am

Ion-Exchange chromatography November 1 - 06:22 pm

Determination af sulphite by ionchromatography. October 29 - 05:03 am

Analysis of pyredine derivat October 27 - 06:51 am

HP chemstation printing problems December 1 - 05:43 am

Urea Determination by HPLC October 28 - 11:15 am

Biocad Sprint RPHPLC October 28 - 01:48 pm

Phenyl-cyano column? May 30 - 11:11 am

Waters 2690 versus HP 1100 December 6 - 02:27 pm

I have a negative peak problem. December 7 - 07:09 am

What is selective absorption? October 31 - 04:19 am

HPLC Method for Green Tea to Determine Polyphenol Content. November 21 - 11:12 am

PDA vs. ELSD - Quantitation issue November 2 - 09:20 am

Looking for any HPLC assay info for Lutein or Zeaxanthin November 29 - 05:57 am

Immobilized metal affinity chromatography December 3 - 01:15 pm

Column Volume November 3 - 08:52 am

ERGOSTEROL November 17 - 06:45 am

TIAMULIN June 5 - 07:42 pm

Phenylpropanolamin+brompheniramine+norpseudoephedrine November 9 - 09:38 am

Question on Cleaning Validation November 17 - 05:04 pm

Ion-pair determination of amino acids November 5 - 12:17 am

Looking for a method for Glyphosate May 20 - 03:46 pm

HPL analysis of oxalic acid and acetates November 17 - 04:05 am

Column selection December 22 - 05:38 pm

AZITHROMYCIN-ANALYSIS-PULSED ELECTROCEM-DETECTOR November 8 - 09:33 pm

Calibration suitability November 10 - 01:11 pm

Propylparaben November 12 - 12:26 am

Best size silica/other product for Chromatography columns November 15 - 07:12 am

PH adjusment of HPLC mobile phases December 22 - 02:22 pm

TLC November 16 - 05:40 am

Information on centrifugal partition chromatography November 16 - 05:28 am

How to analyse sodium acetate using HPLC? November 30 - 12:20 pm

Convert an SDS-PAGE method to CE method November 18 - 04:46 pm

Looking for an HPLC method for the assay of ethanol July 14 - 12:42 am

HPLC/GC problems with tertiary amines October 25 - 03:46 am

Finding the concentration by the area under the graph November 22 - 09:29 am

Priming injection : troubleshooting November 24 - 08:01 am

SGE SS W5-C18 AR column December 8 - 02:22 am

Bacterial growth inhibition by an organic modifier December 15 - 07:19 am

Micromeritics 728 Autosampler Manual December 6 - 06:36 am

Buffer crystalizing December 1 - 08:44 am

Validation of Qualitative analytical methods December 2 - 06:34 pm

HPLC method validation December 14 - 06:15 pm

How to check the method capability? December 7 - 10:31 am

Peaks eluting in void volume region. December 9 - 07:17 am

Removing quaternary amines December 10 - 12:05 am

Degassing mobile phase: He sparging vs. USP December 9 - 02:38 pm

Detector saturation December 16 - 05:18 pm

Paper Chromatography June 17 - 06:08 am

Anyone using older (10+yrs) HPLC systems? March 2 - 02:24 am

Decreasing response factors with increased concentration December 20 - 10:33 am

Vitamin A Analysis. December 22 - 01:42 pm

Transferability of gradient methods December 21 - 03:03 am

Assay versus Method?!!! December 17 - 12:02 pm

Urea Assay December 16 - 10:06 am

Daily validation December 17 - 05:43 pm

Cost-effective source of autosampler vials December 23 - 03:07 am

Detectors December 14 - 04:02 pm

TFA/ACN Degradation December 14 - 08:48 am

Separation of fluoromethyl benzyl alcohol from cholromethyl benzyl alcohol December 14 - 12:50 pm

Shodex kc-811 December 15 - 04:36 am

Stress study for specificity validation May 25 - 10:41 pm

How to Analyse active ingredient in blood/serum December 28 - 04:18 pm

Determination of LOQ-LOD December 31 - 09:21 pm

Detector problems for a beginner December 26 - 01:20 pm

Looking for a column to seperate C , N and S December 3 - 09:26 am

SPE method to quantitate hydrocodone and codeine by gcms December 6 - 06:20 pm

Response factors problems with F-chemicals December 21 - 11:06 pm

Method transfer protocol April 7 - 04:28 pm

HPLC method for cetrimide October 22 - 05:14 am

HELP/ADVICE needed -- Analysis software recommendations December 15 - 08:35 am

Separation Problem: Need Some Suggestions November 27 - 09:00 am

Limit of Detection/Quantitation December 30 - 10:20 pm

Need help locating HPLC method for Cefuroxime Axetil July 1 - 03:03 am

Chiral column January 10 - 08:52 am

Purity factor and validation of impurity January 18 - 12:49 am

Use of ECD in HPLC January 22 - 09:08 am

Determine low levels of Teepol by HPLC June 8 - 04:34 am

Retention time shift July 8 - 09:22 am

Assay method-cetylpyridinium chloride? January 5 - 09:24 pm

PAHs with HPLC May 11 - 01:45 am

LOQ-LOD January 6 - 08:39 pm

Related Substance Method Transfer January 27 - 07:31 am

Calculation of related substances January 5 - 08:10 am

HPLC assay of ketoconazole-help needed January 2 - 10:58 pm

HPLC-column January 31 - 01:19 pm

Integration of the peaks June 29 - 09:34 am

Having big trouble with Vitamin K assay in the presence of Vitamin E May 9 - 02:31 am

Selection of Buffers September 17 - 07:35 am

3rd (strong) mobile phase modifiers in RPLC February 23 - 12:55 am

STOP PRESS STOP FLOW January 14 - 07:50 am

%area vs %w/w January 17 - 12:44 pm

Capturing 2D UV/Vis spectra from Chemstation files January 19 - 06:39 am

HP 1090 Series II HPLC Troubleshooting January 21 - 08:07 am

Symmetry Shield v Luna, HyPurity Elite etc January 21 - 08:14 am

John Dolan's Book January 20 - 11:28 pm

Peak tailing with basic compound April 16 - 02:25 pm

Need info on LC Aspirin Assay in plasma June 30 - 11:48 pm

Normalized Peak Area --??--Lioyd R. Snyder's HPLC Book January 27 - 06:00 am

Mobile Phase Preparation January 27 - 11:15 am

Ion exchange extraction of iodide February 17 - 02:58 pm

Ion pair RP separation of phosphate, silicate, sulfate compounds February 15 - 05:37 pm

How to export data HP-1100 ---> Excel spread sheet. March 6 - 05:46 pm

Spectra Physics February 4 - 06:40 am

LC method for Methylene Blue February 17 - 03:33 pm

Quantitation problems for dextrose in presence of salts February 18 - 01:39 pm

Systempeak March 15 - 05:29 am

Ion Chromatography February 22 - 10:25 am

HPLC conditions for a very polar guanidine derivative February 19 - 08:46 pm

Peak Fronting May 23 - 04:22 pm

A reverse phase column for Plasmid (5kb-9kb) characterization February 11 - 12:01 pm

HP Diode Array Oddity March 7 - 11:16 am

Sorbic acid and benzoic acid in cider February 17 - 07:21 am

Buffer problems?? February 26 - 05:52 am

HPLC UV Detecor vs UV photometer June 28 - 09:24 am

Beer's Law March 11 - 08:09 am

High Load, Low Load January 7 - 08:12 am

Cocamidopropyl betaine February 21 - 07:58 am

Chiral selectors in the RP mobile phases January 13 - 02:17 am

Robustness in HPLC method validation April 13 - 01:01 am

Gradient baseline March 7 - 11:34 am

Ion pair chromatography. February 11 - 03:48 pm

Degradation products for cough/cold formulations September 10 - 08:25 am

ACCURACY OF THE METHOD - for related substances February 11 - 10:15 pm

PEAK PURITY with WATERS PDA detector and Millenium 32 May 25 - 07:39 am

XTERRA COLUMN-USP COLUMN January 25 - 02:10 am

Help! Method info for calcium January 25 - 08:04 am

LOD/LOQ form calibration curve March 17 - 01:27 pm

Recovery problems in determination of riboflavin(vit b2)in dairy February 17 - 05:39 pm

Should you Parafilm the top of your solvent bottle? February 2 - 03:46 pm

Layman questions regarding Dionex, Waters, Agilent February 22 - 10:32 am

Need opinions on ECD and Flourescence Detectors February 24 - 08:54 am

Titanium by ion chromatography February 2 - 12:58 pm

Decrease in HPLC flow yields large increase in peak areas February 1 - 10:35 am

trace analysis of lineal alkyl benzene sulfonates by HPLC (e.g. TEEPOL) February 1 - 11:21 pm

HPLC Colum Flushing February 5 - 01:52 am

Shifting peak February 3 - 03:21 pm

SPME HPLC February 21 - 06:58 am

Dim but nice: Conversion of ppm to %(w/w) February 6 - 06:12 am

Cleaning solvent filters February 11 - 11:05 am

Waters 2700 Sample Manager question February 24 - 09:09 am

Electrochemical detection(IC) for Glucosamine February 28 - 03:55 pm

Surfactants mixtures March 22 - 11:18 am

How to separate Aspartic acid and Ornithine February 17 - 04:59 pm

Separation of lactose from maltose and sucrose February 11 - 10:07 am

Chloraphyll A and Productivity February 11 - 05:17 pm

Method Validation software March 6 - 05:59 pm

HPLC Autosampler RSD problems February 16 - 07:56 pm

Suggestions for manipulating Rt and Peak times February 15 - 07:25 pm

Negative Peaks February 20 - 06:05 pm

Back pressure too high February 16 - 10:53 am

Linearity February 17 - 12:58 am

Related substances May 26 - 04:38 am

Doxycycline in porcine tissues February 18 - 07:24 pm

Ion-pairing RP-HPLC for mutation analysis into DNA fragments May 27 - 08:45 am

Preparative Chromatography February 18 - 12:39 pm

Solvents for loading prep LC samples? March 3 - 03:32 pm

Destroying the binding of drug to protein March 7 - 08:42 am

Plant Chromatography Web Sites February 25 - 12:04 pm

Finding a chromatography solvent February 24 - 05:42 am

LC-Method for tetracyclines and/or penicillines March 4 - 12:57 pm

Separation of Albuterol sulfate and phospholipids March 10 - 03:29 pm

Priming injections February 25 - 12:40 am

Methacrylic acid analysis June 1 - 03:46 am

Mobile Phase Recycling March 10 - 02:27 pm

LC-GC: The do's and do nots. March 8 - 01:50 pm

How to compare various columns? March 12 - 09:54 pm

Do we have to perform additional validation for an equivalent column March 8 - 08:40 am

Clean-Up Method for HPLC ? Need Advice Fast!!!! March 6 - 01:02 am

Column dead time HELP! February 29 - 07:19 pm

HPLC methods for Opiates March 2 - 03:18 am

Column equyilibration with THF March 20 - 08:28 am

Polar compounds March 6 - 01:45 pm

Ionstrenght: Samples and mobilephase March 5 - 09:46 pm

Retention time shift March 14 - 07:26 am

Peak Purity on HP1100 DAD March 8 - 11:17 am

Nitric acid for HPLC cleaning May 18 - 04:27 pm

Retension time getting shorter March 10 - 02:18 pm

Postcolumn conditions to make UV or fluorescent derivatives April 5 - 03:50 pm

HPLC Method for Comparing Instruments March 13 - 07:32 pm

HPLC columns for peptide mapping March 9 - 03:27 pm

Analysis for basic polar analyte in 100% methanol? March 10 - 03:47 pm

Vanillylmandelic Acid (VMA) March 10 - 03:54 pm

Wavy baseline (uv detector) March 16 - 10:07 am

Preventative maintenance/re-calibration of HPLCs. What and how often is needed? March 16 - 09:07 am

HPLC analysis of Deoxyalliin May 10 - 11:40 am

To all method validation experts out there.... May 11 - 06:13 am

Column washing March 20 - 08:31 am

Column Washing with TFA March 24 - 08:02 am

Practical info for validating HPLC method March 23 - 04:02 pm

Method to analyze by HPLC non-chromaphore bicyclic compound March 27 - 04:04 pm

Waters Millenium Software & HP5890 II March 6 - 11:26 am

Dibuthyl phthalate February 16 - 02:45 pm

Cocamidopropyl betaine January 12 - 04:36 pm

Testing of amino acids November 19 - 06:46 am

Phenyl-Hexyl Phase for HPLC May 16 - 02:32 pm

TFA turns yellow? April 17 - 11:02 am

Separation of plasmid DNA on HPLC May 24 - 08:51 am

A reverse phase column for Plasmid (5kb-9kb) characterization April 17 - 01:55 pm

Gradient baseline irregularity April 14 - 09:54 am

LC-GC combo applications March 8 - 02:45 pm

Igor macros for Chromatograms? March 9 - 05:18 pm

SEC/HPLC of low molecular weight (5-7K) Heparin May 10 - 09:13 am

CIP-buffer with sarcosyl for cleaning streamlinecolumn (Amersham Pharmacia) March 12 - 04:37 am

Experience with Nitrogen specific Detector in HPLC March 14 - 01:56 am

GLP question regarding cutting of the weight April 18 - 05:08 am

Bizarre LC problem, comments (very!) welcome April 28 - 08:40 pm

Three peaks and three different %RSDs March 23 - 04:26 pm

Analyse protected pyranose March 27 - 11:40 pm

LC plasma carnitine method March 24 - 08:23 am

Normalized Peak Area March 24 - 09:09 am

Possible matrix effect, but only on a certain LC! HELP! March 29 - 05:17 am

Heme-protein isolation. March 28 - 05:32 am

Lipid hydroperoxide purification March 28 - 05:37 am

HPLC pepsin digest resolution of haem-peptide fragment April 4 - 11:36 am

Problem with EC detection March 31 - 02:44 pm

Methods for Germall (imidazolidinyl urea, or imidurea) March 29 - 07:32 am

Matrix effect?!!!!! Please Clarify March 30 - 07:57 am

Chiral d/l amphetamine separations: LC or GC best?? May 31 - 05:32 pm

Peak Area Variations April 3 - 01:45 pm

Hydrofobic charge induction chromatography April 4 - 06:36 am

Cosmetic Preservatives April 13 - 08:09 am

Ammonium Acetate buffer July 1 - 02:22 am

Quantitative detection of Sodium salt April 6 - 09:32 pm

3 micron columns May 4 - 05:58 am

Frontal Analysis April 17 - 12:11 pm

Van Deemter: H/dp < 2? May 26 - 08:15 am

HPLC analysis for Neomycin, Bacitracin and Polymyxin May 17 - 01:45 pm

S/N problem April 17 - 12:29 pm

BUFFER USED IN LC April 17 - 09:01 pm

Chromatography data systems May 2 - 08:37 am

In-line pH measurement for HPLC applications April 18 - 12:30 pm

Microboard Column April 19 - 03:51 pm

Method validation May 22 - 02:40 pm

UV Detection April 21 - 04:48 pm

Selection of column based on chemistry April 24 - 02:13 pm

Assay versus content uniformity April 24 - 10:03 am

HPLC of sugars (maltose). April 11 - 11:50 am

Quantitative HPLC analysis of impurities May 23 - 07:24 pm

Method for sodium acetate in presence of sorbitol and protein May 25 - 11:52 pm

Pre-run system suitability question April 28 - 04:29 pm

Alternative HPLC method for human insulin? May 19 - 10:20 am

Qualification for a refractive index detector April 28 - 09:03 pm

Linear alkylbenzensulfonat and HPLC May 25 - 11:01 pm

High Backpressure with THF. April 28 - 12:19 pm

LC Separation of very closely related peptides May 10 - 12:14 pm

Priming injections May 1 - 11:54 pm

Ion pair separation of LAS on C18 May 25 - 11:09 pm

Columns for basic compounds May 11 - 05:26 am

HPLC "Calibration" for cGMP May 10 - 08:00 am

Compound for Detector wavelength verification May 23 - 07:00 pm

Check standards May 13 - 12:10 am

Cholesterol HPLC April 12 - 08:59 am

Analysis of Formulation Containing Miglyol 840 May 16 - 12:28 pm

PREDNISONE & PREDNISOLONE DETECTION BY HPLC-UV May 19 - 03:42 pm

Test Mix Reference May 25 - 08:36 am

Coelution of Impurities May 29 - 01:02 pm

Troubleshooting - injection error May 22 - 01:20 am

Unexplained peak using cation-exchange method May 25 - 02:11 pm

Post column derivatisation vs precolumn derivatisation May 26 - 02:36 pm

Resolution Problem May 2 - 11:09 am

Separating large biomolecules (200kDa) May 31 - 02:04 pm

RI Detector injection peak April 28 - 10:35 am

Pigment problems March 1 - 04:37 pm

Ion chromatograaphy March 3 - 07:57 am

Glycerin detection March 13 - 04:23 am

Igor macros for Chromatograms? March 9 - 11:59 pm

Internal Standard May 15 - 08:06 am

Method to quanitate mineral oil May 25 - 05:12 am

Shelf life of THF February 23 - 06:35 am

HP1100 problem March 8 - 11:27 am

The use of IAM chromatography January 9 - 12:55 pm

Export data from PC1000. January 21 - 12:00 am

Allantoin Method!! April 12 - 05:11 am

Benzoic acid October 16 - 05:52 pm

Mistery peak on gradient December 3 - 02:59 pm

Using an Unretained Compound for Determining Column Void Volume November 17 - 04:43 pm

FMOC derivatization problem November 27 - 12:52 am

High HPLC column pressure June 2 - 12:03 am

METHOD VALIDATION December 5 - 10:02 am

Determination of Benzalkonium Chloride in Lecithin Matrix - Please Help! November 1 - 02:27 pm

Transfer of Analytical HPLC Methods December 7 - 06:49 pm

Degassing the mobile phase December 7 - 12:34 pm

RP separation of alkylsulfonates : mechanism ? November 1 - 07:04 am

Sample carryover from sample loop? November 29 - 06:26 am

Turbo HPLC October 4 - 01:27 pm

Waters 2690 and Gradients October 2 - 12:29 pm

Ion Exclusion question March 30 - 10:03 am

HPLC gradient/isocratic run October 3 - 06:33 am

Out-of-specification results / integration parameters October 7 - 03:56 pm

External vs. internal standard calibration October 27 - 08:27 pm

Gradient dwell time June 27 - 09:47 pm

RP-C30 October 25 - 06:35 pm

Sucrose removal October 4 - 05:19 am

Neomycin sulphate December 8 - 12:48 pm

UV and Fluorescence Detector Linearity Range October 9 - 10:27 pm

Pair ion HPLC of nicotine June 29 - 09:05 am

Problems with chromatography October 10 - 07:27 am

LC for methyl salicylate October 10 - 01:10 pm

Cis-trans isomerism by UV detection October 12 - 04:26 am

Low-MW Dextran SEC/GPC October 10 - 05:45 pm

Artifact peaks from 25mM KH2PO4 , pH 3.0 October 10 - 05:49 pm

Peak Tailing October 16 - 10:05 pm

What kinds of molecules have fluorescence? Any theory? October 16 - 02:17 am

Gel filtration October 16 - 01:05 am

Methods of separating water soluble vitamins by HPLC December 7 - 06:31 am

Determination of chloride salt in pharmaceutical substance October 18 - 10:03 pm

Is Preparative HPLC purification of stearyl amine possible ? October 18 - 03:05 pm

Linearity Solution Composition November 8 - 12:45 pm

Preparation of protein samples for amino acid analysis?? November 7 - 10:00 am

HELP ! Bubbles forming during acetonitrile gradient. October 28 - 07:07 pm

Toluene in Non-polar matrix October 24 - 02:37 pm

HPLC Optimization Softwares October 28 - 07:13 pm

Amino Acid Assay After Derivatization April 15 - 07:30 am

Using Perchloric Acid in mobile phase October 27 - 08:02 am

Solutions in the lab October 24 - 12:41 pm

Ion-exchange columns SCX October 25 - 06:39 pm

Pectin citrus October 27 - 08:13 am

Pressure fluctuations October 28 - 06:34 pm

HP 1100 noisy baseline November 16 - 02:28 pm

Large baselinedrift with refractive index detector November 2 - 05:13 am

How to Find an FDA Document October 26 - 05:54 pm

Sample Prep-Centrifuge Speed October 30 - 04:46 pm

Is rpm still a valid unit for centrifugation speed? November 17 - 01:17 am

Trace Analysis of Benzotriazole May 29 - 10:35 am

4 minutes broad peaks November 8 - 05:25 pm

Shareware or Freeware Integration Software November 2 - 03:00 am

HPLC problems June 2 - 12:08 am

How much MeCN is safe on a Shodex KC-811? July 22 - 06:29 am

Glycosphingolipids? December 18 - 11:57 am

HPLC Fittings November 3 - 06:37 am

Methyl methacrylate analysis???? November 7 - 09:57 am

Ipratropium bromide November 17 - 12:52 pm

Statistical tools used for intermediate precison November 9 - 05:04 am

Anomalous (?) Gel Filtration November 24 - 07:48 am

Measurement of piperazine in air November 10 - 03:04 pm

Volatile buffer for acid antibiotic November 16 - 08:39 am

HCl neutralization November 15 - 07:25 pm

K-bar instead of K-prime November 16 - 12:17 am

Heated Transfer Lines for SEC-TREF November 16 - 09:09 am

Increasing HPLC throughput November 20 - 06:53 am

Chloride analysis by ion chromatography June 17 - 05:58 am

Ambient November 23 - 01:44 am

Interfering peak in blank gradient December 3 - 03:24 pm

Primary standard purity calculation November 26 - 03:53 am

Buffers at 3.7 with low Abs. at 240 nm December 6 - 04:19 pm

Separation of macrolides November 27 - 10:03 am

Hexane as ion pairing November 29 - 07:11 pm

Ammelide by HPLC anyone ??????? September 9 - 02:46 pm

Drugs of Abuse December 6 - 10:06 am

Malondialdehyd with hplc December 19 - 12:33 pm

Chromatography Data Systems December 16 - 09:48 am

Suggest me an autosampler December 12 - 02:32 am

HPLC of RESIDRONATE December 8 - 06:10 am

Quantitation of Peaks in the 0.1 - 1% Range December 26 - 07:39 pm

Plant pigments and chromatography December 11 - 08:22 pm

Ergosterol analysis by HPLC December 18 - 08:09 am

Re-equilibrating gradients December 12 - 09:50 am

Re: Huge and sudden drop in fluorescence during gradient HPLC March 10 - 07:26 am

Experation date for solutions December 14 - 01:48 pm

THF in mobile phase and Baseline drift, please help!! December 20 - 07:34 am

Scale-up issues December 18 - 10:45 am

RRTs shifting in gradiet method December 21 - 03:49 pm

Negative peak in isocratic method July 1 - 01:49 am

LC column info December 21 - 11:28 pm

Retention Time Shift December 22 - 01:15 pm

Analysis of samples in n-Octanol by HPLC December 27 - 07:18 am

How to calculate g-1 from rpm October 2 - 04:48 pm

Horwitz formula October 19 - 11:06 pm

DGGE with HPLC? December 4 - 07:18 am

Ghost peak issue January 20 - 09:03 am

Differences between amperometric and coulometric ECD January 19 - 02:01 am

Method Verification Problem January 19 - 02:05 am

Algae formation in water mobile phase/pH electrodes January 4 - 11:59 am

Biotek-Kontron or Kontron HPLC equipment January 19 - 02:10 am

Retention time continuously decreased June 8 - 12:46 am

Determination of glyphosate in water January 12 - 02:42 am

Torsemide, Furosemide, any fluorescence? January 6 - 08:20 pm

GPC in Acetone January 29 - 08:19 am

GFC column calibration January 5 - 04:38 pm

ELSD info? June 2 - 07:28 am

Separation of secondary and tertianary hydrophobic amines February 28 - 12:54 pm

Vancomycine - HPLC January 17 - 01:25 am

End capped columns February 6 - 01:01 pm

Why does flow rate affect peak area? January 5 - 03:49 am

Which HPLC system is best for protein analysis? May 23 - 09:06 pm

Retention time variation January 2 - 08:37 am

Polymeric content January 5 - 03:04 pm

Problems with analyte carry-over January 10 - 05:05 am

Analysis of basic, hydrophobic peptides January 30 - 05:35 am

LOQ minimum concentration January 9 - 11:37 am

Blocked frit February 22 - 08:55 am

Compacted gel January 10 - 12:10 pm

Response Factor February 6 - 04:30 am

Phase collapse February 19 - 05:21 pm

Fronting peak and poor recovery at low concentrations January 25 - 11:47 pm

Zero Intercept for Cal Plots January 18 - 12:33 pm

RP-HPLC Column Equilibration June 5 - 09:50 am

Lofepramine from serum July 18 - 07:33 am

Fluorouracil and Buffer vs. Water for Mobile Phase January 24 - 12:21 am

Alpha Lipoic acid and Pregnenolone January 23 - 08:03 am

Area Under Curve Units January 24 - 06:32 pm

Old double destilation with MnO4K, could anybodie explain that method? January 24 - 01:33 pm

Retention time shift January 25 - 03:56 pm

Alcohols and pH August 2 - 06:48 pm

SymmetryShield for sulfa drugs January 31 - 06:00 am

Narrow-bore HPLC columns - resolution January 29 - 02:48 pm

Narrow-bore HPLC columns - resolution February 28 - 05:46 pm

Normal phase chromatography June 4 - 02:41 pm

Negative peaks January 25 - 11:35 pm

Porphyrin Analysis February 27 - 01:53 am

Wavelenght justifiactions February 1 - 11:07 am

Refractive index and electric current January 29 - 10:00 am

Precolumn labelling January 30 - 08:33 am

Chromatography software February 5 - 06:13 pm

Chromatographie of organic pigments and dyes June 17 - 05:34 am

HPTLC February 16 - 02:24 am

Any HPLC methods for separation and identification of Maillard reaction compounds ? March 21 - 09:59 am

Validation / Accuracy question March 16 - 01:37 pm

TLC SPRAY REAGENT March 30 - 11:41 am

Clinical applications of HPLC July 18 - 08:11 am

GPC-Molecular Weight Distribuiton Plot February 1 - 11:18 am

HPLC method development software February 6 - 11:15 am

Tailing peak in ion pair RP chromatography February 6 - 01:18 am

TLC Spray Reagent January 25 - 08:46 am

GPC-Molecular Weight Distribuiton Plot February 6 - 07:04 pm

Problems with test for related substances of Apramycin February 5 - 01:01 am

Doxycycline standars February 8 - 07:58 am

Trimethoprim by HPLC February 18 - 09:56 am

Agilent/HP1050 LC series service and repair February 13 - 08:49 am

Impurities in methanol February 7 - 10:56 am

Method for Sodium Acetate February 12 - 03:35 pm

Sulfinic and sulfonic acids separation May 28 - 10:54 am

Ghost peak source? February 8 - 03:15 am

Acetic acid, trifluoro- vs trichloro- February 9 - 07:38 am

Sample preparation for hplc February 9 - 11:09 am

HPLC method for a compound with pKa 2.3 and 9 February 15 - 09:53 am

Determination of sulfonamides in meat February 8 - 05:19 pm

Maleic acid/ Fumaric acid February 12 - 09:52 am

Hydrophilic Interaction Chromatography February 15 - 02:03 pm

Linear regression vs response factor May 15 - 08:12 am

Acidic and base separation August 8 - 03:34 am

Angelica lactone October 11 - 08:13 am

Chromatography about the lipids October 4 - 07:51 am

Determining the component percentages of solvent blends. October 11 - 08:11 am

Peak tailing October 16 - 09:40 am

Software that reads ASCII data from a serial port - of use to you? October 19 - 07:37 am

Phospholipid analysis? April 13 - 08:06 am

Removal of Ion Pairing Agent from Collected Fractions. April 18 - 10:02 am

Determination of Matrine(Alkaloid) by HPLC June 8 - 06:05 pm

Pancuronium Bromide Peak Shape March 2 - 09:36 am

Identification by HPLC Retention Time March 30 - 09:33 pm

Peak purity interpretation using PDA May 4 - 10:03 am

ELSD info? March 13 - 02:07 pm

Hydrophobic collapse April 17 - 10:44 pm

Variable volume injection for quantitative analysis May 25 - 10:04 am

Column May 8 - 08:56 am

Erythromycin HPLC Assay June 22 - 05:32 am

Affect of pH? April 16 - 12:56 pm

Flash Chromatography January 21 - 12:56 am

Solvent elution strength May 3 - 01:26 pm

METHOD VALIDATION April 27 - 02:45 am

HPLC method for L-carnitine March 12 - 04:08 pm

Is RI with a Reverse Phase gradient possible? March 12 - 04:26 pm

Problems Getting Reproducible Areas in Samples April 6 - 07:21 am

Ion Chromatography August 20 - 07:53 am

Determination of protein in Polysaccharide (Alginate) May 14 - 12:14 am

Ion-pairing column clean-up April 3 - 10:33 am

HPLC of Bromo Compounds February 10 - 03:26 am

Agilent/HP 1100 and 21CFR11 April 25 - 12:16 pm

Hydrophobic Interaction Chromatography (HIC) February 28 - 05:24 pm

LC Method for detecting MTBE in tap water. February 13 - 08:36 am

Anion Exchange Column February 20 - 04:53 pm

Tailing Aldehyde Peak February 14 - 03:10 pm

Comparison of two sets of data February 15 - 10:02 am

Assay Method for Nuosept C (a preservative) February 14 - 07:08 am

Guard column stability February 17 - 04:11 am

6-beta-OH-cortisole February 16 - 06:23 am

Fluconazole, any fluorescence? February 22 - 11:16 am

UV-Vis absorbance detector vs. photodiode array detector February 20 - 04:50 am

Which chiral columns to choose ? March 19 - 06:06 pm

PL Gel or Zorbaz for GPC? March 3 - 12:58 pm

Agilent vs MacMod Zorbax Columns March 7 - 02:36 pm

Ion Pairing: Retention Continuously Increased? February 27 - 06:52 am

HPLC in plants studies; increasing column life February 28 - 04:30 pm

Baseline Correction in Gradient HPLC March 7 - 04:58 am

Gradient baseline/ghost peaks/strongly retained impurites March 3 - 10:06 am

LC of gluconic acid April 10 - 03:45 pm

Ion Pairing- Reference Article/Text Recommendation? July 18 - 01:13 pm

Negative deflections and electrochemical detection June 29 - 04:52 pm

Clean up methods for small volumes March 2 - 03:32 pm

Masking agents in RP gradient HPLC April 4 - 06:08 pm

Retention time variability March 12 - 10:55 am

Drug stability and sample solution stability March 8 - 10:12 am

LC column acidity March 7 - 04:27 pm

Periodically occuring peaks when monitoring baseline March 21 - 06:04 am

Flumazenil HPLC Assay March 11 - 12:47 pm

Accuracy at LOQ for impurity methods including clenaing methods March 12 - 08:39 am

How to choose ISTDs for bioanalytical HPLC methods March 17 - 06:02 pm

Should Flow Rate Effect Detector Response Like This? April 4 - 06:25 pm

How does beffer concentration affect resolution? March 14 - 04:17 pm

Separation of folding intermediates March 14 - 04:56 pm

Buffer Preparation March 14 - 08:59 pm

How do co-eluting compounds affect UV signal? March 17 - 06:15 pm

Negative peaks with Diode array March 21 - 07:34 am

Selenonurea by HPLC ? March 15 - 08:25 am

CLEANIN VALIDATION FORMULAS ?????? April 22 - 09:30 am

Paper chromatography March 17 - 03:35 pm

HPLC pump validation tests May 15 - 04:51 pm

Digital Liquid flow meter for HPLC March 21 - 08:49 am

HPLC for DEOXYPYRIDINOLINE March 22 - 06:28 am

Ruggedness March 27 - 07:18 pm

Gradual flow increase March 29 - 03:24 pm

Turbochrome Navigator software problem March 31 - 09:43 pm

System Suitablity for Protein QC Methods March 23 - 09:31 am

Buying a new HPLC system; what should I do? March 31 - 09:32 pm

Pressure Change During Isocratic Run March 28 - 05:40 am

PH of sample--split peaks April 12 - 05:24 am

Asimmetry factor March 29 - 10:38 pm

UV Cutoff Below 190? March 29 - 08:59 pm

Dimethyloctylamine pH range? April 11 - 05:31 pm

LOD for TLC Dragendorff's Reagent March 15 - 06:01 am

Ion pairing and optimum pH April 9 - 05:06 pm

Solvent recycling........ April 3 - 04:02 pm

Modified starch analysis by GPC!!! May 1 - 12:02 pm

Mobile phase April 11 - 12:34 pm

Mass balance calculations April 3 - 12:54 am

Turbulent flow chromatography. June 21 - 04:01 pm

Method Transfer (HPLC/GC) April 14 - 05:17 pm

Baseline drift April 6 - 12:42 am

HPLC OQ April 5 - 03:31 pm

Assay of silicone dioxide April 13 - 11:59 am

Iso-nicotinic acid hydrazide April 8 - 09:42 am

Intermittent Instrument Failure April 5 - 03:14 pm

The LiPF6 and solvent (like ethylene carbonate) analysis by HPLC!!!!! April 5 - 05:08 am

Comparisons of Drylab software and MODDE, what are the differences?? February 11 - 03:10 am

Chiral analysis April 5 - 04:04 pm

chiral hplc analysis April 6 - 01:46 pm

Polar embedded group April 6 - 10:15 am

Is hplc of acarbose antidiabets by uv detection is possible and how July 7 - 09:30 am

How to measure resolution? April 6 - 09:35 am

Cyanocobalamin (B12) May 24 - 08:40 am

Carry over in protein HPLC analysis April 9 - 06:55 am

System Backpressure April 9 - 10:27 am

Extracolumn effects April 11 - 07:12 pm

Calbration Curve April 8 - 07:41 am

HPLC Detectors May 24 - 09:31 pm

Setting up a stability protocal August 11 - 10:57 am

Increasing retention times April 11 - 01:04 pm

Analysis of Phosphocholines April 11 - 08:50 am

Ion chromatography April 10 - 08:21 pm

Ion pair chromatography April 11 - 03:16 pm

Seeking advice on data acquisition systems - ChemStation or TurboChrom? April 25 - 08:03 am

IS IT POSSIBLE TO DO ANALYSIS WITH A NEGATIVE April 14 - 05:32 pm

Broadened peak/poor resolution April 12 - 10:38 am

Decreasing retention times April 18 - 07:59 am

21 CFR Part 11 April 12 - 01:31 pm

Fluconazole HPLC Methods April 13 - 05:08 am

Salmeterol April 13 - 09:49 am

Cyclodextrines - univeral method April 16 - 10:25 am

Where to find pHa April 19 - 03:33 pm

HPLC of organic acids, aldehydes and ketones April 18 - 10:06 am

Bulk capture purification May 3 - 01:38 pm

Impurity detection and s/n ratio. April 25 - 02:01 pm

Base line derive May 2 - 02:54 pm

Lithochols�ure April 22 - 10:32 am

Decreasing standard peak's area count? April 20 - 10:53 am

Positive baseline drift April 20 - 07:56 am

Hey I.D. you forgot to write what is Se under "CLEANING VALIDATION FORMULAS" DATED March 26 May 11 - 06:47 pm

Peak before dead time... April 25 - 07:54 pm

Peak Purity April 23 - 10:45 am

Post Column Reactors April 30 - 02:57 pm

Post Column Reactors April 24 - 06:27 am

Signal to noise ratio May 24 - 08:10 am

Wavelength accuracy test on fluorescence detectors April 24 - 06:54 pm

Preparative Chiral Separations. May 1 - 08:37 am

Call for molecules knowledge February 29 - 02:38 am

21 CFR part 11 -- need to save interim integration results? May 12 - 07:34 pm

Pka value May 3 - 06:59 am

Noise level April 26 - 07:46 am

Max. Column Size for Normal HPLC System April 27 - 11:02 am

Measurement of individual peak areas of overlapped peaks April 27 - 12:17 am

Calculate %RSD in Excel May 9 - 09:29 am

Comparison of hydrophobicity of c8, c18 and PS-DVS May 10 - 01:10 am

Beta-lactam standard May 7 - 07:54 am

Tropin Alcaloids May 7 - 01:32 pm

LC surfactant polymer. Please HEALP!! May 10 - 11:04 am

Sennosides standards (C-G) May 18 - 08:34 am

Separation of GST-fusion protein from its partial degraded product May 11 - 12:03 am

Mobile phase recipe May 10 - 08:11 am

Method Development Questions (mobile phase/sample prep) April 21 - 03:52 am

Baseline drop in ACN:H2O:TFA system May 18 - 07:25 am

Identification of degradation products for IND and NDA? May 16 - 06:17 am

Sample Injection volume? May 11 - 12:47 pm

Internal Standard for PAH analysis May 21 - 10:39 pm

Need name/supplier for HPLC accessory please! May 14 - 11:39 pm

IQ, OQ, PQ and Calibration May 11 - 07:41 pm

Gilson 321/322 HPLC pumps May 31 - 05:47 am

Buffer for size exclusion May 15 - 03:31 pm

Mystery Peak June 7 - 01:59 pm

Taurine Assay May 16 - 05:58 am

Separation of peptides from simple sugars and organic acids May 17 - 03:16 pm

HPLC H2O May 22 - 01:41 pm

Hitachi HPLC Series 7000 Purchase? Good or Bad Idea? May 11 - 01:36 pm

"aqua columns" May 25 - 06:54 pm

2-naphthylamine analysis May 18 - 09:00 am

Should I buy an Agilent 1100? May 31 - 02:41 am

COLUMN USED IN REVERSE??? May 22 - 03:38 pm

Avoparcin by HPLC May 23 - 05:47 am

Acetonitrile HPLC waste May 10 - 11:30 am

Limit of detection and quantification June 17 - 07:08 pm

HPLC Aftermarket Mixer May 22 - 11:11 am

Between Waters and Varian May 29 - 08:42 am

Avoparcin by ion-pair chromatography May 23 - 02:41 pm

Analysis of Cefdinir for related substances in the bulk drug. May 23 - 05:55 am

Validation of dissolution method May 31 - 01:40 am

System Suitability May 13 - 11:16 pm

Amoxicillin in feedstuffs May 24 - 12:59 pm

Methodology questions June 1 - 10:50 am

Replicate analysis and method validations May 25 - 03:45 am

HPLC May 29 - 05:37 am

Laboratory Power Protection for Analytical Instrumentation May 29 - 11:56 pm

SP8780 AutoSampler June 21 - 08:34 pm

Single RP HPLC method to quantify metformin and glyburide in pharmaceutical preparations June 22 - 05:42 am

How to mesure Vd in LC? June 8 - 05:09 am

Q: Nova-Pak C18 column. June 4 - 03:04 pm

Column Wash! When can it "kill" a column? June 1 - 08:29 am

Acetonitrile polymerization on HPLC system? June 19 - 11:43 pm

Anormal high value for only one component out of three during method transfert May 31 - 08:09 am

Can I improve bipyridine peak shape? May 31 - 02:56 pm

Q: HPLC and LABNET June 5 - 07:29 pm

WHAT IS THE REASON BEHIND THE PEAK FORNTING AND PEAK TAILING AND HOW IT CAN BE SOLVED June 1 - 12:31 pm

Unwanted protein co-purification by affinity chromatography June 1 - 02:38 pm

N-nitrosodiethanolamine June 1 - 12:46 pm

Tannins of Terminalia chebula June 4 - 03:58 pm

Qualification June 6 - 02:24 am

PKa June 5 - 02:16 pm

Decreasing Retention Time, Increasing Standard Peak Areas June 8 - 07:44 am

Problems with Sodium Sulfadimethoxine New HPLC Method in USP June 6 - 07:33 am

Cyanogenic Glycoside Purification June 6 - 09:37 pm

HPLC relationship between flow and retentiontime June 7 - 11:38 am

Gradient elution June 13 - 08:37 pm

Baseline positive drifting of ELSD without column June 14 - 12:06 am

How to seperate methacrylicacid fom acrylacid with hplc June 14 - 04:54 am

HFE-300 or Ethoxynonafluorobutan June 8 - 05:45 am

Fast Method Development June 11 - 12:04 pm

Resolution between two closely absorbing species June 18 - 06:20 pm

SPE cartriges for desalting peptides June 18 - 11:34 am

Ion pair chromatography June 11 - 03:28 pm

Peak shape problem because of contaminations ? June 14 - 01:00 am

Monolithic Column June 19 - 05:55 am

Separation of Potasium Sorbate and Sodium Benzoate in a mixture June 15 - 06:43 pm

Agilent 1100 Problems June 20 - 07:11 am

Sibutramine analysis by hplc June 22 - 05:28 am

Variable Rfs for phospholipids in TLC June 19 - 11:56 pm

Looking for Nomura Chemical Co. June 18 - 05:15 pm

Method Transfer Problem June 21 - 02:24 pm

Millennium and LIMS interface? June 19 - 10:54 am

Q: Pressure change during column cleaning. June 24 - 07:38 am

Column plugged with salts? June 20 - 12:18 am

HP1100 frequent frit change June 22 - 08:35 am

HPLC of small keto acids June 27 - 07:40 pm

HPLC VS HPTLC June 21 - 04:43 am

Problem with DAD detector June 24 - 11:43 pm

Agilent 1100 software question, 3D FL scans June 28 - 05:39 am

Protein binding in biological samples June 26 - 12:16 am

Hydrophobic interaction June 28 - 05:58 am

Decreasing retention times June 28 - 09:04 am

21 CFR Part 11 June 28 - 02:00 pm

Peak purity with HP1100 DAD June 29 - 04:57 pm

Chromatography Software July 26 - 12:15 am

Help/Information on GC May 26 - 05:13 pm

Supplier for deuterated Hexanal and/or deuterated standards in general ?? June 14 - 08:37 pm

Packed Columns and the USP June 13 - 12:45 pm

Estrogen analysis June 18 - 12:01 pm

Alkyl phosphate metabolites June 19 - 07:45 am

Acid oleic June 25 - 03:27 am

Is it possible to analyis thioctic acid and l-ornithine l-aspertate by using single chromatographic system June 11 - 05:53 am

Glycolic acid July 16 - 09:07 am

Cleaning ESA guardcell 5020 June 21 - 10:54 am

Mehod of analyis for thioctic acid and l-ornithine l-aspertate(lola)in combination June 22 - 05:38 am

Dimethylglioxime Analysis June 27 - 03:54 am

GPC analysis using Millennium 32 June 29 - 09:25 pm

HPLC of Cinitapride February 20 - 09:06 am

How to evaporate highly aqueous eluate? March 11 - 03:42 pm

Ion Exchange Chromatography April 7 - 12:06 am

Liquid Chromatography April 2 - 10:06 pm

Single broad "peaks" during ECD of catechol amines April 10 - 04:17 pm

Is it possible to analyse chondroitin sulphate and glucosamine in combination for the formulation April 13 - 10:28 pm

THF to wash column daily ? May 10 - 05:16 pm

Ion pair chromatography May 26 - 07:23 pm

Effect of Particle diameter on phase ratio February 5 - 01:46 am

About flow rate and column i.d. February 7 - 05:13 am

What is the effect of presssure drop on peaks? June 18 - 07:51 am

What is the logic behid the RSD FOR THE ASSAY DETERMINATION.and sequential injection of std and samples. July 16 - 12:24 pm

PE 900 series interface box? April 13 - 02:49 pm

Asking about the packing material of Bilepack II June 2 - 12:47 am

Autosampler from CTC-Analytics June 26 - 12:30 pm

CombiPAL autosampler January 16 - 06:51 am

How long the run time? December 30 - 10:33 pm

Tissue Hydrolysis - AA analysis July 17 - 06:17 am

Monolithic Column July 10 - 01:18 pm

Starting analysis of peptides and proteins July 8 - 11:41 am

Frits versus sieves (screen) July 12 - 08:01 pm

pKa values July 19 - 03:46 pm

HELP!! High column back pressure July 2 - 12:07 am

Merck Chromolith Columns July 5 - 10:50 am

Is it possible to analyse nateglinide and metformin in combination July 1 - 01:26 pm

Flow halved, peak doubled ? July 4 - 02:37 am

List of electrochemical compounds with voltage applied info? July 7 - 06:31 am

Methionine oxidation July 7 - 03:08 pm

Decontamination of a HPLC System from pencillin spores July 3 - 09:43 am

Agilent 1100 Series VW Detector (Model G1314A) July 3 - 02:47 pm

Durability of organic HPLC eluents July 6 - 09:01 am

Method Validation at the QC Site??? December 30 - 10:44 pm

Distorted peaks July 16 - 09:31 am

Calibration curve reproducibility problem July 12 - 07:46 am

Pressure drop July 14 - 05:45 am

Neo-Heliopan Hydro in sunscreen products July 10 - 04:03 pm

Waters 490 Uv-vis any good? July 12 - 11:16 am

Using bovine serum albimin July 12 - 11:22 pm

Electrochemical detection problem July 24 - 10:29 am

Mystery Peak July 13 - 01:27 pm

Negative Peak Problem July 19 - 07:30 pm

Gentamicin sulphate July 31 - 03:09 pm

Semie preparative LC July 16 - 07:00 am

Holmium filters source July 18 - 06:33 am

Preparative HPLC books July 16 - 07:15 am

Cation Choice in PO4 Buffer Prep July 30 - 06:24 am

Monitoring wavelengths/interferences July 17 - 07:41 am

Agilent 1100 'Gameboy' February 12 - 01:36 am

Non-zero y-intercept for Linearity of HPLC Assay July 23 - 05:12 am

Windows driver for HP-IB 82335 IEEE-488 interface card. July 17 - 12:13 pm

Problem with using Waters Millennium 32 Chromatography Manager. July 19 - 04:38 pm

Baseline Noise July 18 - 03:33 pm

Liposome analysis for phospholipid content using HPLC/ELSD? July 19 - 11:26 pm

Peak area differences with different columns, but identical method and system? July 24 - 10:30 am

Do I need a analog to digital converter for collecting data by serial port? July 20 - 12:11 pm

Rejection of level in calibration range for plasma analysis July 20 - 03:23 am

Isocratic Hold June 23 - 05:41 am

Help for practical work of capillary electrophoresis November 22 - 03:11 am

Ammonium bicarbonate for high pH gradient application July 25 - 08:23 pm

I made a mistake in my method and ran air through my column all weekend at 40C; is there anyway that i can save the column?? July 24 - 11:14 am

What do you guys think of Shimadzu LC's?? August 21 - 11:51 pm

Borate buffer July 31 - 03:33 pm

Tailing Factor Optimization for Sulfonates July 30 - 12:38 pm

Pharmaceutical QC Question July 6 - 07:28 pm

Chloro/Fluorosilicons November 29 - 01:31 pm

Separation of angiotensins December 4 - 01:06 am

Separation of closely related peptides (angiotensins) and the use of TEAF December 4 - 08:29 pm

Counter current chromatography July 17 - 10:50 pm

Uronic acids and monosaccharides August 22 - 01:11 pm

Cation Exchange Column August 26 - 07:14 pm

Amoperometric Detection of Carbohydrates August 22 - 01:59 pm

Sulfobutylether-beta-cyclodextrin August 22 - 02:21 pm

N-hyroxymorpholine July 2 - 01:15 pm

Project Assurance Function under Prince 2 methodology of Project management July 3 - 01:11 am

Signal drift in analysis of glycols August 22 - 04:15 pm

Solid phase extraction July 13 - 08:39 am

Isolation of natural compounds by HPLC July 13 - 12:39 pm

Ammonium Hydroxide buffer? August 4 - 11:08 am

HPLC Accuracy and Precision August 6 - 09:14 am

Dioctyl sulfosuccinate sodium by HPLC? August 2 - 09:59 am

Stability of Torsemide and Furosemide July 31 - 05:16 am

Small Scale HPLC - - Why? How? August 9 - 03:39 am

Air pumped on column August 8 - 11:18 pm

Ketotifen - methods available? August 4 - 07:12 am

Strange baseline August 31 - 12:32 am

Octyl Methoxycinnamate by HPLC? July 5 - 05:23 pm

Cleaning polystyrene-divinylbenzene GPC columns August 29 - 12:59 am

Triamterene and HCTZ August 16 - 06:25 am

Reverse flow increases resolution May 6 - 09:45 am

Buffer preparation August 19 - 11:43 pm

Factorial design in HPLC analysis August 20 - 09:55 pm

Chemical database with informations on how to synthetize them August 21 - 07:33 am

Impurity percent changes using diffrent HPLCs! August 29 - 01:00 am

Where to look for existing methods? August 29 - 12:33 am

Improper AIA conversion August 28 - 08:52 pm

DNA purication!!! August 28 - 09:05 pm

Purity analyses August 31 - 10:18 am

How to set up limits for known impurities not listed in USP December 25 - 02:31 am

PH of mobile phase & its effect on retention November 20 - 09:58 pm

Beta-lactams by HPLC November 16 - 07:17 am

Counter current chromatography November 16 - 08:06 am

Change in chromatography by switching to a premix mobile phase on a gradiant December 5 - 08:18 am

Regulatory Requirement for LC method November 20 - 10:09 pm

Isomeric Separation Using LC September 3 - 01:04 pm

Isoflavones in soy preparations October 31 - 07:01 am

Normal-phase HPLC Handbook September 7 - 09:14 am

Preparative scale chromatographie November 19 - 09:28 am

Oleic Acid Assay??? September 5 - 02:30 pm

Unknown Peak September 10 - 08:31 am

Carbohydrate analysis September 8 - 06:57 am

How to increase sensitivity? September 10 - 02:57 am

Non-polar lipids with HPLC-ELSD September 5 - 02:00 pm

Elution strength of sulfurous mobile phases September 4 - 07:43 am

Ubidecarenone (Coenzyme Q10) September 6 - 04:45 am

Bio- Rad 1350 and ValueChrom instrument interface problems September 6 - 08:04 am

Gel Manufacturers for CGE September 6 - 08:42 am

FPLC Valve September 6 - 06:40 pm

Linearity & Tailing Factor September 7 - 08:23 pm

Recovery does not correspond to Linearity data September 11 - 06:41 am

Method Development & Method Validation November 28 - 06:07 am

What is the Typical Concentration for Impurity Profile Analysis September 10 - 09:55 am

Chromatographic purity / Related substances methods December 14 - 12:37 am

Mobile Phase preparation: "Dial a Mix" October 23 - 04:06 am

Quaternary ammonium chlorides December 6 - 07:47 am

Phenethylamine by HPLC September 11 - 01:37 pm

Spherical or irregular silica for LC column October 22 - 04:36 pm

Carbohydrate analysis October 25 - 08:43 am

ECD METHOD DEVELOPMENT October 25 - 08:36 am

Clopidogeral hplc method October 25 - 12:07 am

Probleam regarding amino column October 26 - 02:54 pm

Cleaning LC syringes/needles to decrease contamination December 26 - 10:54 am

Ion pairing reagent levels October 30 - 05:33 pm

Radioactive contaimination in HPLC from use of FSA October 29 - 06:32 pm

Decreased sensitivity November 6 - 11:59 pm

6-methyl-thiouracil October 30 - 06:43 am

MULTI-DIMENSIONAL CHROMATOGRAPHY November 2 - 05:45 am

Polysorbate 80 -post column derivitization November 1 - 08:01 am

Active adhering to LC column November 1 - 04:14 pm

Contamination in autosampler. November 2 - 05:05 am

From TFA to Formic acid November 2 - 04:51 pm

Precipitation of plasma proteins. Organic vs acid? November 5 - 02:47 pm

Reporting results for impurities November 7 - 06:47 am

Baseline drift during a gradient run November 7 - 11:33 pm

Nano-LC November 28 - 08:05 am

Separation of ethyl lactate(enantiomers) November 8 - 07:45 pm

Mysterious peak in D-Lactate standards, separation of D and L Lactate? November 12 - 09:08 am

Sucrose assay November 15 - 06:41 am

Analyse of plant xanthophylls November 16 - 06:01 am

Thiomersal HPLC analysis November 9 - 02:25 pm

Selectivity and Specificity/Dynamic Range and Linearity Range November 10 - 12:00 pm

PDA Detectors November 13 - 12:41 am

Hplc-column information? November 12 - 12:08 pm

Column probleam November 18 - 01:09 pm

Chromatography data systems December 1 - 06:51 am

DMF in Rp mobile phases November 18 - 01:12 pm

Column Storage November 21 - 12:46 pm

Mobile phase with basic pH September 1 - 06:35 pm

Hitatchi amino acid analyser November 15 - 08:56 am

Amine Modifiers and Cyano Columns November 18 - 01:22 pm

RP-HPLC Columns with Embedded Polar Functional Groups November 20 - 08:56 am

Immunoaffinity elution problems November 16 - 03:06 am

Sample Cleanup by solid phase extraction November 20 - 04:49 pm

Baseline of MRM Detection November 16 - 12:48 pm

LD / LQ of impurities November 17 - 09:51 pm

HPLC method for abietic acid and esters April 2 - 03:19 am

Analyzing Sulfite in Proteins December 6 - 08:03 am

Effects of PEG300 in 5% dextrose on the chromatography December 3 - 12:26 pm

Large scale separation of small peptides by HPLC November 21 - 08:50 am

Urine Sample Collection for Drug Analysis December 17 - 08:26 am

Negative peak in Normal Phase HPLC November 28 - 12:08 am

HPLC Analysis of Dimethyl Sulfoxide November 28 - 10:52 am

Regulatory forums for HPLC November 28 - 02:44 pm

Hypercarb coloumn and Nonyl/Octylphenole plus NPEO�s December 19 - 11:28 pm

How to determine the purity percent of pesticide active ingridients November 29 - 06:14 pm

Sample prep December 1 - 05:05 pm

Chito oligosaccharides December 5 - 07:12 am

Drug Peak in Blanks June 9 - 05:55 am

Uranium estimation by Ion Chromatography December 13 - 12:07 pm

Calculation of relative response factors of Impurities in HPLC December 6 - 02:17 am

Chiral AGP column? December 8 - 10:18 am

Amine Modifier Concentration December 6 - 10:10 am

Need HPLC December 6 - 10:42 am

Method Development using ChromSword December 19 - 04:09 pm

Difficult Elution from SP-207 Resin December 8 - 04:41 am

Increased Capacity with methanol December 8 - 05:11 am

COMPUTER RELATED SYSTEM VALIDATION MATERIAL December 10 - 10:18 am

RT's not constant when using a gradient December 14 - 07:29 am

Cysteine & Methionine Seperation December 26 - 04:31 pm

Ion pair chromatography December 18 - 06:58 am

Weight of Calibration Curves December 26 - 05:37 am

LC assay for alphaKG and/or succinate December 21 - 02:12 am

Where can I find specifications for acceptable amount of Penicillin contamination? December 19 - 09:54 am

Differences in resolution December 31 - 07:27 am

Negative Peaks for RP-HPLC December 27 - 03:56 am

Who can help me?a problem about the filter plate for hplc December 21 - 10:09 pm

Laser induced Fluorescencedetection December 28 - 07:26 am

How to analysis synthesis colorant in food December 30 - 12:47 am

Any body can suggest HPLC METHOD OF ANALYSIS FOR ERYTHROMYCINE CYCLO-11, 12-CARBONIUM BULK AND TABLETS January 20 - 01:33 am

Has anyone created a cleaning validation? January 7 - 06:32 am

Is it retention time depends on concentraion January 5 - 07:05 pm

Diastereomeric peaks in blank injection January 10 - 02:58 pm

Ghost peaks in isocratic reversed phase chromatography June 13 - 10:45 pm

Normal phase HPLC method validation January 12 - 02:36 am

Reverse phase protocol for the HPLC analysis of fatty acids January 14 - 10:18 am

Resolution January 8 - 11:33 am

What is the effect of concentration on retention time January 9 - 03:51 am

Tsk-gel column with high preasure January 18 - 01:57 am

Hplc of sulphonated dyes January 11 - 10:08 am

Preparative LC Information January 16 - 08:27 am

Calibration Curve in different biological matrices January 13 - 03:26 am

Pre-Columns and Guard Columns, When Needed? January 22 - 07:35 am

Search for allicin standard January 20 - 11:44 pm

NO BODYIS LOOKING AS MY ERYTHROMYCINE CARBONICUM QUESTION PLZ ANSWER THIS January 26 - 01:23 am

Negative peak January 22 - 06:44 pm

Negative peak in solvent January 17 - 06:31 am

Pump problem caused by high pressure?? January 18 - 10:39 am

Deproteination By Membrane Filtration January 16 - 11:45 pm

Glucosamine and Ibuprofen HPLC method January 17 - 07:23 am

Calibration for suppressed anion chromatography using a carbonate eluent January 22 - 02:56 pm

Amino Acid Analysis January 31 - 06:48 am

How Many HPLC's Sold Per Year? January 21 - 05:11 am

Easy HPLC pump question January 22 - 09:44 pm

Ultrasounds and hplc columns January 22 - 03:21 pm

Error in Reporting of related substances test January 22 - 06:28 pm

How to make .cdf files with ChemStation ? January 22 - 02:19 pm

Differences among tetrabutylammonium salts July 2 - 07:20 am

Difference between a pre-column and a guard column January 25 - 11:12 am

Negative peaks using tetrabutylammonium August 22 - 07:31 am

Column flushing January 28 - 02:36 am

The effect of Methanol and silica January 25 - 08:33 am

Fosfomycin analysis January 25 - 07:20 pm

Updating January 30 - 06:36 am

Wavelength maximum for assay January 31 - 01:19 am

HP 1100 problem January 30 - 06:29 am

Ghost peak January 30 - 03:53 pm

Baseline problems with Agilent 1100 series RI detector January 31 - 06:02 pm

How to reduce the emulsion after LLE? September 12 - 05:46 pm

PEAK PURITY ANALYSIS USING WATERS 996 PDA July 28 - 12:40 pm

HPLC change August 1 - 11:57 am

HIGH PRESSURE.... July 8 - 07:39 am

Zorbax SB-phenyl column_not easy to work with April 2 - 11:08 am

Neutralization of gentamicin sulphate May 21 - 11:21 am

Streptomycin in Milk August 14 - 08:04 am

Maleic acid derivatives HPLC March 31 - 03:13 am

HPLC sunscreen assay April 15 - 06:06 pm

Ciral analysis of clemastine. July 15 - 06:12 pm

Separation problem December 3 - 03:51 am

Drifting baseline in gradient LC with acetonitrile mobile phase April 1 - 12:00 pm

I need suggestions for a new column! July 30 - 12:38 pm

TLC Solvent System October 30 - 05:32 am

Now here's a tough separation August 22 - 04:38 pm

Chiral Column Library November 8 - 10:43 am

Retention Factor November 13 - 08:21 am

Dionex Summit December 4 - 12:44 pm

Chlorprothixene: Need a sensitive method December 10 - 01:32 am

Manual or Automatic Injector? January 11 - 11:35 am

Fraction Collector January 22 - 01:30 pm

Tubochrom for workstation July 16 - 01:11 pm

Capillary window breaking problem November 22 - 01:55 am

Anyone got a good method for examining porphyrins by HPLC? January 4 - 01:24 pm

Analytical method development May 27 - 08:18 am

Assay Cross Validation January 27 - 10:44 am

Chromatography software January 27 - 10:59 am

Forced degradation/stress study June 23 - 07:28 am

CLC January 2 - 09:18 am

Peak shape bad June 9 - 07:06 am

Which is useful, a quaternary pump or a binary pump? January 9 - 07:50 am

HPLC Calibration April 12 - 08:12 am

S/N ratio January 27 - 12:05 pm

Column failures sources January 7 - 05:58 am

Column Heaters January 7 - 05:50 am

Change in RTs with different columns of the same material and same batch January 4 - 03:54 pm

Columns July 26 - 05:09 am

Ruthenium by ion chromatography April 12 - 03:33 pm

Ethanolamine by IC June 25 - 10:29 pm

Anyone online who want to help me about simple RP/equiqments"problems March 21 - 01:01 am

Isocratic Methods: Pre-Mix Mobile Phase vs On-Line Mixing March 20 - 05:47 am

Phase Collapse March 4 - 12:39 pm

Problem with mobilphase February 21 - 08:55 am

Carryover March 17 - 06:33 am

Method developemnt for peptides of 4 amino acids difference February 21 - 08:35 am

Response change February 15 - 03:16 pm

Standard curve variability March 30 - 08:32 pm

Columns for basic pharmaceuticals March 4 - 01:41 pm

Mobile phase pH February 28 - 09:47 am

Isomer separation February 28 - 09:56 am

Buffer precipitation in HPLC February 28 - 09:58 am

Buffers and volatility April 16 - 05:30 pm

How to find the appropriate void marker? February 15 - 07:59 am

Chemiluminescence detector used for HPLC detection February 21 - 02:05 pm

LC Separation and Detection of alklyamines February 12 - 05:51 pm

Computer assisted chromatography February 19 - 03:39 pm

Kromasil February 4 - 06:16 am

Aspirin November 5 - 07:32 am

Band broadening February 7 - 01:15 am

Used Equipment Advice? Suppliers? March 29 - 09:22 am

SMB chromatography April 18 - 07:55 am

RETAINING POLAR COMPOUNDS February 12 - 08:29 am

Low pressure February 4 - 01:48 pm

Acid-base indicators May 1 - 09:03 pm

Study of peptide degradation in serum by HPLC February 5 - 03:52 pm

Free fatty acid analysis in the presence of lipids February 6 - 12:52 am

SEC/GPC for polylactic acid February 6 - 12:24 am

Clarithromycin Impurities February 6 - 04:48 am

How do organic/buffer affect pKa? April 18 - 09:03 pm

Hydrophilic Interaction Chromatography February 12 - 01:58 am

Retention Time variability in gradient run February 11 - 04:19 am

Fast Flow and Small Columns: Am I turbulent? February 10 - 06:19 pm

Establishing specificity using HPLC methods for excipients June 11 - 04:43 am

Coverting % area to %w/w February 15 - 07:27 am

Gradient vs isocratic chromatography February 21 - 10:34 am

Column diameter February 19 - 10:29 am

Reason for negative peaks in RP HPLC? February 21 - 11:15 pm

HPLC Sample Vials March 1 - 03:48 pm

Protein binds in a weak but not in a strong ion exchanger March 11 - 09:09 am

Increasing baseline drift for samples with little chromaphore February 20 - 01:53 pm

High Pressure Problems February 21 - 08:48 am

Separation of nucleosides, nucleotides and nitrogen bases October 13 - 08:01 am

Portable HPLC May 29 - 02:12 pm

Solanidine February 25 - 07:57 am

Analytical supercritical fluid chromatography (SFC) February 27 - 02:03 am

Problem with absorbtion coefficients March 8 - 12:13 am

Lower detector intensity February 28 - 10:23 am

To analyze Diketones in HPLC March 11 - 09:02 am

Hi pH stable HPLC columns April 17 - 05:45 pm

Problems with Heptane? March 20 - 11:19 am

Advice on software to handle chromatogram? April 3 - 05:56 am

Polydimethylsiloxane (PDMS) using ELSD March 13 - 05:29 am

Protein purification ligands supplier March 8 - 12:49 am

Petrolatum March 8 - 07:23 am

Fronting and "foot shaped" signals March 15 - 02:21 pm

Determination of lactic acid by HPLC June 18 - 12:56 am

Phospholipids analysis in chocolate May 21 - 02:44 pm

Reduction of run time March 13 - 12:22 pm

Passivation HPLC stainless steel March 20 - 04:49 am

Column aging? March 27 - 11:12 am

What a column and mobile phase should be used? March 18 - 03:13 pm

HPLC quantitative analysis March 27 - 11:18 am

Injection volume and concentration March 18 - 03:33 pm

Waters development kit March 19 - 01:07 pm

Caffeine by LC March 21 - 03:24 pm

Calibration in Chemstation March 25 - 06:46 am

PH 5.0 buffer for DEAE SEPHACEL?????????? March 25 - 03:06 pm

Find the analysis method of N-Phenylmaleimide?[941-69-5] !!!! March 21 - 10:32 pm

Non-identical retention times March 25 - 03:42 am

Agilent, Waters and cross control of same (by them or anyone else) March 27 - 11:21 am

Organo metallics April 15 - 05:32 pm

Column Void Volume April 1 - 07:06 am

Size exclusion cartridges for sample preparation March 27 - 06:04 am

Hydrolysis of urethanes March 25 - 07:50 pm

Fluorescent marker April 1 - 01:51 pm

Problem of separation between aromatic polar compounds June 10 - 08:42 pm

Cappilar HPLC April 1 - 09:27 am

FDA requirement for pH of the Mobile Phase April 8 - 11:37 pm

Long equilibration time abd very broad peak on Xterra C18 May 2 - 07:57 am

Cyano and phenyl columns switch from reversed to normal phase April 11 - 08:55 pm

Sticky molecules. How to deal with them? April 8 - 11:55 pm

Base deactivated columns. April 4 - 07:56 am

Sticky molecules. Adsorption or decomposition. April 15 - 06:27 pm

Column of diastereomer separation? April 5 - 01:58 am

Dalton units April 9 - 11:56 pm

3-Chloroperbenzoic Acid [937-14-4] March 25 - 11:11 am

Asymmetry Question April 10 - 10:01 pm

Calibration Curve April 22 - 05:06 am

Oxcarbazepine by hplc May 2 - 11:38 pm

Unknown Peak probably from gradient system May 23 - 08:09 am

Chloride Peak Tailing/Asymmetry April 18 - 02:17 pm

N-octadecylphosphonic acid ?? April 19 - 08:20 am

HPLC Miniaturization April 12 - 08:03 am

Use of graphite columns April 12 - 06:27 pm

Column Heaters April 17 - 07:41 am

Ion Suppressor April 22 - 11:44 am

Resolution, gradient, HP Vs Waters 2690 April 23 - 06:44 am

Re-validation of assay method April 20 - 12:37 pm

Stress study April 18 - 08:52 am

FPLC ?? August 5 - 11:56 am

Dissolved oxygen and electrochemical detection April 22 - 02:34 pm

Acid Interaction May 17 - 03:28 am

Retention time drifting with ion-pair chromatography May 22 - 01:07 am

RENTIVITY PROBLEAM April 22 - 06:05 am

Inconsistent SPE results May 10 - 03:12 pm

Calibration curve II: to weight or not to weight April 26 - 05:20 am

How to isolate Vitamin E and Carotene using Chromatography April 23 - 08:55 am

Relative Response Factor May 10 - 02:57 pm

Is there any way to put Millinium results into Excel correctly? April 25 - 07:18 am

Buffers April 30 - 11:24 am

HPLC make up April 26 - 12:02 pm

HPLC April 23 - 10:55 pm

To remove CH2Cl2-originated peaks ? May 2 - 01:38 am

Microbore LC Columns May 1 - 10:51 am

Which columns are best for isomer separation May 23 - 10:56 pm

Carbohydrate analysis - split peaks May 2 - 05:31 am

I need a method to determine fatty acids with RPLC May 6 - 01:41 pm

Any opinions about HPLC Troubleshooting courses? May 8 - 10:17 am

Mechanical resistance of silica ... May 4 - 08:50 am

HPLC of sugar phosphates May 2 - 06:02 am

Dansyl Chloride Deriv. for Amines May 3 - 08:45 am

Water on Column -- Problem? May 3 - 12:17 am

Instrument logbooks May 6 - 11:34 am

HIGH SPEED HPLC MICROBORE COLUMNS May 7 - 06:53 am

How to make PEAKS ATENUATION ? May 6 - 07:39 am

Waters 2690 degasser module May 13 - 01:13 am

Ask help for Analysis of Alanine and Ammonium May 7 - 12:30 am

Inorganics on HPLC May 9 - 08:13 am

PKa logP softwares April 25 - 11:57 pm

Connect a integrator to a PC April 19 - 10:24 am

Anyone has good experience with ELS? June 29 - 06:07 am

ASSASY OF COMPOUND HAVING POLYMORPHS May 13 - 07:00 pm

Tom please check out my previous talk on isomer separation May 4 - 08:26 am

Detection of microgram quantities of 3-dimethyl-1-phenyl-2-butanone May 5 - 06:12 pm

Ways to prepare a buffer? May 13 - 01:37 am

Waters Alliance & Millennium32 Problem May 8 - 05:52 am

Separation of polar isomers by reversed phase HPLC May 17 - 05:18 am

Calculating peak area on basis of the absorption coefficient May 16 - 12:49 am

Shimadzu equipment May 8 - 04:57 pm

Limit of Detection (LOD)/Limit of Quantitation (LOQ) May 9 - 10:45 am

HPLC Column choice May 12 - 07:22 pm

How to determine the consentration of creatinine in urine? May 30 - 12:10 pm

ESI sensitivity and mobile phase May 9 - 03:14 pm

CHOICE OF DIODE ARRAY DETECTORS May 9 - 02:34 pm

How to recognize effects of residual metals May 18 - 03:49 pm

Liranaftate HPLC method May 12 - 07:25 pm

Postcolumn reaction coil May 14 - 04:11 am

Separation of glucose and sodium chloride May 30 - 04:26 am

ABS May 21 - 05:09 am

Supplier suggestion for solid HPLC vial caps needed May 14 - 05:11 am

Accuracy May 14 - 01:31 am

Column bubbles May 17 - 01:43 am

Difference on PDA detectors from different manufacturers May 22 - 06:47 am

CN or C5 columns stable at pH > 9 May 18 - 03:57 pm

Disappearing protein May 17 - 03:22 am

ChromSword software May 20 - 02:00 pm

Method for Glycerol (~20%)in Skin Cream? June 19 - 09:34 pm

Whither HPLC? May 27 - 03:52 am

Agilent 1050/1100 Chemstation software glitches May 21 - 10:24 am

Model for ELSD May 21 - 07:23 pm

Linear diastereomer separation May 23 - 05:19 pm

Reducing column dimension June 5 - 08:46 am

Assaying Sodium Metabisulphite by HPLC May 24 - 10:18 am

Agilent ChemStation USP system suit calculations May 29 - 01:33 am

Degassing May 23 - 09:14 pm

Sample carryover associated with autosampler June 4 - 02:06 pm

Hp Chemstation Software(G2180AA) for HP 3D CE system? May 24 - 09:29 am

HPLC Method Development April 26 - 07:05 am

Contamination from caps May 27 - 06:28 pm

Quaternary ammonium salts by HPLC June 3 - 08:30 am

Sensitivity criteria for PDA-detector May 26 - 04:48 pm

Deggasing : Helium or vacum membranes May 29 - 03:13 pm

CE handbook May 28 - 09:57 am

Problem Negative peak in RI detection June 11 - 12:00 am

Agarose Bead Dissociation May 29 - 11:31 am

Solubility of 25mM phosphate at pH 5.5 mixed with 30% methanol and 10% THF June 2 - 02:31 pm

Buffers in mobile phase June 2 - 02:34 pm

Method to Measure THP acid in THP anhydride June 1 - 04:32 am

Calculating peak area via absorption coefficient May 7 - 07:25 am

Cefixime June 1 - 05:23 am

GLP/GMP enviroment June 5 - 06:43 am

Number of significant figures to use June 5 - 11:27 am

Organic modifier strength June 11 - 01:32 pm

HPLC mobile phase life June 7 - 06:12 am

Method Validation June 7 - 08:36 am

Analysis of Methyl and Propyl Paraben in Shampoo June 7 - 02:00 pm

Thc removal from hemp oil June 18 - 12:01 am

UV cutoff table June 10 - 02:28 pm

HPLC column June 9 - 07:18 am

HPLC column filing cabinets June 7 - 11:03 am

Chiral separations June 13 - 08:47 am

Collapse of HPLC column June 29 - 05:53 am

Cr-picolinate hplc method of analysis June 21 - 10:19 am

PEO on TSK gel June 18 - 11:41 pm

USP Heavy metals June 12 - 05:24 am

Internal standard June 19 - 06:01 am

Will flow rate affect the sensitivity? June 13 - 11:16 pm

HPLC method for determining solubility of organic compounds June 13 - 04:02 am

Millennium32 version 4.0 June 13 - 02:19 am

Relationship between column i.d. and injection volume June 14 - 07:59 pm

HPLC PEAK PURITY June 30 - 12:41 am

Placebo solubility June 19 - 08:18 am

Glycophosphate? June 19 - 10:26 am

What is liqiud scintilation? June 19 - 10:42 am

HILIC June 24 - 02:57 pm

Empty PEEK columns Suppliers June 21 - 12:15 am

Moisture content in bonded hydrated molecules June 24 - 10:45 pm

HILIC SPE cartridges June 25 - 07:39 pm

Guard column validation June 25 - 09:14 am

Formol titrations June 26 - 10:34 pm

Retention Time Shifts June 30 - 06:32 pm

Capillary 2D liquid chromatography June 5 - 08:31 am

Peptide separation and biological activity June 28 - 08:11 am

Radio Detector for HPLC February 28 - 08:54 am

John W. Dolan's "LC Troubleshooting" predictions (latest LCGC NA, 20/3, 2002) March 19 - 08:29 am

Pneumo-hydraulic hplc pump wanted April 24 - 10:35 am

ASCII to AIA file conversion March 1 - 02:02 pm

JMBS March 19 - 04:42 pm

PH 1.00 buffer for calibration April 13 - 02:52 pm

Urgent! May 15 - 09:48 am

Wanted: supplier(s) of empty plastic chromatography cartridges. June 21 - 07:55 am

Glass tubes/caps May 3 - 02:21 pm

How a drift baseline affects integration July 22 - 06:53 am

Buying An HPLC (for newbies) July 16 - 02:34 am

Chromatography Software May 28 - 09:41 pm

Improving post-column reaction Flourescence reproducability July 25 - 12:23 pm

HPLC Column Log system for the Lab July 20 - 05:10 am

Low pressure mixing - TFA Buffers July 17 - 02:34 pm

Use of n-Propanol in RP-HPLC mobile phases! July 3 - 06:17 am

Input signal out of range July 24 - 11:51 am

Columns for underivatized amino acids analysis April 10 - 03:02 am

DNA analysis by RP-HPLC August 26 - 03:29 pm

UV_VIS Detector flowcell July 3 - 01:49 pm

Bandwidth in HPLC detector May 11 - 07:56 pm

MeOH vs MeCN? July 10 - 11:09 pm

Mass Balance requirements for method validation July 2 - 12:25 am

Any HPLC manufacturers based in Spain? July 4 - 12:03 pm

Networked Chemstation July 20 - 01:46 pm

Column Log July 9 - 07:43 pm

Two different lines from the pump system July 1 - 07:41 am

Peak splitting problem July 21 - 01:52 pm

Signal to Noise July 3 - 05:04 am

Quantification of TRICLOSAN in personal care products June 26 - 07:04 am

SOP-General criteria for the suitable application of HPLC methods July 2 - 08:56 am

Method Transfer July 2 - 03:17 am

Chiral Column for Levofloxacin July 2 - 03:49 am

21CFR Part 11 template July 20 - 02:02 pm

Polysiloxane-based anti-foaming agents in beverages July 10 - 01:15 am

Peak to valley ratio for resolution July 3 - 11:45 am

Suppliers for polymer-based weak cation exchange SPE cartridges July 5 - 11:11 am

Suitability test before and after the analysis July 9 - 07:29 pm

Min pressure setpoint for pumps July 11 - 05:44 pm

Stability Analysis of Dexamethasone with RP-HPLC July 14 - 03:43 am

HP1100 UV-detector at 200nm -> collapsed response after changing lamps July 16 - 08:24 am

Unknown Peak in LC system (UV detection) July 23 - 05:37 am

Please help with my impurity linearity July 15 - 08:37 pm

Doxazosin Assay, Dear Friends July 25 - 09:12 am

75 um ID columns July 15 - 08:55 pm

APROPIATE BUFFER July 18 - 03:22 am

How to prepare 20% methanol in water? July 17 - 06:48 am

Column clean up help!!!!! August 8 - 03:02 pm

Six Sigma and Analytical Testing July 21 - 08:46 am

L53 Columns July 14 - 03:52 am

Injection Accuracy July 16 - 02:42 pm

HPLC-fluorimetric detector-time zero July 16 - 02:34 am

GPC Mobile Phase Modfiers July 16 - 05:14 pm

Increase in RT when I increase temp July 17 - 11:35 pm

SPE Manifold July 17 - 08:20 am

Copper detection by ion chromatography? July 19 - 04:39 am

Detection of imidazole by hplc April 2 - 04:41 am

Cl in silica based slurry July 18 - 11:49 am

C18 and octadecyldimethylsilyl July 24 - 02:49 am

Haddad Transfer Equation July 18 - 05:25 am

METHOD FOR VIT B12 April 30 - 12:03 pm

Reloading of HPLC millennium software July 22 - 11:51 am

Please help me solve my baseline problem July 25 - 01:10 pm

Irregular peaks in the baseline during gradient August 24 - 06:31 am

Estimation of a retention time when changing the mobile phase July 19 - 06:43 pm

Method for determination of sugars and PEG by HPLC/IC July 22 - 12:49 am

Method for DDBSA July 19 - 12:57 pm

DRI detector and negative peak August 5 - 06:52 am

Quantitative detail - standard injection (single or multiple?) August 8 - 05:11 pm

THF compatible tubings July 29 - 03:12 am

Polar dirt stuck in C18 column? July 29 - 06:46 am

CuSO4 buffer usage July 27 - 08:07 am

Improving Tailing Factor August 1 - 08:50 am

Fixing no. of theoritical plates July 30 - 06:21 am

PEG-8000 August 11 - 04:10 am

HPLC EVALUATION August 21 - 08:57 am

KH2PO4 BUFFER SOLUBILITY August 7 - 07:00 am

Diol Column August 8 - 08:40 am

Denaturing HPLC August 8 - 03:53 am

Fast screening methods and monolithic columns August 5 - 11:11 am

Hyaluronic Acid Analysis February 11 - 09:37 pm

Vial septa crumbs from injector valve August 8 - 11:11 pm

Beckman System Gold HPLC system August 12 - 09:44 am

Theorical questions August 9 - 05:12 pm

Analysis of Fmoc-Amino Acid August 9 - 01:29 pm

RAM columns August 14 - 04:48 am

How to separate L-cysteine from L-cystine for HPLC/UV analysis August 12 - 09:55 am

Accuracy for a method when no Standards are Available August 15 - 09:38 am

Area reproducibility August 16 - 05:22 am

Cation Exchange column August 20 - 01:27 pm

Maximum column temperature August 22 - 03:43 pm

Biphosphonates August 17 - 10:18 am

Where to lease a HPLC? August 19 - 08:06 am

Flow splitter calculation August 22 - 03:50 pm

RP HPLC METHOD OF ANALYSIS FOR SERRATIPAPTIDASE August 29 - 11:39 am

Ultron ES-pepsin column August 20 - 12:22 pm

Basic integration theory August 21 - 05:38 pm

Peak Tailing .... August 24 - 12:22 pm

System suitability test August 29 - 06:13 am

Biotrap column August 30 - 10:47 am

LC System Clean up August 29 - 10:49 am

PH in RP Mobile Phases August 30 - 07:03 pm

Looking for an old Paper August 21 - 05:16 am

Preparative HPLC August 21 - 01:17 pm

Help with normal phase vs reverse phase explanation August 5 - 09:33 am

Petro industry application July 21 - 04:18 am

Column rinsing in reverse direction August 31 - 07:13 am

Morphine in injectable August 14 - 05:46 am

Separation according to size August 16 - 03:21 am

Void Volume Determinations August 20 - 02:05 pm

Separation of NaCl January 20 - 10:15 pm

Determination of cellulose in liquid products January 23 - 08:32 am

Oligomeric Proanthocianidynes (OPC's) from Pine and Grape March 3 - 08:21 am

HITACHI L9100 March 6 - 04:39 am

Vial crusher March 8 - 07:45 am

HPLC-colum? September 24 - 04:42 am

What is a good antibacterial buffer additive. September 4 - 11:39 am

Organic Acids by RP-HPLC June 14 - 02:25 pm

Silanizing Glassware November 6 - 07:50 pm

Mechanisms of Chromatographic separation December 27 - 10:48 am

UV absorbance November 6 - 07:53 pm

Stavudine impurities method September 19 - 12:13 am

Hplc analysis of amino acids using uv-vis detector September 27 - 02:59 pm

Void Volume November 6 - 07:53 pm

Electrochemical detector December 20 - 06:44 pm

HPLC-ECD- using ion pair reagents for catecholamines November 6 - 07:45 pm

HPLC Performance December 19 - 08:57 am

Laser Induced Fluorescence Detection November 7 - 05:51 am

Mobile phase for lenthy analysis December 26 - 09:13 am

LC Method November 6 - 07:56 pm

Gradient to isocratic November 6 - 07:30 pm

DETECTION OF AMINO ACIDS IN SMALL SAMPLE VOLUME AND fmol range October 22 - 04:29 pm

THF containing mobile phase September 23 - 08:14 pm

Clinical HPLC September 6 - 08:44 am

Chiral Separation September 23 - 02:15 am

DMSO on Reversed phase September 24 - 02:33 am

Need an article, please help March 25 - 10:43 pm

Need some guidelines June 5 - 12:41 am

Negative peak problem in RI detector September 12 - 07:17 am

Help DAD Detector Baseline September 5 - 08:37 am

High pressure during automated set of runs leads to system shutdown November 29 - 01:30 am

Peak purity vs. Assay value September 16 - 07:33 am

Iminium Salt September 6 - 12:06 am

Animal Feed sample preparation November 6 - 07:38 pm

Licopene determination and its epoxides September 13 - 11:18 am

Storage of THF - neat vs. mixed with ACN September 13 - 08:34 am

Acrylamide April 7 - 04:10 am

Water in Silica column February 26 - 06:52 am

Lost peak in HPCEC September 16 - 08:33 am

Calling the chromatography gods to settle an inter-lab dispute September 12 - 03:22 pm

Storage of anion exchange column September 12 - 06:29 pm

Prediction of retention from chemical structures September 14 - 02:15 am

Glucosamine November 6 - 07:27 pm

CMC in laundry powders November 6 - 07:23 pm

Theories relating chemical structures with retention in RP-HPLC June 2 - 02:03 pm

Use of pKa value September 26 - 09:15 am

LC method for quaternary amines September 17 - 04:30 am

New HPLC columns Qualification procedure September 24 - 05:31 am

21CFR Part 11 question September 24 - 02:59 pm

RP column vs slurry packed cartridges September 17 - 09:59 am

Affinity chromatography the hard way? September 24 - 04:18 am

Elusion sequence September 19 - 12:08 pm

Aspirin USP assay method? October 10 - 02:44 pm

Analysis of peptides by HPLC using PDA detector September 20 - 12:11 am

HPLC of mixture of HC and FA September 21 - 09:20 am

Chiral isomer separation September 20 - 05:04 am

Determination of EDTA Content in ppm or ppb levels September 24 - 09:35 am

Polymorphs analysis by HPLC September 24 - 12:12 pm

Peak area units for linearity graph April 28 - 06:03 am

Dionex AND QC October 2 - 07:21 am

Looking for a hplc column September 24 - 02:32 pm

Standardize a standard? September 25 - 05:27 am

Amino ethyl ethylene urea September 25 - 09:59 am

Wavelength adjustment? October 2 - 07:40 am

2D chromatography run times September 29 - 02:26 pm

Separation of mixture b-cyclodextrin complex/free drug September 26 - 05:40 am

Inertsil Bioptic AV-2 September 26 - 04:06 pm

Bromide ion identification October 4 - 01:05 pm

Enantiomeric separations September 30 - 08:07 am

Can silica induce changes to samples? October 1 - 11:51 pm

Wavevy baseline October 3 - 08:15 am

Peptide/Protein Deconvolution Software August 22 - 10:08 am

HPLC methods for determining log P October 9 - 04:49 am

Unidentified split peak question December 24 - 07:07 am

Validation methedology for precision study October 10 - 06:31 am

Changing response factors October 4 - 08:58 am

Area counts changed with different flow rates October 7 - 02:47 am

Standard curves October 11 - 07:46 am

How to export the data from Star LC Workstation 5.0 into ASCII file October 7 - 05:38 am

Rotational isomers in RP-C18 analysis October 9 - 11:02 pm

Polar embedded phases for Nucleotide base/nucleoside analyses October 22 - 11:20 am

How to calculate 95%Confidence curve? October 4 - 06:01 pm

Triglycerides October 9 - 05:10 am

Molecular Sizer? October 7 - 11:29 pm

TLC October 5 - 03:01 pm

Botox standard October 7 - 03:48 pm

Guard Columns October 9 - 12:40 pm

Paramertrs for validation of dissolution method. October 1 - 11:12 am

Revalidation on tightening of the specification October 11 - 05:30 am

Analysing Betaine content in beet molasses October 8 - 09:28 pm

Cinitapride tablets / quantitation by HPLC / low recovery October 11 - 05:52 am

Cross-validation October 9 - 10:02 am

Polypropylene vs. glass vials October 10 - 02:15 am

Pressure spikes October 9 - 11:32 pm

HPLC purification of DNA oligos (35-mer) October 11 - 11:16 am

Method Development vs. Method Validation October 14 - 11:01 am

Evaporative Light Scattering Detectors November 7 - 05:25 am

Unexplainable Wavy Baseline Without Flow January 30 - 03:52 pm

Unexplainable problem with Specificity October 14 - 06:57 am

Dichlorobenzoic Acid Isomer HPLC October 18 - 12:09 pm

LOQ and LOD calculation October 14 - 09:01 pm

Is ChemStore any good? October 28 - 10:12 am

LC Radioactive detectors October 14 - 10:22 pm

Could you said how to separate sugar fatty acid esters by chromatography? October 21 - 10:54 am

2-Phenoxyethanol October 18 - 10:46 am

Hydroxyzine November 1 - 03:28 pm

Dissolution Profiles - straw poll October 24 - 01:54 pm

Related substances in Rocuronium bromude - looking for HPLC method October 21 - 10:11 am

Variable retention time October 23 - 04:04 pm

Amino Acid Separation October 23 - 06:50 am

LIMS systems October 28 - 04:22 pm

Help Trace Analysis October 21 - 02:16 pm

Hplc method for Trimebutine maleate in tablets. October 21 - 10:47 am

Acceptance Criteria for analytical Method Validation October 24 - 06:33 am

Strange Spectra October 23 - 11:03 pm

Niacin and lovastatin February 21 - 12:27 am

System Suit- Detector on/off? October 30 - 02:01 pm

Excipient Studies? October 24 - 01:10 pm

Help about Impurities October 24 - 02:17 pm

Removal of ftalates from Milli-Q water October 26 - 07:35 pm

Peak symmetry factor for drifting baseline October 27 - 04:51 pm

Method development language (MDL) for auotsampler October 24 - 10:10 am

Shimadzu users: asking about SCL -10A controller October 27 - 05:50 pm

Agilent 1100 and Firmware v 5.01 October 25 - 11:30 am

Theoretical t0 - how is it derived? November 1 - 04:19 am

Problem with Waters Empower/Millinium software November 27 - 01:26 am

Chaotropic and Lyotropic agents October 31 - 03:02 am

PDA detectors November 4 - 07:16 am

Concentration dependant retention times? November 1 - 04:13 am

Waters AMDS November 12 - 05:26 am

HPLC problem with PAH's analysis after use of triethylamine buffer November 4 - 02:12 am

Peak Purity on DAD-Detector October 30 - 11:47 am

Buffers problem November 4 - 03:43 am

Chemstation problem - disappearing files October 31 - 04:02 am

Overloading November 1 - 11:45 am

CN phase stability November 13 - 01:12 pm

Sodium omadine assay November 1 - 09:00 am

Diazolidinyl Urea (Germall2) November 1 - 06:10 pm

Alendronate Sodium PF dissolution November 12 - 05:15 am

Specific recovery question November 14 - 12:38 pm

Selectivity verse Specificity November 5 - 07:59 am

Have anyone an idea about TSK-Butyl? November 8 - 11:00 am

2 deoxy d glucose / glucose separation November 14 - 07:24 am

2-methyl-4-isothiazolin-3-one (MIT) November 8 - 12:33 pm

Method for Acetylsalicylic Acid Tablets November 18 - 05:55 pm

Acetic-, propionic-, butyric- valeric acid with HPLC April 14 - 04:48 am

QTOF November 13 - 08:18 am

HPLC mobile-phase November 18 - 06:12 pm

Universal test for RP supports November 21 - 03:25 pm

Sodium docusate as mobile phase November 27 - 10:32 pm

Why do retention times of isomers correlate with their boiling points? November 15 - 06:44 am

Problem with column dewetting April 25 - 11:18 am

Recovery problem November 22 - 06:23 am

Vitamins November 19 - 07:27 am

Dual active assay ruggedness issue December 3 - 03:55 pm

Fatty acids by GC or HPLC? November 20 - 07:09 am

Hplc retest / failures June 5 - 09:08 pm

NH4H2PO4 pKa November 27 - 11:53 am

SFC check valve availability November 21 - 09:34 am

A Persistent Ghost peak(need help) November 23 - 05:12 am

Acetic acid tail in FFAP column November 24 - 08:49 am

About pKa's, Acids and Bases March 27 - 07:18 am

Tamoxifen stereoisomer separation November 25 - 10:40 am

Permiation by liquids November 25 - 03:48 am

Dead time and chaotropic modifiers December 12 - 07:29 am

Can I use my Waters 625 LC system with a LC-318 Octadecyl column? November 28 - 12:57 pm

EDC (or EDAC, Ethyl-3-(3-dimethylaminopropyl)-carbodiimide November 26 - 12:25 pm

Relative retention times(RRT) fluctuation November 27 - 11:58 am

Salicylic acid and betamethasone dipropionate November 27 - 02:43 am

Routine system performance December 1 - 01:28 pm

Choice of optimum extraction solvent December 7 - 07:41 am

Any trick to derivatize and analyze monophospahte December 5 - 04:45 pm

EP assay for Vitamin A December 5 - 03:08 am

Adsorption in plastics December 12 - 02:16 am

Which column? April 6 - 06:13 am

Contract laboratory for Nitroso compounds analysis December 5 - 12:06 pm

Cerity March 13 - 08:08 am

Commercial source of human plasma for validation? December 10 - 07:06 am

Bromopride December 6 - 08:01 am

FDA 483 letters and reviews December 9 - 12:33 pm

After flushing column with very big flow rate, what should I do next? December 10 - 05:35 am

How to maintain HPLC column December 9 - 05:59 am

Method for Bromopride Assay December 9 - 06:19 am

Ion pair reproducibility December 17 - 05:44 pm

HI 1200 upgrade for 7673 ALS August 13 - 08:07 am

HPLC analytical method of azithromycin May 17 - 12:26 pm

Bromopride Method December 9 - 09:58 am

Prednisolone December 13 - 06:07 am

Gilson Prep. Instrument December 10 - 05:18 pm

Bioanalytical question December 12 - 08:27 am

Information about capillary HPLC December 18 - 01:54 pm

DIY Affinity columns December 20 - 02:07 am

Qualification of columns December 18 - 06:27 am

Ammonium Carbamate December 16 - 12:35 pm

ActiveX Chart Component for AIA/netCDF files December 16 - 09:52 am

Metal-chelater complex separation from original chelater in LC December 20 - 11:04 am

Validation of USP methods December 27 - 01:27 am

LC to "Dye" for! December 20 - 11:37 am

Substantial decrase of benzo(a)pyrene peak in PAH analysis December 20 - 01:38 am

PH of a compound December 24 - 02:46 pm

Problem of Peak splitting December 29 - 07:32 am

Waters Empower Software January 9 - 07:07 am

HPLC software validation June 23 - 07:18 am

Prednisolone - related substances December 1 - 05:04 am

Peak "Fronting" November 17 - 06:09 pm

AllTech Rocket Columns December 11 - 11:59 am

Capillary Electrophoresis December 17 - 03:24 pm

Niacin and lovastatin April 27 - 04:50 pm

Sample treatment for determination for vitamins in HPLC June 22 - 01:26 am

Problem with high preasure in a microbore column December 4 - 12:10 pm

Looking for some schematics, hope someone can help September 21 - 01:00 am

ION CHROMATOGRAPHY July 10 - 10:19 am

Standard curve in catecholamine analysis March 3 - 04:53 pm

Phenoxyethanol March 29 - 07:18 am

Percent recovery of an analyte May 31 - 08:44 am

Hydramethylnon April 29 - 11:10 am

Tailing and Fronting May 19 - 01:04 pm

Delay volume in HPLC July 29 - 04:37 pm

KH2PO4 BUFFER SOLUBILITY August 14 - 04:30 am

HPLC equipment. June 18 - 07:59 pm

TLC September 26 - 05:48 pm

Validation for antioxidant methods of analysis . September 14 - 03:21 pm

Lincomycin October 23 - 11:45 am

Common Lab Practice - Number of Injections November 21 - 04:26 pm

Setting spec's for assymetry vs H/W ratios November 25 - 03:40 pm

Spectroscopy Question! November 27 - 01:01 pm

Runtime21 July 24 - 11:26 am

New online electrophoresis forum August 22 - 07:17 pm

HPLC Web Directory/Search August 8 - 09:23 am

T.DBL August 8 - 11:56 pm

Please help me January 20 - 04:39 am

A plea... More information, please August 30 - 07:05 pm

CE equipment September 15 - 10:53 pm

Chromatography forum October 11 - 08:14 am

Help me find it!!!! December 7 - 02:12 pm

Humour and Analytical Chemistry December 23 - 05:07 am

Denaturing HPLC for DNA analysis (Wave system) June 5 - 12:15 pm

Diastereo isomers By HPLC June 18 - 08:52 am

HPLC method of DONEPEZIL (ARICEPT) June 21 - 12:31 am

Ask for some suggestions on capillary HPLC January 17 - 12:05 pm

UPS for LC GC and other lab instruments July 14 - 11:46 am

Specificity Question April 5 - 03:59 am

Gradient methods July 30 - 05:12 am

GLP vs non-GLP January 10 - 07:43 pm

HPLC method for androsterone and related substances January 3 - 12:19 pm

Desalting by HPLC January 9 - 01:13 am

What can I use instead of Hexansulfonic acid Sodium salt ? January 6 - 10:50 am

High Pressure versus Low Pressure Mixing January 11 - 07:15 am

TLC Records January 8 - 01:21 am

Low pressure gradient hplc vs high pressure gradient - advantages & disadvantagess January 9 - 08:04 am

Column for substituted saccharids January 7 - 01:38 am

ELSD detection repeatability June 3 - 01:15 am

For fast analysis what column shell I buy, help needed January 13 - 12:41 am

...seperation of highly hydrophobic protected peptides... January 8 - 08:48 am

Stabilization of a strong anion exchange column January 8 - 11:44 am

HILIC column ok with TFA? January 14 - 04:17 pm

Metformin +gliclazide hplc method of analysis May 6 - 08:13 pm

Equivalency vs. Validation March 6 - 05:42 am

Small particle-Large pore HPLC columns April 6 - 04:10 pm

THEORETICAL PLATES January 22 - 03:54 pm

Response factor for Refractive index detectors January 21 - 01:02 pm

HPLC Flow Rate Gradient January 14 - 04:38 pm

Coupling efficiencies and HPLC analysis of Oligonucleotides January 14 - 05:42 pm

Is it posible, Solvent Peak response change due to difference in concentration of sample ? January 22 - 04:29 am

Performance check for Prep.HPLC January 15 - 02:53 am

How much is a new HPLC ? January 17 - 02:54 am

Polysorbate 80 Analysis June 12 - 09:01 am

Validation of Karl Fischer Method for the analysis of Active Drug Substances January 21 - 01:58 pm

Method for the detection of certain Azocolourants in Dyestuff January 20 - 07:59 pm

Ballpen color of signatures in GMP/GLP enviromment January 22 - 07:30 am

Remove proteins from HPLC column January 20 - 11:22 pm

Improving Solution stability of a platinum complex in water for HPLC analysis January 20 - 04:41 pm

Any idea how to quantify hydrogen peroxide and/or urea peroxide (carbamide peroxide) January 20 - 07:53 pm

How to separate NaCl after ion exchange chromatogram? April 9 - 04:01 pm

How to separate NaCl from desired substance after ion exchange column? January 22 - 05:32 pm

Ion Exchange Theory with Resin January 22 - 11:04 am

Different types of membrane filters January 23 - 04:54 am

Cetylpyridinium chloride January 23 - 05:34 am

Equivalency of Column Test procedures January 31 - 01:24 pm

Diastereoisomers in a chiral column January 31 - 03:33 am

Autosampler contamination February 4 - 12:29 pm

Sample mass February 17 - 07:50 pm

HPLC vs CZE vs CEC May 19 - 10:50 pm

Changing retention times of amino acid analysis with a modified Waters AccQ tag method February 14 - 07:10 am

What are the possible causes of shoulders peaks and splittin? April 22 - 06:50 am

A method of itraconazole February 10 - 07:00 am

Two horrible peaks with the same spektrum - Ru dye komplex hplc February 7 - 01:21 am

DEGRADATION of ANALYTE by Acetonitrile HPLC grade May 30 - 05:27 pm

Ion exchange Resin Suppliers February 21 - 10:28 am

Diode array spectra structure analysis June 27 - 02:08 pm

Filtered versus unfiltered mobile phase February 7 - 10:42 am

Has anyone tried USP 26 Isoniazid Injection ? February 5 - 11:29 am

Difference in tailing factors in the peaks of sample and standard. February 6 - 08:57 am

Norethinodrel Degradation February 6 - 01:32 am

Shifting retention time of strongly retained impurities February 6 - 11:40 pm

Shift in Retention Time in the same run February 11 - 12:36 am

Extraction of compound from lymph fluid February 16 - 02:44 pm

CPC analysis February 10 - 12:41 pm

High Presure With Buffered Solutions February 15 - 12:27 pm

Problems with a method February 13 - 05:11 pm

IC jagged peak February 12 - 09:30 am

UV detector cell inlet tube blocked February 17 - 11:38 am

Need to make an inject start trigger February 18 - 02:31 am

20mM phosphate precipitates in 80% ACN??? February 13 - 05:05 pm

Dioctyl Sulfosuccinate February 21 - 05:52 pm

Fluorescence Detector February 18 - 02:32 pm

RPLC separation question February 15 - 10:34 am

UV lamps February 17 - 03:59 pm

Retention times of benzodiazepines February 16 - 02:51 pm

Structure of HPLC equipment components in power point presentation February 17 - 11:27 pm

Solvent/Column question March 29 - 04:36 am

Baseline Drift during gradient elution February 19 - 04:09 pm

Indirect UV-Detection AND Alkylamides February 20 - 06:01 am

Unstable Retention Times for Folic Acid? February 20 - 11:29 pm

PROTEIN DENATURALIZE IN BLOOD SERUM February 20 - 11:39 pm

1050 Degasser pump diaphragm February 20 - 06:35 am

SPE for benzo[a]pyrene February 20 - 03:51 pm

Kratos Spectroflow 773? February 25 - 11:58 am

Fenofibrate February 24 - 10:10 pm

How determine the % ion pair reagent to use February 26 - 11:57 am

Linearity: Calculations February 26 - 12:01 pm

Theophylline analysis February 27 - 04:20 am

Conditioning step in SPE Methods for blood samples. February 27 - 03:12 pm

Cromolyn sodium HPLC *searching article* February 27 - 11:21 am

Dissolution Tests Media February 28 - 07:55 am

Neutralization of gentamicin sulphate June 10 - 05:32 am

Quantitation of Matrine and Oxymatrine?? June 12 - 07:43 pm

Purification of an glycoprotein, which is present in proteinfree medium June 26 - 12:35 am

Dissolution Medium selection for practically water insoluble drugs July 5 - 09:20 am

I have a retantion time drift problem ...... July 14 - 11:03 am

How to analyze N,N-diethylammonium N,N-diethyldithiocarbamate using HPLC? July 29 - 04:37 pm

Ideas for alternative to triethylamine (TEA) in mobile phase July 31 - 05:58 am

Phosphalipid analysis August 1 - 07:27 am

HPLC result variation. September 5 - 07:42 am

VALIDATION OF HPLC March 26 - 04:43 am

Role of Triethyl amine September 6 - 05:44 am

Specificity-Fluore detector September 29 - 10:21 am

Benzoic acid May 26 - 01:16 am

Organic salts April 28 - 12:35 pm

Column packings June 7 - 10:37 am

Biological samples March 31 - 06:45 am

SPE for Drugs in blood plasma April 24 - 04:20 am

Separation factor and theoretical plate calculations for gradient mode March 6 - 12:34 pm

HPLC Analysis of Cu(I) complexes March 4 - 09:48 am

Pore size in RP Chromatography March 3 - 05:23 pm

Xterra alternatives March 5 - 06:32 am

Help in HPLC pump malfunctioning part identification June 19 - 08:23 pm

Strategies for HPLC method development March 7 - 02:02 am

HPLC method validation March 4 - 08:54 am

Column for derivatized amino acid separations March 4 - 11:50 am

Apparent pH of mixed mobile phase March 6 - 09:19 pm

Integrated B1, B2 and B6 analysis March 8 - 10:48 am

Method Validation - Acceptability of Parameters (Ex: Linearity) March 7 - 08:08 am

Post-column OPA reaction failure with primary amines March 18 - 09:20 am

Baseline problems with ECD March 17 - 12:07 pm

Shimadzu software June 12 - 12:58 pm

Different System behaves differently March 17 - 12:15 pm

Heterocyclic hydrocarbons analysis March 17 - 09:58 am

Life of rotor seal March 14 - 03:02 am

IPC and Gradient Elution March 17 - 09:06 pm

Accuracy and precision for a biological assay March 14 - 01:31 pm

Accuracy and precision for a biological assay March 17 - 02:37 am

Oxalic acid retention May 9 - 02:02 am

Phosphenates analysis March 17 - 11:53 am

Pack your own column March 20 - 10:33 am

Flow Rate effect March 28 - 03:12 pm

Interactive pump control with Class-VP software March 18 - 10:17 pm

Problems with Folic analysis March 21 - 03:06 pm

Shifting baseline after first analyte peak June 15 - 12:29 am

Prep Chromatoghraphy April 3 - 07:57 am

Enantiomeric separation of chiral metal coordination polymer March 21 - 02:54 pm

Venom analysis March 25 - 05:43 am

Blending solvents with RI detection March 24 - 10:24 am

Bisamino-PEG conjugates separation ? March 24 - 08:10 am

How to obtain free bases by prep. LC? March 25 - 03:57 pm

Method Validation for impurity estimation in formulation March 25 - 05:35 pm

Pigments March 25 - 02:44 am

PREPARATIVE CHROMATOGRAPHY March 29 - 09:59 pm

SPE cartridge... allow to dry? March 31 - 06:20 pm

How to define raw data March 27 - 07:06 am

Suitable compound for the determination of the dead time March 27 - 07:16 am

Problem with retention time consistency June 18 - 08:33 am

UV-response March 26 - 09:47 pm

Correlation RT- physical properties April 9 - 06:25 am

Ion pair problems with TSK gel Super SW 3000 size exclusion columns? March 27 - 12:46 am

HPLC of quaternary amino group containing compounds April 24 - 09:11 am

Mobile phase degassing system February 27 - 02:47 pm

Shodex kc811 March 28 - 06:05 pm

BHT by SPE - Lot of applications available for BHT. Has anybody investigated... March 28 - 07:26 am

Plate Theory March 31 - 06:15 pm

Deivatisation of Sulfonic acid for HPLC/PDA April 10 - 02:12 am

Carboxylate anoins determination April 3 - 03:56 am

Analysis of Chromium VI Plating Solutions by Ion Chromatograhy April 7 - 06:08 pm

Glutathione April 4 - 06:44 am

Problem with impurities April 3 - 06:03 am

Does anyone have work with the Zorbax SB-phenyl column? April 16 - 02:11 pm

Albuterol-assay in drug product April 2 - 12:05 pm

GRADIENT QUESTION April 8 - 12:35 pm

Ascorbic acid in cosmetics April 10 - 03:56 am

Precipitation versus filtration for amino acid analysis of cell culture supernatants April 4 - 06:59 am

Separation of Triglycerides by LC April 24 - 06:09 pm

GPC Method for Tinuvin 322 April 6 - 11:56 pm

Agilent Diode Array - is it good? April 11 - 06:18 pm

Any methods for ketotifen fumarate? April 5 - 07:11 am

TLC spot reagents April 14 - 01:56 am

Set parameter in drylab software April 8 - 10:08 am

Guard cartridge/column for microbore columns? April 9 - 02:41 pm

Working with rat plasma April 10 - 11:54 pm

Dial-A-Mix April 11 - 02:19 pm

Separation of Proteins : April 17 - 12:40 am

Instrument vs. Human Mixing May 10 - 08:52 pm

Any idea for hplc separation for Nicotinic Acid, Pyridoxine hydrochloride and Papaverine hydroclhoride using a single method. May 8 - 10:44 am

GPC Method for Polyethylene/Polypropylene April 13 - 12:42 pm

Ion exclusion column April 14 - 10:44 am

USP Limits April 15 - 09:29 am

Liquid-phase microextraction April 15 - 08:26 am

Difference between parallel samples/results April 16 - 01:54 am

Accu Tag v/s Pico Tag April 16 - 05:23 am

PH 9 and Ion Pair May 8 - 03:00 am

Microdialysis and dopamine April 17 - 01:29 pm

Effect of internal diameter on column efficiency. April 23 - 06:51 pm

Light stabilizer April 22 - 11:19 pm

USP April 21 - 02:08 pm

Problem in peak purity May 23 - 10:27 am

Scalability of reverse phase HPLC method April 21 - 07:24 pm

Method Validation April 24 - 10:54 am

Surconcentration problem with organic amines April 28 - 01:04 pm

Standard sample shows extra peaks April 28 - 01:08 pm

Double peaks for thiophenol June 8 - 02:28 am

Preparative Scale Chromatography Suppliers April 22 - 10:21 am

Ion Pairing Chromatography April 28 - 03:34 pm

Molar extinction May 11 - 12:04 pm

Accuracy at detection limit April 29 - 10:39 pm

Data collection rate for Waters 2487 April 26 - 06:55 pm

Process scale LC contract labs April 25 - 10:38 am

Inter and Intra assay April 28 - 05:51 am

Effect of sample prep (composition) on retention time in GPC May 19 - 06:08 am

Tailing peak obtained by gel filtration May 2 - 12:05 am

HPLC Evaluation June 1 - 10:35 pm

Plasma sample:Protein precipitation by ACN---What exactly is precipited?? April 28 - 03:23 am

Solid phase extraction disk April 27 - 09:23 pm

HPLC method for analyzing Glycine May 9 - 10:31 am

GPC with dimethylformamide as eluent April 30 - 06:57 am

TBAH AND C18 April 30 - 05:44 pm

Normal phase, silica column, and water May 27 - 03:27 pm

LKB detector spares May 2 - 02:10 am

1,1,2-trichloro-trifluoroethane substitute? May 2 - 09:59 am

HPLC System Monitoring May 14 - 03:28 am

Simple CE application May 7 - 12:53 am

Vitamin A May 7 - 02:27 am

Preperative RP column recuperation May 8 - 03:12 pm

Peak quality during a gradient May 8 - 08:45 am

HPLC of peptides at high temperature! May 12 - 03:31 am

SEC mobile phase selection May 12 - 07:29 am

Chromatography Software June 3 - 04:18 pm

Supplier of IRMS May 9 - 09:57 pm

Agilent 1100 binary pump problem May 13 - 08:53 am

Use of Buffers in reverse phase systems May 16 - 03:25 pm

Lipopolysaccharides on HPLC May 12 - 07:17 am

Kanamycin Sulphate May 18 - 11:21 am

HPLC POLYAMINE ANALYSIS June 23 - 06:00 am

GLP May 13 - 12:52 pm

Different sample preps lead to different "Recoveries". May 27 - 12:57 pm

Protein present, but no 214nm trace May 19 - 09:35 am

Software Bug?? in Waters FractionLynx 4.0 May 14 - 05:33 pm

Parts May 15 - 05:21 am

ROBUSTNESS IN GRADIENT METHOD May 19 - 05:01 am

TESTING LINEARITY May 21 - 05:05 am

Porosity June 5 - 02:21 am

SEC - size exclusion chromatography of albumin May 19 - 08:16 am

Forced Degradation - Oxidation May 21 - 08:27 am

1,1-Diphenyl-4-piperidino-1-butanol hydrochloride ( difenidol hydrochoride May 20 - 03:07 pm

Picopure water?? May 21 - 02:09 am

Absorbance Ratios / Response Factors May 21 - 01:48 am

Detection Limit (% in areas) May 21 - 06:54 am

LOD and LOQ - help May 21 - 06:33 am

Mass Balance May 27 - 11:26 am

Response Factor / Absorbance Ratio May 23 - 12:37 am

Supplier of Naltrexone impurity reference standards May 23 - 12:43 pm

GRADIENT BLANK PEAKS REMOVAL May 31 - 09:24 pm

EQUIVALENT COLUMN TO SPHERISORB ODS May 28 - 07:52 am

Iodantipyrine May 25 - 01:04 am

High Speed HPLC May 28 - 12:10 pm

Assay average May 28 - 01:28 am

What mobile phase is required for Micelle chromatography May 31 - 12:08 pm

Stress testing May 27 - 01:33 pm

Biotage Parallex Flex 4 column May 12 - 11:41 am

Separation of carotenes May 28 - 05:28 pm

Retinoic Acid May 29 - 04:27 am

Gradient for smaller column? May 29 - 03:46 pm

Peak Area and Flow Rate May 30 - 12:17 am

How about the HPLCs developed by Cohesive? May 29 - 03:52 pm

MUX by Waters June 25 - 11:41 pm

HPLC Method. June 2 - 10:05 am

Plate counts of 500,000 per metre ? May 29 - 10:06 pm

Removal of placebo peaks for RS analysis ? May 31 - 12:31 pm

Antioxidant June 2 - 01:05 pm

Irgafos 168 oxidation product June 2 - 08:11 am

Spiking impurities ??? June 2 - 04:33 pm

Polyethylene glycol June 9 - 06:31 am

Loss of selectivity after only 50 Injections (RP) June 17 - 12:18 am

What column-brand to use for Related Substances N-Acetylcystein European Pharmacopoeia ? June 4 - 08:39 am

Looking for good HPLC procedure for Alendronate Sodium in Tablets June 3 - 02:35 am

Details about Ultrabase C18 June 3 - 03:01 am

Sodium Omadine June 6 - 06:34 am

Column Sample Loading June 4 - 07:00 am

Reversed phase vs. ion exchange--please advise June 6 - 12:15 pm

Chemical Structure June 10 - 05:12 am

Elimination of buffer after separation June 4 - 11:35 am

Chiral HPLC method June 10 - 05:19 pm

USP Pectin June 5 - 12:40 pm

Assay Recovery Difficulty June 10 - 08:41 am

Sensitiity June 18 - 04:44 am

Flavour peaks intereferance June 10 - 05:05 am

RP Amide Stationary Phases June 11 - 06:26 am

Solvent disposal June 17 - 02:29 pm

Simultaneous quantitation of Chlorpheniramine maleate, pseudoepdrine hcl and methscopolamine nitrate June 11 - 02:05 pm

What causes peak tailing and peak splitting in LC columns? June 14 - 02:09 pm

Can we transfer clarithromycin related substances method as per EP to ECD detection June 11 - 10:51 pm

Setting up an HPLC from scratch June 19 - 08:44 am

Column Classification June 12 - 10:51 am

Picolinic Acid analysis June 13 - 10:40 pm

Organic salts and bases June 17 - 12:08 am

PEEK June 16 - 12:40 pm

Impurities of drug products June 15 - 10:57 pm

Peak area changes while changing flow rate? June 14 - 08:49 am

Is TEAA also volatile? June 16 - 02:03 pm

Sepharose-arginine June 25 - 04:58 am

Please help with HPLC set-up!! June 19 - 02:02 am

Peak splitting probleam ? June 19 - 12:15 pm

HPLC peak separation question June 20 - 02:42 am

Condition for separating salt and ionic surfactant with reverse phase c18 column June 27 - 12:06 pm

Calculation help June 25 - 01:18 am

No HPLC pressure June 24 - 04:22 am

Forced oxidation June 27 - 01:58 pm

Purification of fatty acid June 26 - 03:26 pm

Opti-Lynx Thumbs Up or Down June 25 - 11:38 am

Monitoring vitamin concentrations June 27 - 06:14 am

Headspace (HS) GC: With or without split? June 20 - 08:32 am

Method for the dection of certain Azocolourants in Dyestuff April 25 - 05:22 am

Zorbax C8 4.6mmx25cm P.N. 880952.706 Agilent Technologies January 17 - 06:24 am

MOXIFLOXACIN AND GATIFLOXACIN CHROMATOGRAPHIC PURITY TEST BY HPLC March 10 - 12:28 am

Size exclusion column for small molecule April 5 - 12:59 pm

Removing sodium phosphate salt from folic acid derivatives April 15 - 04:17 am

Hi! separation of Root Exudate. May 25 - 10:35 am

Looking for an old paper January 15 - 10:05 am

Retrieve January 21 - 06:25 pm

Retention time of perylene?? January 23 - 06:06 pm

Do anyone of you? January 29 - 11:51 pm

DO YOU HAVE THIS ARTICLE February 17 - 01:45 am

Chemical properties in a WEB site ? February 10 - 03:13 am

UK supplier needed for PolyLC columns February 15 - 05:08 pm

High pH LC Separations April 7 - 08:08 am

Role of buffer and Ion pairing reagent in Reverse Phase Chromatography April 7 - 04:40 pm

Plate Count Anyone?? April 22 - 02:43 am

Flesh Chromatography May 22 - 01:03 am

Qualification of HPLC February 14 - 01:54 pm

Printing chromatograms April 17 - 09:42 am

Sample preparation for sodium benzoate November 21 - 11:56 am

Separating acetic acid and TFA by RP September 12 - 02:41 pm

Recovery Specification September 23 - 07:31 am

Linear instruments model 200 UV detector July 14 - 02:32 am

Agilent Software - ChemStation Plus or Cerity April 26 - 08:54 am

Vitamin B1 and Vitamine B6 May 14 - 11:07 am

LC method for corosolic acid June 14 - 10:23 am

Saw Palmetto by HPLC August 25 - 02:12 am

Column Info July 30 - 05:02 pm

Problem in peak purity September 26 - 03:38 am

Gentamicin analysis July 15 - 05:41 am

Margarine/fatyacids July 16 - 05:51 am

High temperature HILIC-column July 17 - 04:10 am

Help! Waters 2487 Display "Fatal error" June 15 - 05:06 am

Please help with my appalling maths! July 1 - 02:41 am

IC column July 4 - 06:51 am

Analysis of chromium with rp-HPLC using ion pair reagent. July 1 - 03:17 am

Peptides and protein affinity to metal July 3 - 11:15 pm

IC question July 1 - 08:43 am

Lactofen herbicide July 2 - 06:40 am

Maleic Anhydride!!!! July 6 - 12:05 pm

Scaling analytical methods up to semi-preparative July 4 - 03:03 am

Separation of Isotopic Compounds. July 3 - 11:29 pm

Agilent ChemStation Plus Help problem July 8 - 05:16 am

Mystery Peaks... please advise July 10 - 12:32 pm

HPLC Analytical Method to analyse Tacrolimus (FK506) June 18 - 05:01 pm

Linearity-Method Validation September 7 - 10:45 pm

How to separate D-ala-Dala dimer from its monomer D-Ala July 8 - 07:11 am

High pH July 9 - 07:39 am

Separation of Polyethylene Additives July 15 - 12:37 am

Regeneration of C18 Columns September 26 - 01:37 pm

Analytical Method Variability July 10 - 05:30 am

Inherited LC columns to test: Help!! July 19 - 05:03 pm

Recovery of Active from formulation at compression stage July 11 - 06:33 am

Drift in baseline using TFA acetonitrile July 11 - 01:25 am

Citrate bufer: urgent July 11 - 03:26 am

Internal Standard selection July 12 - 08:13 pm

Tolnaftate May 30 - 02:51 pm

HPLC-CLND Experience July 16 - 04:18 am

USFDA bioanalytical method validation guidelines July 16 - 05:23 am

RRF , RF , Correlation Factors and its dependance ? July 29 - 01:24 am

Low recovery in SEC-HPLC analysis of antibody October 24 - 12:38 pm

Moving-wire detector July 21 - 11:28 am

Why acually the salts like NaCl,KCl are used in mob.phase July 19 - 12:22 am

Poor Peak Shape August 1 - 12:58 am

Validation - acceptance criteria at the LOQ August 5 - 08:42 am

Zero intercept July 23 - 09:00 am

HILIC for rather non-polar compounds?? July 24 - 12:01 pm

Quantification of rider peaks July 22 - 04:26 am

Quality control July 22 - 06:09 am

RT decrease July 23 - 05:47 pm

Analysis of Mycotoxins and Aflatoxins by RP-HPLC -DAD July 23 - 05:37 am

NANO ALUMINA FILTERS FOR CHROMATOGRAPHY? July 23 - 12:11 pm

Peak Humps? June 6 - 07:46 pm

Piscidic acid July 23 - 09:45 pm

HPLC Samples from a Polymerization Rxn July 29 - 05:15 am

How to perform a specificity of a HPLC-RI method ? July 28 - 12:15 am

Refractive Index Detector Problems August 6 - 03:21 am

RP HPLC of geometric isomers August 4 - 05:38 pm

DIONEX ED50 for amino acid analysis May 22 - 05:54 am

Method for Tinuvin 622 August 4 - 07:00 am

Derivitisation June 10 - 12:08 am

Gatifloxacin and tailing probleam? August 11 - 12:59 am

GRADIENT PROBLEMS August 1 - 01:55 am

CLEANING OF COLUMN August 1 - 01:57 am

Protein separation September 16 - 03:06 pm

HPLC Vial Problem August 2 - 02:40 pm

Separation of small peptides August 2 - 02:35 pm

Ammonium acetate removal March 29 - 05:47 pm

IMPURITY QUANTIFICATION & VALIDATION IN COMBINED DOSAGE FORMS June 25 - 06:00 am

Automated organic chemistry with HPLC August 1 - 12:22 am

Multi�Electrode Array Detector August 1 - 05:56 am

On-line extraction August 5 - 04:37 am

Low volume mixer August 4 - 10:42 am

ChemStation Report Style August 6 - 02:54 pm

General questions on HILIC. May 22 - 07:37 am

PQ of LC system article written in Sept. edition of LCGC Mag August 7 - 01:13 pm

HILIC August 6 - 01:37 pm

Impurity quantitation August 21 - 03:23 pm

Mobile August 7 - 06:32 am

Phenoxy acetic acid analysis July 10 - 09:10 pm

Short guard column life August 10 - 09:26 am

Doxorubicin and liposomal doxorubicin August 12 - 04:21 am

Carboplatin related substance in formulation August 18 - 04:53 am

Retention in IEX August 14 - 08:35 am

HPLC of peptides at high temperature! August 11 - 01:52 pm

SPE cartridge vs. extraction disk August 12 - 06:34 am

Peak Purity Analysis August 19 - 11:58 pm

Suppressed IC analysis August 14 - 03:53 pm

Perfluorophenyl column: influence of flow rate August 18 - 04:06 pm

Replacement pump for Dionex 8200 series inline IC August 13 - 05:24 am

UV spectra and chemical structure August 28 - 02:42 am

HPLC-Method Validation after changing the temperature August 18 - 11:19 pm

Streptomycin in milk HPLC assay May 14 - 11:05 am

PEEK chemical compatibility August 15 - 03:48 am

Amide C16 Columns August 15 - 06:54 pm

Separation of org. acids/ketones/alcohols from apolar compound August 18 - 07:40 pm

Silica base and bonding chemistry August 23 - 01:09 pm

Find internal standard for stavudine and lamivudine August 21 - 06:49 pm

SPE can deproteinate bound form of drug in biological sample?? August 28 - 12:55 am

Negative peak for GPC column August 29 - 09:35 am

Column Killing Functional Groups August 27 - 05:37 am

SST - Regulatory methods August 22 - 01:32 am

Step gradients in RP HPLC August 29 - 09:58 am

Suspensions - mass/ml August 22 - 03:54 am

Mikro HPLC August 21 - 09:57 am

Determination of related substances in cyclosporins August 21 - 11:35 am

Diquat/Paraquat in Soil/Plant tissue August 26 - 11:51 am

Working with ion pair reagents August 27 - 10:46 pm

A working method fails to work later August 25 - 06:35 am

HPLC METHOD August 25 - 07:53 am

Peak splitting September 3 - 12:11 pm

Sample preparation for HPLC - how to improve recovery ? August 26 - 11:13 pm

Method Development August 26 - 04:53 am

System suitability requirements according to EP August 31 - 11:19 pm

Are peak capacity and retention factor the same? August 26 - 04:01 pm

RRT CALCULATION August 27 - 09:48 am

Stainless steel tubing and basic eluent September 3 - 10:30 am

THF VS Toluene as an Eluent August 29 - 08:30 am

Configuring HP1090 with manual injector under ChemStation v7 September 2 - 08:31 am

SEC assays and aggregation analysis September 5 - 02:37 pm

IEX September 3 - 12:22 pm

High pressure and low pressure mixer September 1 - 06:02 pm

Help! how to extract vitamins from multi-vitamin tablets? September 5 - 04:08 am

Bracketing of standards September 2 - 08:35 am

Naproxen sodium degradation October 18 - 05:30 pm

Glutaral USP September 2 - 12:26 am

Rising baseline in HILIC-separation September 2 - 05:19 am

Cyanocobalamin June 9 - 12:16 pm

Reproducibility in RT September 3 - 01:18 am

Polimeric Columns instead of ion-pair September 3 - 10:27 am

Gas removal September 4 - 01:01 pm

Area to noise threshold September 5 - 06:50 am

Cerity and Chemstation Plus September 5 - 08:35 am

Buffer Mixer problem? September 5 - 06:56 am

PBT oligomer standards September 4 - 10:09 pm

Tailing of halo carboxilic acids September 20 - 11:56 am

LC-UV of acamprosate calcium September 9 - 04:17 pm

Guard column selection Analytical scale September 11 - 02:52 pm

Saponins and HPLC with UV detection September 11 - 12:16 pm

Hplc-atrazine metabolites October 6 - 12:02 pm

Menthol by HPLC?? September 12 - 01:25 pm

Limit of Quantitation September 12 - 12:23 am

Agilent or Waters HPLC ? September 28 - 08:01 am

Dark, pigmented solutions in HPLC September 15 - 08:23 am

HPLC for room contaminants? September 16 - 06:30 am

Normal phase method development ? September 14 - 11:03 pm

Vendors of ( capillary ) monolithic column for small molecules? September 17 - 04:25 am

Peak shape probleam in normal phase LC? September 22 - 02:38 pm

Dual wavelength Vs change in wavelength September 17 - 10:13 am

Comparing fluorescence and UV detection September 26 - 12:42 pm

EDDHA Isomer separation May 14 - 06:39 am

Complex component September 19 - 07:29 pm

Analyzing mixture of very polar and very nonpolar substances. September 20 - 10:08 pm

Impurity / Related Substances for Ketoconazole October 20 - 11:53 pm

Calculate total impurity September 17 - 06:28 am

Need contract prep lab September 18 - 10:32 am

Searching for Rheodyne-compatible union October 1 - 03:18 pm

Taking care of narrow-bore HPLC column September 23 - 10:42 am

Protein analysis by hplc September 29 - 12:15 am

Help with SPE and supplements September 29 - 09:19 am

Carbon loading September 22 - 02:49 pm

Solubility of buffers in high organic media September 27 - 10:51 am

What do you look for in an HPLC? September 30 - 09:10 am

Expire Date for Mobile Phases September 29 - 12:41 pm

Ascorbic acid 2-phosphate magnesium salt by HPLC September 27 - 10:56 am

Need some advice on purchasing new HPLC system September 25 - 04:15 am

Problem with Benzo(a)pyrene in PAH analysis September 26 - 06:31 am

Column storage solvent. September 27 - 05:14 am

Mobile Phase Question September 27 - 06:59 pm

Liquid liquid extraction questions June 24 - 11:33 pm

High pressure problem with an anion exchange column March 28 - 07:41 am

Water HPLC grade quality September 27 - 08:31 pm

Peak Purity June 1 - 11:44 pm

Sample preparation for Stability Studies September 27 - 01:44 pm

Chromatogram Scaling September 27 - 02:04 pm

Bromopride Analysis September 27 - 02:15 pm

Linearity problems September 30 - 09:24 pm

Composite (pooling) samples for impurtiy analysis September 29 - 12:15 pm

Ambient temp. method for cyclosporine September 30 - 02:51 am

Corn oil suspensions; azacrown macrocyclic Mn complexes October 7 - 06:40 am

HPLC of intact sulfite adducts October 1 - 08:28 pm

XTerra RP Columns and Ion-Pairing October 6 - 09:32 pm

Urine Components October 1 - 11:41 pm

Brown/Black residue after evaporating HCl/methanol/acetonitrile October 1 - 08:20 pm

Support contracts and disaster recovery for CDS October 2 - 11:10 pm

How to differentiate acid form from its salt form October 6 - 10:53 am

Shifting retention times October 25 - 07:41 pm

Problems with Cloxacilline Sodium analisys October 2 - 10:43 am

Hplc October 6 - 03:25 am

Signal to Noise Ratio calculation June 13 - 08:04 pm

Method for Metadate (methylphenidate HCl) October 7 - 08:58 am

HPLC method for Methylphenidate hydrochloride October 13 - 05:16 am

HPLC separation of benzaldehyde and acetophenone October 26 - 09:02 am

HPLC for Surfactant analysis July 14 - 10:40 am

RP-Amide C16 October 10 - 03:33 am

Help! High back pressure October 15 - 04:55 am

Best Solvent for PIB October 9 - 11:33 am

Creatinine: a very sensitive method October 10 - 08:05 am

1.8um & 3um particle sizes October 18 - 10:01 am

Spike instead of internal standard October 17 - 08:40 am

Rp-Hplc seperation of vitamin D3 and vitamin E October 10 - 09:43 am

Similarities between electophroesis and chromatography October 10 - 04:53 pm

Applications Literature / Books on HPLC October 22 - 10:45 pm

BEHAVIOUR OF C-4 COLUMN AND ITS COMPARISION WITH OTHERS October 12 - 09:21 am

Sugar/Sugar alcohol analysis May 31 - 07:10 am

Sensitivity and residuals plot October 13 - 04:45 am

Testing solvents for HPLC use October 16 - 05:29 am

Waters HPLC error message October 21 - 12:25 am

Solvent Filters October 15 - 06:07 am

Correct Connections? October 21 - 12:00 am

Buffer Solubility October 17 - 12:11 am

HP1100 autosampler "failed injections" October 16 - 12:47 pm

Specification on resolution October 20 - 05:03 am

Baseline disturbances in IEC October 21 - 06:24 pm

Method qualification/validation? October 16 - 11:18 pm

Homogeneous powder sampling October 20 - 07:31 am

Capillary electrophoresis apparatus October 17 - 05:14 am

Analytical Method of Fosamine Ammonium October 17 - 07:34 am

Tailing Factor October 20 - 11:34 pm

Assay October 19 - 10:42 pm

Need help to select a ELSD June 25 - 02:28 pm

DryLab for undergraduate course October 21 - 08:46 am

Amide C16 Discovery & Bonus-RP October 25 - 07:34 pm

Unstable respons of OPA-derivatized lysine October 31 - 12:11 am

Help: TOPO analysis October 22 - 05:10 am

LC-Analysis of Hamamelis virginiana L. October 23 - 06:09 pm

Powdered milk October 27 - 10:51 pm

DS-Plus Auto Suppressor October 27 - 10:27 pm

Looking for resources for preparative chiral separation of amino acids October 28 - 09:52 am

Serice 2000 Pump Gradient Calibration Process October 24 - 06:47 am

Poor recovery of HMW species relative to monomer on SEC HPLC October 29 - 07:03 am

Alternative HPLC Column is Needed !! October 24 - 06:54 pm

Isn't HILIC just good old normal phase? October 28 - 06:31 am

TFA /TEA Ion Pair reagent October 29 - 06:17 pm

Sulfonic acids October 30 - 05:28 pm

Proteins that absorb at > 500nm ? October 29 - 11:43 pm

Irregular silica, 5um 2.1cm ID HPLC column??? October 30 - 06:42 am

Maillard adducts October 30 - 07:27 pm

Vacuum degasser hoses July 10 - 12:37 pm

Sulfonic acids October 29 - 06:42 am

IEX - transient pH shift March 9 - 03:01 pm

Unknown peak in HPLC analysis of Soduim Benzoate April 21 - 04:43 am

Refoling checking by RPHPLC May 20 - 12:23 am

Column dead time?? May 25 - 03:33 am

Unknown peaks July 21 - 06:40 am

DEAD VOLUME July 3 - 01:07 am

Problem with high potency results in analysis July 15 - 10:58 pm

Reproducibility probleam with lichrosphere RP8 July 15 - 12:24 am

Drylab August 14 - 01:58 pm

Is there functional group exchanging in the separation process August 5 - 04:38 pm

Capillary electrophoresis forum sites July 15 - 01:08 am

UREA October 10 - 02:49 am

Surveyor / Atlas reliability and functionality. October 28 - 05:08 am

Empower info September 10 - 07:30 am

Water-soluble vitamins in feed July 23 - 08:30 am

SFC Information? July 23 - 08:34 am

Thin-Layer Chromatography July 23 - 01:47 pm

Aflatoxins November 18 - 09:39 am

CIS TRANS SERATION April 23 - 07:54 am

Quick IC question January 7 - 02:04 pm

High Area Count on First Injection Only Using RI Detector January 14 - 10:44 am

System Suitability Requirement for FTIR Spectrometer January 16 - 02:51 pm

Accuracy for impurity method validation December 1 - 05:55 am

Dionex Summit HPLC System January 9 - 06:37 am

HILIC activation problem? December 18 - 07:04 am

Inject plasma directly onto a short C18 column April 7 - 05:15 pm

Analysis of codeine phosphate tablets September 30 - 08:49 pm

Mushroom beta glucans November 5 - 06:54 am

Analysis of liposomes November 12 - 03:43 am

Conversion December 2 - 08:48 am

2,4-DNP recipe November 14 - 01:28 pm

Empower Software December 4 - 08:51 am

L-DOPA by RP-ECD November 26 - 12:25 am

ChemStation sequence problem May 20 - 01:27 am

Ion exchange mobile phase November 3 - 01:51 am

Allantoin in Cosmetic Formulations. November 3 - 06:11 am

Chloroform as RP solvent? April 19 - 12:30 pm

Chromatography strip November 5 - 11:31 am

Gilson Aspec XLi Automated SPE May 18 - 11:12 am

Using Retention Time to determine column-death November 16 - 04:27 pm

Prep hplc and column splitter November 6 - 12:54 am

Impurities November 6 - 11:52 am

Retention of amidines, amides and diamines November 7 - 10:02 am

NMR calibration November 7 - 12:11 am

Anyone have experience of using a Beckman System Gold with another manufacturers Fluorescence Detector November 7 - 03:09 am

HPLC method for methanesulfonamide November 9 - 01:01 am

Analysis of Trace Silicone Oil November 13 - 09:04 am

Analysis of PAH's in light gas oil by HPLC April 28 - 06:48 am

Determinaton of divinorin November 8 - 07:36 am

Determination of psilocybin, psilocin, baeocystin November 7 - 11:47 am

Analysis of Pentaerythritol Tetrastearate (PETS) November 11 - 10:33 pm

Low recovery by PPT procedure November 17 - 12:09 am

EDC / EDU Analysis November 14 - 01:35 pm

HPLC maintenance course November 18 - 06:55 am

Porous vs Nonporous particles November 17 - 01:24 pm

Attached cdf files November 18 - 03:59 am

Deactivated ? endcapping? May 22 - 08:01 am

Validation of impurities method. HELP!! November 18 - 01:32 pm

System suitability tests limits May 26 - 02:27 am

MAU April 29 - 02:09 pm

Problems with Polysorbate 80 January 23 - 05:18 am

Phenacetyl Prednicarbate November 17 - 07:17 am

Mucoadhesive mouthwash containing diclofenac - Problem in sample preparation for impurities quantification!!! November 19 - 03:32 pm

Normal Phase HPLC solvents November 21 - 02:01 pm

New dead volume November 18 - 05:07 pm

Column November 19 - 07:34 pm

Column November 18 - 07:00 am

Pressure drop problem November 19 - 02:29 am

GABA analysis April 20 - 10:21 am

Ammonium formate buffer pH 4.5 August 10 - 10:52 am

Protein 'sticking' November 21 - 03:43 am

High recovery in Acuracy November 21 - 12:57 pm

Agilent Hardware and Waters Software March 31 - 11:22 pm

C18 vs. C18 AB November 20 - 03:05 am

Selfpacking of prepcolumns (stability) November 24 - 12:23 pm

Methods validation by HPCL November 20 - 08:34 am

HETP calculation? November 25 - 09:03 am

Wierd peak waters hplc May 20 - 10:23 am

Peak identification in solvent front November 26 - 06:08 am

Assay + related compounds in same chromatographic run November 26 - 12:56 am

PKa? May 20 - 06:26 pm

Analyitcal Lab. November 26 - 08:04 pm

Book recommendation for HPLC December 2 - 01:31 pm

Agilent 1100 System Fluorescence Detector November 28 - 04:07 pm

Ammonium sulfate November 29 - 06:59 am

Gradient runs ? gradient method developement ? linear gradients curve 6? May 24 - 04:10 am

Free amine and its salt--How to distinguish them December 1 - 05:46 pm

Is all detector are same ? December 16 - 05:50 am

Organomercury HPLC-APCI problems December 2 - 07:58 am

Optimize seperation op bezoic acids April 16 - 11:08 pm

How can I report tailing factor December 17 - 04:24 am

Role a standard/sample diluent May 15 - 09:43 am

Non-linear detector response December 3 - 03:05 am

Mycotoxins in animal feeds December 9 - 04:04 am

Sample precipitation in gradient December 8 - 12:59 am

PENTAERYTHRITOL TETRASTEARATE Analyis help December 4 - 09:30 am

Analysis of Chimassorb 944, Cyasorb 3346 and Chimassorb 119 December 4 - 09:13 pm

Food colours analysis December 9 - 01:47 am

Sample carryover December 8 - 02:53 am

How to qualify the HPLC Column ? December 11 - 08:42 am

Determination of sodium salicylate December 8 - 01:23 pm

Recovery Calculation January 7 - 05:18 pm

Literature December 12 - 09:57 pm

ELSD detector May 10 - 05:20 am

Anion and cation exchange columns for protein separation December 10 - 05:01 pm

Compatibility of hepta fluorobutyric acid December 10 - 08:55 am

HPLC December 11 - 03:18 pm

Buprenorphin December 11 - 08:02 am

Parameter for sepration January 7 - 05:09 pm

Impurities isolation at small scales December 12 - 11:00 am

Direct Plazma Injection June 16 - 08:53 pm

Measuring system volumes ? December 15 - 11:02 am

Exciting Job Opportunities December 16 - 05:55 am

USP Buffer Preparation December 18 - 04:11 pm

Delayed injection December 17 - 03:34 pm

Equilibration of ligand-exchange column December 16 - 04:53 am

Extended uv lamp hours beyond 2000 December 18 - 02:11 pm

Injection Volume December 18 - 10:28 am

Have a same RRF on different UV-VIS detectors? May 14 - 09:35 pm

Consumer Reports for Analytical Instruments January 6 - 02:44 pm

Buffers Concentration December 23 - 12:14 am

Detection of sucralose - comparison of columns December 18 - 02:56 am

Shimadzu SIL-10ADVP Z-axis offset problem? January 3 - 05:03 am

UV at low wavelength December 19 - 04:38 pm

Chiral separation December 22 - 08:08 am

Problem: linearity variation of instrument December 26 - 03:17 am

Enatiomeric Seperation January 2 - 01:23 pm

Sample prep advice January 14 - 08:53 am

Optimum HPLC flow rates December 30 - 03:16 pm

Free Homocystein December 30 - 12:20 pm

Something non-HPLC December 31 - 06:51 am

IC instead of AA January 7 - 08:12 am

Asda January 27 - 07:32 am

Equilibration time January 8 - 02:14 am

Sucralose in soft drinks January 5 - 08:01 am

INTERNAL STANDARD METHOD VALIDATION January 6 - 10:42 pm

Strange HPLC Trace January 12 - 01:31 am

Need Help - Waters LCM1 - Need suggestions on how to get rid of... January 8 - 11:06 am

Column restoration January 22 - 07:24 am

Descending drift ri baseline January 11 - 11:55 pm

Product contamination with stationary phase in prep. HPLC. January 13 - 08:14 am

Monoclonal antibody separation by HIC January 9 - 12:51 pm

Help! Increasing Peak Height! January 13 - 12:28 pm

Research Paper Follow-up January 11 - 04:41 pm

Non-stability indicating HPLC methods May 8 - 07:41 am

High Speed LC January 13 - 03:13 pm

Gradient Ion-Pairing Separation January 17 - 08:33 am

Methyl glucose January 14 - 10:47 pm

Needle alignment for gilson 231xl January 13 - 08:26 am

Halide salts and stainless steel HPLC systems January 19 - 04:16 am

Static UV Drift January 13 - 08:14 am

Analysis of cetylpyridinium chloride. Need Method!! January 19 - 05:50 pm

Unknown crop chemical January 21 - 01:31 pm

ELSD detectors in Pharmaceuticals analysis January 18 - 06:45 am

Need to revalidate the analytical methods? January 20 - 11:45 pm

Silica particle question January 21 - 12:41 pm

Method validtion of related compounds January 19 - 11:30 am

Zorbax SB-Aq January 23 - 05:59 am

Octapole amplifier output out of range January 20 - 01:54 am

Digital flowmeter for HPLC May 11 - 09:25 pm

HPLC of peptides at high temperature! January 27 - 06:08 am

"HPLC of peptides at high temperature" should be "Dilution effect on PA!-SORRY January 20 - 10:28 am

Linearity : Criteria for acceptance of intercept? February 5 - 02:18 pm

Molecular weigth distribution Dextrans USP/EP - What column(s) to use? January 21 - 04:12 pm

% RSD Error February 2 - 11:52 am

Bile acids-sterols/faeces February 17 - 05:48 am

HPSEC calibration??? February 11 - 08:55 am

Ion HPLC analysis February 3 - 02:03 pm

Benzo (a) pyrene probelms February 2 - 03:30 am

Vitamin D3 March 17 - 01:23 pm

Dye having a flat absorption maxima at 450 nm January 30 - 08:08 am

GPC calculation March 29 - 09:29 am

Reference wavelength January 28 - 11:12 am

Determination of TRIS? February 2 - 01:28 pm

Analyze GC results February 2 - 09:33 am

3 Q? : Autosampler & sample carryover & fluorescence monitoring February 3 - 06:10 am

Retention factor or capacity factor? February 4 - 08:06 am

QS February 4 - 02:10 pm

Stability Studies of Pharmaceutical Formulations February 3 - 11:11 am

Carryover issues March 30 - 08:46 pm

Ruggedness February 4 - 05:16 pm

Failed proficiency test -- help!! February 6 - 10:41 am

Mv --> RIU equation February 13 - 12:48 pm

Tailing factor - ion pairing - TFA February 16 - 02:10 am

HPLC grade water May 6 - 11:59 pm

Quaternary Amines on a Reverse Phase Column February 12 - 03:31 pm

Retention time change with reverse phase HPLC using buffer m.p. February 10 - 11:47 pm

Cyano columns February 8 - 09:53 pm

Ecd method February 12 - 04:15 pm

Linearity December 4 - 01:48 am

Fatty acids derivatization December 30 - 11:17 am

Unit of signal axis for UV chromatogram February 17 - 07:05 am

Peak shape change in HPLC March 17 - 02:21 am

Procedure for cleaning of UV detector cell February 16 - 08:27 am

Determination of pKa February 11 - 02:06 pm

Deconvolution February 12 - 03:16 am

Auto Zero (ECD) February 12 - 05:22 am

Shift of baseline March 30 - 01:34 pm

Veterinary premix February 23 - 01:30 pm

Conductivity detector February 12 - 05:57 pm

System Suitability Requirements March 11 - 01:23 am

Computer Validation in Laboratories February 11 - 11:25 pm

EP methods???? March 17 - 10:58 pm

Is plate number meaningful for gradient? February 14 - 04:22 pm

Mass Spec compatability with antioxidants February 15 - 03:12 am

Formic Acid as Mobile Phase February 15 - 11:49 pm

Loss of sensitivity February 14 - 10:09 am

Analysis of sparteine (alkaloid) in plants February 13 - 12:08 pm

Chlorophacinone February 13 - 05:44 pm

Hplc condition for phenolic acids separation February 16 - 01:51 am

Fluorescence detection February 27 - 12:06 am

Polar aromatics from creams February 22 - 10:37 am

Acetylcholine by ECD February 26 - 08:53 am

Aspartame Assay Method February 21 - 04:23 pm

How to calculate signal to noise ratio ? February 23 - 06:23 pm

"Ghost peaks"- is it our ammonium acetate buffer? February 19 - 12:07 am

Guanosine--Really Strange Stability Data February 19 - 07:20 am

Bed volume vs. injection volume? February 24 - 04:53 am

Restek Replacement Parts February 24 - 06:22 am

Peak area increase March 2 - 02:48 pm

Interference in blank while doing lc/ms/ms analysis of glimeperide May 6 - 07:34 pm

Some questions about HPLC February 22 - 11:43 pm

Long equilibration time for 2.0 mm ID columns February 24 - 12:23 pm

Use of Na2SO4 as additif in mobile phase February 27 - 12:03 pm

Waters 2795 pump part February 23 - 02:09 pm

Aromatic amines in methanol March 3 - 01:38 am

Base line problem February 26 - 07:03 am

Two peaks from pure enantiomer? February 27 - 01:24 am

Service contract - waters March 1 - 10:31 am

IEC - volatile mobily phase February 27 - 03:51 pm

Does pump feel tired? March 1 - 03:41 am

Are there any other chromatography forums? March 1 - 12:39 pm

11mm Electronic Vial Decapper February 27 - 02:52 am

Polymer packing hplc column March 2 - 06:07 am

Chromatography Mgmt. position in NJ February 27 - 10:45 am

Polydispersity March 2 - 09:15 am

Mass corresponding to m/z +83 March 2 - 01:44 pm

Non-linear calibration curve: a flipped quadratic model? March 1 - 06:30 am

Ion Exchange problem? March 3 - 11:22 am

Preparative HPLC for isolation of Compounds from Plant Extracts March 3 - 03:50 pm

Fluctuating Baseline March 23 - 08:39 am

Analysis of porphyrin on ACE column March 4 - 01:05 am

Protein concentration March 5 - 05:15 am

Analysis of epoxy resins March 5 - 09:04 am

Change in Retention time March 8 - 03:31 am

HPLC IMPURITY METHOD VALIDATION March 11 - 10:27 pm

Methods for Clenbuterol and Ambroxol March 5 - 09:41 am

Need a Borwin version running on NT, 2000 or XP... :-/ April 6 - 11:58 am

Sample preparation--96 well-plates or online plasma injection March 8 - 03:10 pm

Scaling HPLC flow rates for different column dimensions March 9 - 11:38 am

Iex transient pH drop March 9 - 07:53 pm

Integrating a tailing peak April 20 - 01:21 pm

APCI vs ESI March 18 - 08:15 am

Macroscopic pKa March 10 - 07:00 am

Molar vs v/v concentrations March 10 - 07:32 am

Gradient HPLC fluorescence detector March 11 - 12:00 am

Ion suppression mode March 12 - 09:28 am

Mechanism of ion suppression March 16 - 01:21 pm

HPLC and catecholamine assay March 12 - 07:08 pm

Sources containing data on stability of protein in different solvents March 17 - 02:03 am

Reproducibility with JASCO As-1559 Autosampler March 12 - 08:33 am

Propinox clorhidrate March 11 - 07:53 am

CHI Index calculation March 12 - 07:24 pm

Solubility test by HPLC March 12 - 05:38 am

Galacturonic, Glucuronic, Mannuronic Acid March 18 - 08:02 am

Bioequivalence study format. March 13 - 10:59 pm

Solvent peak March 16 - 01:17 pm

Recovery/Accuracy March 14 - 12:38 pm

Liquid/liquid extraction of lisinopril from human plasma March 22 - 09:11 pm

McGowan characteristic volume March 15 - 08:30 pm

POCl3 Analysis March 18 - 04:49 am

Flowmeter failure rate March 17 - 11:02 am

Decreasing gradient baseline March 18 - 10:36 am

Can't set the UV detector zero March 30 - 02:21 pm

Linearity March 19 - 08:23 am

Tandem mass spectrometry March 18 - 10:51 pm

Sample preparation in pharma quality control April 5 - 08:28 am

Help: The analytic methed of 1,3-propanesultone March 20 - 08:55 am

Polarity of Acetonitrile and Methanol March 22 - 03:26 pm

About recovery? March 24 - 05:39 am

Mobile Phase Question March 27 - 10:44 pm

System peaks in gradient--glassware related? March 27 - 10:52 pm

Germall - colorimetry vs HPLC May 7 - 11:05 am

Can residual silanol groups in a C18 Sep Pak Vac act as a cation exchanger? March 23 - 08:20 am

Two opposite ideas about surfactant on HPLC March 23 - 08:16 am

Column Overload March 24 - 02:13 pm

Solution Concentration March 23 - 04:53 am

Microgram/microliter solution March 23 - 04:46 am

Determine ppb level Fluoride in ppm level sulphate solution April 2 - 03:28 pm

A Problem with FMOC derivatization. March 26 - 12:35 am

Piston pump for 30 psi 0-20ml/min March 24 - 10:14 am

Vitamin C HPLC method March 31 - 06:08 am

Chromatogram March 26 - 04:17 am

HPLC - student needing help February 21 - 04:25 am

A Problem with FMOC derivatization. May 17 - 12:56 am

Bethanechol chloride hplc method March 24 - 02:32 pm

Determination of vitamin B6 in dairy product March 24 - 10:50 pm

Buffers and Chromatography March 25 - 03:42 pm

Low level anion analysis March 24 - 02:25 pm

Conditions for forced degradation March 25 - 04:02 am

Negative RI Peak March 25 - 04:11 pm

HPLC assay for loperamide, ivermectin, or cyclosporin A March 25 - 07:52 am

Using ethanol in RP-HPLC May 6 - 08:55 am

Do you have any experience for the analysis of ceftiofur metabolites in milk sample? March 22 - 07:16 pm

Operational and Performance Qualification April 27 - 01:28 pm

HPLC METHOD FOR TACROLIMUS May 25 - 04:45 am

Innowax Column March 25 - 12:08 pm

Serious tailing when using column packed with porous silica gel to which benzenesulphonic acid groups March 22 - 08:25 am

POOLED DISSOLUTION March 22 - 04:11 pm

Analysis of (dex)panthenol by LC March 24 - 12:59 am

Sunscreen Method Validation April 7 - 02:25 pm

Relationship of response factors to molecular weight March 30 - 09:58 am

Silica March 27 - 03:15 pm

HPLC baseline drift April 5 - 02:19 am

Help April 1 - 12:52 am

Retention Index April 2 - 10:50 am

Preparative Columns Packing Proceedure April 10 - 04:35 pm

Desactivating normal phase columns? March 30 - 03:25 pm

HPLC Column Conditioning March 30 - 06:55 am

Replicates vs precision in validation April 23 - 11:50 am

Triethylamine April 8 - 12:59 am

SEC column was gone after storing in 25%ACN? March 30 - 03:31 pm

HPLC theorie - question? March 30 - 12:22 pm

When to do a method transfer March 30 - 10:39 pm

Misfolded protein March 31 - 04:42 pm

Separation of 2-AB labeled Glycans - Column selection March 30 - 09:19 am

Waters W2996 PDA flatline April 4 - 12:16 pm

Pullulan Standard Supplier? April 23 - 01:54 pm

Reference for Spectroscopic Detection in HPLC March 31 - 03:45 pm

Chiral Derivatization for the Drug Bupivacaine April 2 - 09:10 am

Origin of 'water peak' in different detectors? April 3 - 07:06 pm

Organic acid in natural compost April 2 - 01:33 am

Ion exchange troubleshooting April 5 - 08:13 pm

Flow rate greater than setpoint with agilent 1100 pump April 5 - 09:24 am

Solid phase extraction (SPE) procedure for cleanup of serum April 1 - 03:37 pm

Ghost Peaks April 2 - 03:52 pm

Prep Users April 2 - 08:01 am

C-18 column April 30 - 12:33 pm

C-18 column April 18 - 10:21 pm

ChromQuest Software Question May 24 - 04:23 pm

Prep TLC Fluoresent Indicator Extractable? April 3 - 09:18 am

Open-tubular HPLC April 13 - 04:56 pm

IC method April 16 - 09:29 am

Soil polysacharide analysis April 8 - 10:58 am

HPLC of peptides at high temperature! April 5 - 02:25 pm

Sorry for the wrong title! !CEC-HPLC or HP-CEC? April 5 - 09:01 am

Restriction of PDA detector usage based on USP general chapters-HPLC-Detectors April 19 - 08:40 am

Visualization of Lactones in thin layer chromatography April 8 - 04:49 am

Linearity Problem on API 3000 April 7 - 06:52 am

I need a good method for Carbamates on Oranges April 6 - 04:07 pm

Analysis of Acrylamide with HPLC April 9 - 04:20 pm

Buffer preparation April 21 - 07:55 am

Detect dimethyl succinate April 12 - 10:42 am

Does only damper damage may explain increased pulsation? April 12 - 08:09 am

Pork amino acid analysis April 19 - 11:38 am

Impurities analysis of DMPG April 12 - 09:17 am

Separation of UDP-GlcNAc and UDP-GaINAc April 12 - 12:11 pm

Which one?????? April 15 - 06:59 pm

Random Drifting Baseline April 20 - 06:37 am

5N HCl Evaporation April 13 - 11:47 pm

Waters Millenium 32 SW Wanted May 5 - 02:13 am

Stability May 6 - 05:02 am

APG(Alkyl Polyglocoside) and cellulose April 16 - 03:17 pm

Loss of prime using n-hexane MP April 16 - 02:17 pm

PAH's ELUTION ORDER AND I.D. April 15 - 08:16 am

High peak area in 1st injection of 5 replicates May 19 - 10:42 am

Positive or negative ion for Valproic acid? ES or HN? April 16 - 10:45 am

I have a drift in the blank!!!!!!-help April 18 - 10:30 pm

TNBP and Triton detection issue May 24 - 06:38 am

Any one know Waters ZQ2000 well, Pls! April 19 - 05:22 am

Is it possible? May 4 - 11:22 am

Quantitation of Unknown Impurities with UV Detection April 20 - 06:46 am

Vitamin 25-OH-D3 with Electrochemical Detection April 18 - 05:20 pm

Vitamin B1 and B6 in blood April 18 - 05:18 pm

Azide by RP HPLC April 20 - 04:51 pm

TFA in mobile phase April 20 - 12:27 pm

HPLC Millenium software problems_Waters May 19 - 02:41 am

PH/pKas April 23 - 07:39 pm

Analysis of 3-aminopyrazole-4-carbonitrile April 25 - 11:10 am

THF as mobile phase modifier in RP HPLC April 26 - 07:05 am

Error Messages on an Alliance System April 23 - 02:49 pm

Unknown peak in HPLC analysis of Sodium Benzoate-NF April 23 - 01:36 pm

Mobile phase for isolation of UTP by DNAPac PA100 column April 30 - 09:17 am

Methyl malonic acid April 21 - 04:37 pm

TLC for oligosaccharides? April 21 - 09:19 pm

HPLC NORMALISED METHOD CERTIFICATION April 22 - 01:16 am

ID tests for flavor April 22 - 02:03 pm

Diode Array Detector vs. Photodiode Array Detector April 23 - 08:18 am

Ion pair LC of amino acids April 23 - 06:55 am

Separation of alpha and beta carotene April 28 - 04:56 pm

DICLOFENAC SODIUM AND MISOPROSTOL HPLC METHOD April 27 - 04:37 pm

Acetaminophen solubility May 24 - 05:32 am

Lifetime of Ag/AgCl Reference Electrode April 25 - 09:46 am

Determination of thiamine in serum April 25 - 03:06 am

Removal of salts like tris April 29 - 04:11 am

Scratches on working electrode April 29 - 04:28 am

Solvent front negative dip, how to minimise? May 3 - 06:03 pm

Impurity analytical method development for organic acid/basic salt April 28 - 08:20 pm

Sampling amounts vitamins w/hplc May 3 - 08:38 am

Dynamic range of Agilent HP1100 May 8 - 05:53 am

Shimadzu or Merck May 19 - 11:43 am

Elektrochemical Detection Questions April 30 - 06:48 pm

Post-column addition May 2 - 02:39 pm

Alkaloid isolation by ion exchange chromatography April 6 - 05:55 am

Acidic compounds HPLC analysis April 27 - 06:02 am

Purge Valve Question April 29 - 08:41 am

Single point calibrations + accuracy May 5 - 01:21 am

Agilent 1100 system May 8 - 05:46 am

Pka of substances and pH of mobile phases May 3 - 10:06 am

Carbocysteine May 2 - 02:48 pm

Quantification of a sum of components May 4 - 08:20 am

Ion Chromatography-Indirect detection May 3 - 09:35 am

Conc. Acids May 3 - 07:55 pm

Pimetixen?????????????????????? May 3 - 01:17 pm

About THF as a mobile phase May 11 - 02:13 am

EDTA as modifier causes problems in RP-LC? May 7 - 05:25 pm

Suggestions for Needle wash solution May 4 - 07:42 pm

Peak spliting and Temp May 4 - 04:40 pm

%RH specs in an LC lab May 4 - 06:36 am

Forgive my ignorance! May 5 - 10:19 am

Need help for LC/UV dextromethorphan HBr determination May 5 - 05:09 am

Ammonium Hydroxide as a High pH buffer May 14 - 06:40 pm

DCM/MeOH/H2O as dissolution solvent May 8 - 08:39 pm

Why when I use gradient I cannot consider the number of theorical plates? May 14 - 09:00 pm

Sunscreen analysis May 10 - 04:20 pm

Determination of Dextromethorphan Hbr using HPLC with UV detector April 30 - 05:32 am

Analyses of DMPG (1,2-Dimyristoyl-sn-Glycerol-3-(Phospho-rac-(1-Glycerol)) May 6 - 07:49 pm

Extraction for nicotine May 13 - 12:11 am

MRLs sulfonamide in penaeus monodon May 6 - 08:26 pm

Response factor May 7 - 03:28 pm

Benzalconeo Chloride assay May 24 - 09:49 am

Oligonucleotides in column May 13 - 01:18 am

How to analyze EDTA? May 14 - 02:36 pm

Shimadzu manuals May 19 - 11:18 am

Analyst 1.4 - Error message May 10 - 08:49 am

LC May 10 - 08:38 pm

Hplc method development for detecting ascorbic acid and dehydroascorbic acid May 17 - 06:24 am

Digoxin May 12 - 12:01 am

Baseline drift conundrum! May 19 - 11:04 am

How to do transport Alignment for auto-sampler HP1100? May 13 - 06:29 am

Seat Capillary of HP1100? May 11 - 09:31 am

Injection problem! May 13 - 06:26 am

Validation of assay for tablets May 13 - 05:53 pm

Sodium 2,2-methylenebis-(4,6-di-tert-butyl phenyl) phosphate solution May 12 - 02:40 am

Homework and Test Answers May 13 - 01:12 pm

Air bubbles and low pressure after degassing May 14 - 07:44 pm

Separation of amino butyric acids May 18 - 05:43 pm

N-pi and pi-pi interactions in HPLC May 20 - 06:12 am

Broad peaks (HPLC) May 19 - 10:51 am

Anhydride & furoyl acid application May 14 - 09:51 am

Silica 60 and Silica 100 Columns May 13 - 08:41 am

HPLC Cesium Formate Solution, Sulfate detection, possible interferences May 18 - 06:06 am

DMSO as a sample solvent May 18 - 03:10 pm

Cyanide in wine May 21 - 06:41 am

HPLC Training - How do when an analyst is trained May 18 - 05:09 am

High Metal H2SO4 Organic Analysis May 14 - 02:22 pm

Needle voltage in electrospray May 16 - 11:44 am

Analysis of Steroids - Anabolic May 18 - 11:42 am

What is chromatography? May 17 - 07:52 am

HPLC method May 18 - 06:19 pm

One question about Agilent 1100 Standard Autosampler May 18 - 10:40 am

A interval of 74! May 21 - 07:34 am

Analytical Method Validation for Natural Products May 18 - 11:57 pm

HPLC grade water and solvents, Best? May 18 - 06:01 pm

Creams and shampoos May 25 - 05:19 pm

C18 compatible mobile phases May 25 - 06:08 am

Methoxy phosphonic acid sodium salt May 23 - 08:20 pm

Course in method development May 19 - 09:23 am

Peak Tailing and Fronting May 21 - 11:10 am

About DAD May 26 - 02:41 pm

UV Method for Metformin Tablet May 20 - 04:03 am

Gradient runs in micellar and ion-pair RP HPLC May 23 - 06:14 am

Polymer Labs Evap. Light Scattering Detector - high pressure drop May 20 - 06:51 am

Chemstation - Relativ retentiontime May 20 - 11:33 am

Cell Sieves May 21 - 12:17 am

TFA and column care May 24 - 10:34 am

Sodium Floride quantitation using HPLC with Pulse electrochemical detector May 26 - 05:57 pm

Methimazol TLC or HPLC May 24 - 04:22 am

Lower limits of detection of acid small compounds with 4.6mm columns May 25 - 09:41 am

Agilent Chemstation: grouping May 26 - 03:02 pm

Question about column for nucleotide separation May 25 - 08:26 pm

Microbiological Method Validation May 25 - 02:29 pm

SPE Society May 25 - 05:48 pm

How to develop an HPLC method for a protein stain? May 26 - 03:31 pm

Limit of trifluoroacetic acid in normal liquid May 27 - 01:42 am

Benzyl Benzoate soap May 27 - 09:44 am

What is the suitable mobile phase in paper chromatography for metal cation analysis? May 26 - 10:39 pm

Digoxin May 29 - 11:06 am

Agilent pump needed. May 28 - 11:55 am

HPLC - EDTA in pharmaceutical Preparations May 31 - 02:50 pm

Thiamine HCL May 30 - 04:42 pm

Beginner's question August 29 - 02:09 pm

Resolution of enantiomeric pair August 27 - 10:36 am

Strong negative deflection August 27 - 12:47 am

Dilution of protein precipitates August 27 - 02:45 pm

Acid chlorides August 27 - 03:47 am

TFA at 215nm August 27 - 08:38 am

LC Method for Diosgenin August 26 - 03:02 pm

LOD for scopolamine August 26 - 09:02 pm

Integration in HPLC August 27 - 01:05 pm

Good practice for HPLC - preparing dilution of analyte August 26 - 11:49 pm

Purity methods vs Content methods August 26 - 07:29 pm

HPLC Iso-Elutorphic strenght for IPA August 25 - 11:29 pm

Statistics Software August 25 - 08:54 am

HILIC/ELSD August 26 - 02:05 am

Effect of flow rate on separation August 28 - 06:35 pm

GPC/SEC August 27 - 08:34 am

HELP !! -shikimate detection August 27 - 08:55 am

Quaternary ammonium chlorides August 26 - 06:27 pm

Peak Tailing August 27 - 09:25 am

Undulating baseline August 24 - 11:23 pm

Wavelength accuracy August 26 - 12:16 pm

Peak in the blank August 23 - 04:18 am

How to clean the autosampler! August 28 - 02:13 pm

What is "Dead volume"? August 25 - 10:05 pm

Value of Slope/Threshold August 22 - 06:52 pm

Euphorbias and TLC August 22 - 08:22 am

Validation method for different strengths of drug August 26 - 12:05 am

Assay of folic acid August 21 - 07:57 am

Metformin and its cyanoguanidine impurity August 22 - 03:59 am

Assay method for glibenclamide/metformin August 21 - 07:28 am

THF in mobile phase August 24 - 10:44 am

Estimation of Retention time Analytical -> Preparative August 20 - 03:17 pm

Peak in blank reagent after derivatization! August 26 - 08:44 am

Switch valve between columns August 19 - 12:53 pm

A general question about calibration curve August 22 - 05:48 pm

HPLC Glossary August 19 - 10:55 am

Unsure with terms glossary August 20 - 10:09 am

5 Peaks in DNPH blank. August 20 - 09:06 am

Polarity index August 23 - 12:05 pm

How does pH affect the elution time of your analyte peak August 21 - 08:24 am

IPA flushing August 20 - 05:51 am

PDA detectors August 18 - 03:45 pm

Unsure with some terms... August 19 - 10:14 am

Procedure to wash a dirty system! August 18 - 11:12 am

PMT Gain in fluorescent detector. August 18 - 11:14 am

Scopolamine August 18 - 02:16 pm

General conductivity value for ions August 18 - 07:32 am

Confuse with the calculations for concentration of stock August 18 - 08:53 am

Pressure problem with RP_HPLC August 25 - 05:21 am

Accuracy August 17 - 02:47 pm

Alltech ELSD 2000ES compatible with Waters Alliance System? August 18 - 10:07 am

System Suitability HPLC August 20 - 12:18 am

ELSD for Polymers? August 19 - 05:35 am

LC AnalysisToluene sulfonic acid and anhydride August 17 - 07:14 pm

Inkjet analysis by HPLC August 17 - 03:06 am

Analytical method development without impurities August 27 - 04:20 am

A constant deep before my peak comes out August 25 - 07:44 pm

Thermo Finnigan Surveyor LC System? August 26 - 02:45 am

Lidocaine August 16 - 06:59 pm

Bio-HPLC training course August 19 - 12:59 pm

Beginner�s question August 16 - 12:29 pm

Retention time shortening in ion-pairing chromatography August 17 - 06:28 am

Dead volume in possible excess tubing August 16 - 08:57 am

DAD vs. DWD S/N-difference. August 13 - 04:49 am

Revalidation of a method August 17 - 02:01 am

Compatibility of Bio-Beads SX-3 with Solvent August 12 - 08:31 pm

HPLC Maintenance August 19 - 09:01 am

ChemStation - Run Macro when Seqence started August 16 - 12:35 am

Stability of acids in methanol August 15 - 07:21 pm

Repeatability of injection August 12 - 05:41 am

Hitachi HPLC injector carryover August 25 - 10:37 am

Method Validation - RSD% August 13 - 12:49 am

GLP Labeling August 11 - 12:51 pm

I really need help to eliminate my ghost peak! August 18 - 03:43 am

A quick and dirty method for analysis of polyethyleneimine August 21 - 05:27 pm

Column Capacity study August 21 - 05:35 pm

Sodium lauryl sulfate is bound to damage a CN column? August 18 - 04:01 pm

Misoprostol free acid-stability problem August 10 - 05:22 am

What's a surrogate ? August 17 - 11:24 am

Newsgrouserver / List August 10 - 05:01 am

Sodium azide storage time August 21 - 05:41 pm

Wild EDTA in Syrup August 21 - 05:50 pm

Why peak areas are decreased over time??? August 9 - 09:09 am

Ammonia removal in amino acid buffers August 18 - 02:08 am

What means T.DBL? August 13 - 01:03 am

Carbohydrate Detection Limits August 21 - 05:58 pm

Run time for HPLC quantification of the impurities in drug products August 23 - 09:17 am

Wavelength switching vs. dual wavelength collection- GMP August 6 - 12:29 pm

Angstroms to mesh? August 7 - 06:01 pm

Linearity test using one solution different injection volume August 26 - 11:30 pm

Peptide Seperation with Perchlorate Mobile Phase August 18 - 01:08 am

HPLC - Cremophor and paclitaxel August 9 - 04:55 am

Analysis of 8 "essential sugars" August 18 - 05:26 am

Preconcentrator for liquids August 5 - 08:03 am

Pramipexole chiral method August 5 - 03:03 am

RSD, %RSD, CV, %CV August 5 - 08:46 am

Derivatization of Topiramate (HPLC) August 4 - 01:06 pm

Relative respose factor August 6 - 12:17 pm

Method validation pack for Chemstation plus August 4 - 01:40 am

Tetrabutylammonium cleanup August 15 - 08:14 pm

LC Column with usp L57 packing August 4 - 09:23 am

Cysteine and Cystine August 10 - 02:57 pm

Loss of % CV between two std weights within a sequence of injections August 11 - 04:45 am

Detection wacelength August 3 - 10:22 pm

GMP in drugs August 12 - 01:00 pm

Hexaleucine August 4 - 02:17 am

Determination of calcium August 4 - 08:33 pm

Alendronic Acid Method August 12 - 12:53 pm

GMP Rules in manufacturing of drugs August 5 - 02:58 am

Enantiomeric Separation August 10 - 05:59 am

Determination of noise and signal-to-noise calculation in HPLC analysis: automized vs manual and which formula? August 13 - 04:00 am

ODS 2? August 7 - 11:22 pm

HPLC chromatographic results problem August 18 - 04:56 am

GPC quantitation of polymer mixtures without base line resolution August 2 - 08:15 am

FMOC - Derivatization August 3 - 09:08 am

LC/MS Results attachments August 2 - 11:54 am

250/300mm x 3um August 15 - 06:46 pm

Diclofenac poor peak shape July 29 - 05:26 am

Risendronate July 30 - 01:10 am

Amino Acid Analysis via UV! July 28 - 01:58 pm

Aromatic Hydrocarbon Fractionation by HPLC August 3 - 08:55 am

Contamination? August 17 - 04:40 pm

Theoritical plate July 29 - 08:31 am

What means TLC ? July 27 - 02:43 pm

HPLC intermediate/advanced training course August 6 - 07:39 am

Lichrospher 100 250-4 RP18e 5um July 29 - 08:11 am

Methyl paraben and sodium methyl paraben July 28 - 09:21 am

Mobile Phase Additive for Chelating Agent July 30 - 12:39 am

Do you use program technique often? July 28 - 03:52 am

MOBILE PHASE pH FOR ORGANIC ACIDS July 30 - 01:01 am

Autosample carrover? August 9 - 07:17 am

Salt in salt out? July 26 - 11:49 am

No HPLC solvent July 27 - 07:06 am

Retention time loss July 25 - 03:24 pm

Polymer problems August 12 - 06:01 am

Standard peak splitting after injection of sample ! August 6 - 12:48 pm

ChemStation files to ASCII files July 23 - 07:09 am

Choosing the best wavelength August 5 - 04:31 am

Solubility of impurities......... July 23 - 03:02 pm

Hitachi Amino Acid Analyzer L-8000 August 25 - 11:18 am

Epimers July 26 - 06:40 pm

Use of Poroshell packings for small-molecule separations July 25 - 03:37 pm

My new boss wants 100 +/- 0.1% accuracy!!!! July 22 - 12:37 pm

Stephanie July 29 - 01:38 am

Size exclusion resolution is poor July 26 - 03:45 am

Lc/MS Compatible Method for Paraquat and Diquat August 9 - 01:40 am

Need information about suppliers of bare silica gel with a pore size of 1000 angstrom! July 20 - 06:25 pm

Waters 2420 ELSD in THF July 21 - 02:42 pm

Estimating Column Backpressure July 20 - 03:12 am

About desfuroylceftiofur July 20 - 12:55 am

Industrial standard for LC column July 20 - 12:51 am

Lab equipment auctions August 5 - 04:47 am

Problem with Sample and Standard Stability July 19 - 06:08 am

Stability indicating method for Mitoxantrone July 19 - 06:44 am

The high and the area of peak July 19 - 09:23 am

HPLC conditions for basic compound? August 3 - 09:06 am

LC-MS August 3 - 09:09 am

Method development August 27 - 12:26 pm

Faster analysis of AccQ derivatised amino acids February 5 - 07:10 am

Wavy noisy baseline August 3 - 09:11 am

Cross-validation EP and USP August 3 - 09:13 am

Ion chromatography with chemical suppression August 10 - 10:29 am

HPLC Ambient Temperature? August 10 - 10:31 am

Problem with high pressure in HPLC system August 3 - 09:04 am

Force Degradation for LC Method Development August 10 - 10:36 am

Column use August 10 - 10:39 am

The Best HPLC system August 10 - 10:40 am

Unconventional HPLC method August 10 - 10:42 am

TFA at 210nm August 10 - 10:44 am

Linearity plot not passing through Zero August 10 - 10:45 am

"phase collapse" question August 10 - 10:50 am

Dissolution Method for Hyoscine Butylbromide ( Scopalamine ) Tablet !!!! November 17 - 04:54 am

Transfer of Triclosan from EtAc to ACN August 10 - 10:51 am

Ghostpeaks when injecting eluent November 19 - 08:25 pm

CARRYOVER ISSUES August 10 - 10:54 am

How to Calculate Relative Response Factor in HPLC Analysis? August 10 - 10:55 am

Metformin HCl and impurities method development August 17 - 08:03 am

Seperation of ethanolamine by LC August 10 - 10:57 am

Supplier for special substances December 1 - 07:04 am

Research Paper On Chromatography! Please Help! August 22 - 06:58 am

HPTLC method for Gatifloxacin August 22 - 11:04 pm

Analysis of risedronate sodium August 10 - 10:58 am

Polymorphism of Pharmaceutical compounds August 10 - 11:01 am

Articles about ibuprofen and diclovenac August 10 - 11:03 am

Signal-to-noise ratio and accuracy August 10 - 11:04 am

Separation of Catecholamines August 10 - 11:06 am

Who have chemstation and millinium32,can you give me a copy? August 10 - 11:08 am

Difference between accuracy and recovery August 10 - 11:09 am

Amino acid analysis August 25 - 12:39 pm

Vial Crusher August 10 - 11:11 am

Effect of concentration on plate height August 10 - 11:44 am

HPLC grade methanol August 10 - 11:46 am

HILIC of amino sugar August 10 - 11:47 am

EDDHA isomer separation August 10 - 11:49 am

Citrulline dosage August 10 - 11:51 am

Amine hydrochloride in HPLC August 10 - 11:53 am

What is your favorite procedure for cleaning up an LC? August 10 - 11:57 am

Titanium dioxide in sunscreens August 25 - 11:42 am

Source for columns August 10 - 12:00 pm

A proper internal standard for thiamine August 10 - 12:02 pm

Preservatives in cosmetic products August 10 - 12:06 pm

Peak height or peak area in HPLC analysis August 10 - 12:14 pm

Ion-pairing agent August 10 - 12:17 pm

TLC for oligosaccharides August 22 - 07:08 am

ACN/MeOH mixtures August 10 - 12:19 pm

Which column???? May 2 - 04:37 pm

Very poor retentivity of compound August 10 - 12:21 pm

Substitute for Beckman's Amino Acid columns? August 10 - 12:23 pm

Good column wash for C18 August 10 - 12:26 pm

Ketoconazole August 10 - 12:28 pm

HPLC internal standard August 10 - 12:30 pm

PKa of heptanesulfonic acid? August 10 - 12:31 pm

Analyst 1.4 August 10 - 12:33 pm

Vitamin D August 10 - 12:56 pm

Column washing August 10 - 01:01 pm

Hplc method development for detecting ascorbic acid August 10 - 01:03 pm

Dexchlorpheniramine maleate August 10 - 01:05 pm

Loperamide hydrochloride related substances - HPLC August 10 - 01:16 pm

Ghost peak problem May 19 - 10:48 am

Shikimic acid + HPLC August 10 - 02:53 pm

Separation of tartaric acid and oxalic acid August 10 - 03:11 pm

PTFE & Cellulose Filters August 10 - 03:17 pm

Ion Chromatography Solvent August 12 - 09:05 am

Baselining problem: A series of "V~V~V~V" baseline August 12 - 09:09 am

Difference in total impurity results by HPLC August 12 - 09:11 am

Lauryl Sulfate and Degassing August 12 - 09:26 am

LogP by HPLC August 12 - 12:20 pm

Column dead time??? August 12 - 12:22 pm

SEC Regeneration Procedure August 12 - 12:25 pm

Peak Splitting Phenomena June 11 - 12:13 am

Desloratadine August 12 - 12:28 pm

Help for separation condition of catecholamine!!! August 12 - 12:30 pm

Degradation of Antioxidants August 12 - 12:32 pm

Software Issue/ chromquest August 12 - 12:34 pm

Shimadzu LC2010 August 12 - 12:35 pm

HPLC METHOD DEVELOPMENT June 2 - 05:39 pm

Recovery software package (ACLJ) June 1 - 12:45 am

Problems with TSK-gel amide-80 column June 4 - 02:56 pm

Responce factor ,correction factor June 2 - 12:27 pm

"Control" standard for HPLC Assay determination June 3 - 04:32 pm

Help! PVP holds my peak tightly. June 4 - 11:21 am

Analytical to semi-preparative scale help June 1 - 04:16 pm

Analysis Method on HPLC for Midodrine, Oxcarbazepine and Cilostazol June 2 - 04:15 pm

Analysis of Anions by HPLC (water matrix) June 24 - 05:05 pm

HPLC method for Methane sulfonic acid June 11 - 07:39 am

Problems with static when weighing June 2 - 04:59 am

Xcalibur for two LC pumps June 1 - 06:07 pm

Peak tailing when using a Shimadzu HPLC system June 18 - 07:18 pm

Method development for glycosylated peptides June 4 - 03:03 pm

Proficiency testing June 8 - 03:29 am

Chromquest-relative standart deviation June 6 - 11:40 am

Ghost peaks on ELSD detection June 16 - 12:42 pm

Tamsulosin pellets with low concentration June 4 - 01:24 am

Ph. D in Canada June 16 - 05:42 am

Multiple charge and retention time for proteins June 5 - 09:47 am

Loperamide June 3 - 08:22 am

Separation of dihydroxynaftalen and its pyranoside June 4 - 03:16 pm

Hplc mobile phase June 6 - 05:22 am

Good website for method development June 5 - 09:58 am

Flow switcher June 5 - 10:11 am

SCX columns June 7 - 06:18 am

Methylene selectivity June 4 - 07:02 am

Good quantitative practice for drug quantitation? June 7 - 04:39 pm

Fluorescing agent in silicone lubricant June 4 - 04:26 pm

Validation parameters June 9 - 07:04 am

Necessity for buffers in HPLC August 25 - 05:26 pm

Imp�rities June 7 - 03:35 am

Resolution in RP-HPLC June 8 - 11:01 pm

Eluent composition in ion chrpmatography June 12 - 04:54 pm

Separation and identification of MBBT June 10 - 07:17 pm

Buprenorphine/Buprenorphine known imp. A June 7 - 06:33 am

Unknown Arginine Phosphate Buffer Peak in RP-HPLC?? June 7 - 07:56 am

LogD HELP WANTED!!!! June 7 - 08:40 am

Broad peaks June 12 - 07:53 pm

Formulate drug candidates for preclinical trials June 7 - 12:54 pm

Estimation of MBBT in SUNSCREENS June 7 - 10:41 pm

How to perform gradient valve pair test for a binary system June 8 - 07:09 am

Method Validation June 8 - 05:16 pm

Gentamicin isomer seperation June 9 - 01:07 am

Column dead time June 15 - 11:00 am

Plate count (N) proportional to Length (L) / particle diameter (dp) ?? June 11 - 04:29 pm

Regular interval in Mass June 11 - 03:22 pm

Generic protocol for drugs June 9 - 05:57 pm

Recent problems with analytical Waters XTerra RP 18 and MS coloums June 9 - 05:40 pm

Benzoic acid June 18 - 09:19 pm

Extraction method for Salicylic Acid August 25 - 11:54 am

Perfusion chromatography July 28 - 03:14 pm

Ion-paired AdoHcy/SAM HPLC detection June 9 - 12:34 pm

Analysis of vitamin A isomers (trans and cis retinol) June 10 - 10:14 pm

CONTINUOUS LIQUID/LIQUID EXTRACTION June 10 - 02:26 am

Itaconate assay? June 10 - 07:20 am

HPLC method for glycerin June 16 - 05:18 pm

Retention time smaller than t0 June 14 - 02:08 pm

Laboratory Instrument Qualification June 11 - 02:44 pm

The Waters Alliance Autosampler is not working June 23 - 02:26 am

Copper analysis by HPLC ? June 12 - 05:26 pm

Impurities in API by HPLC June 15 - 01:57 am

New board, with search options June 16 - 09:23 am

0.05 M Phosphate buffer June 21 - 06:54 am

Used HP 1040 or 1050 VWD HPLC detector needed, help June 14 - 06:42 am

Limit of Chloroacetic acid in API June 14 - 06:37 pm

Chemstation: use of std curve to determine ppm? June 16 - 02:39 pm

Strange pressure problem with a Beckman 126 pump June 16 - 12:18 pm

Tolterodine June 14 - 06:49 pm

Question on gradient elution in reverse-phase HPLC June 20 - 07:11 pm

HPLC method for sebum June 15 - 02:27 am

Sebum analysis by HPLC July 2 - 12:01 am

Amino acid analysis June 25 - 01:39 pm

Concentrations in HPLC June 16 - 06:05 am

Primer for Newbies? June 16 - 04:44 pm

Problems in analysing stearic / oleic acid June 22 - 11:44 pm

Signal-to-noise ratio June 22 - 06:16 am

Chromquest problem June 21 - 02:23 pm

Analysis of NaOH in a water sample June 25 - 04:08 pm

Problem with hold time when started a gradient elution June 16 - 07:50 pm

Variation in retention time due to variation in concentration June 20 - 06:48 pm

Does TFA 0,1% require a special column? June 23 - 02:09 pm

Operator's manual for Applied Biosystems 1000S DAD June 17 - 09:02 pm

Change in slope but retention time is the same June 18 - 06:12 am

Dependance of retention upon pH in IC June 21 - 08:36 am

Guard colums June 19 - 07:15 am

HPLC method and detection of peracids June 19 - 09:16 pm

RE Corosolic Acid HPLC method June 12 - 02:28 pm

Quinolin and Iso-quinolin in Naphthalin June 21 - 06:59 am

Availability of Allsep Anion IC column June 18 - 11:46 pm

SPE of very polar compounds June 23 - 02:08 am

Determination of paracetamol (acetaminophen) and ibuprofen by HPLC in human plasma matrix June 20 - 10:03 am

Choice of Sep-Pak cartridge June 21 - 02:28 pm

C18 Water for HPLC June 23 - 05:56 am

Cosmetics! June 21 - 08:26 am

Hplc of peptides June 23 - 10:23 am

Flavonoids in IAM column - any input is appreciated June 23 - 08:37 am

Boric acid June 27 - 12:26 am

Peak shape in HPLC-ELSD detection June 29 - 04:15 am

Does anybody know why ion trap is not good for quantitation? June 23 - 11:33 am

Calculating Error June 28 - 08:53 am

Variability problem in HPSEC June 25 - 12:15 pm

Chitin GPC June 22 - 07:28 pm

Ammonium Acetate Buffer June 23 - 05:44 pm

Buffer for pH 8? June 23 - 06:26 pm

GPC of glycopeptides June 23 - 02:13 am

HPLC method for acrylic acid (AA) and methyl methacrylate (MMA). June 23 - 08:11 pm

Related substances of stavudine July 1 - 01:44 pm

Ion exchange HPLC June 24 - 10:51 pm

What applications would benefit from an automated filtering and injection system? June 23 - 10:35 am

Waters Empower Software and Rheodyne injection problem June 25 - 07:10 am

Bizarre offscale peaks for DA following normal runs June 28 - 08:51 am

How to fix this HPLC method? June 25 - 06:29 am

Use of Quadratic Fit for Anions Analysis with Ion Chromatography June 28 - 12:31 am

Reduction of Peak Intensity June 28 - 01:07 am

Ion Chromatography June 29 - 01:37 am

Solubility related question June 25 - 11:04 am

HPLC method for acrylic acid (AA) and meth acrylicate (MMA) June 24 - 11:59 pm

This CPC assay doesn't work! June 30 - 08:16 pm

Gradient method developement at 210 nm . July 5 - 10:33 pm

Pump not mixing properly June 29 - 09:50 am

Empower July 1 - 01:39 pm

HPLC Peak Resolution July 7 - 08:46 am

The question for someone from LCGC advisory board June 30 - 08:35 am

Vapor Lock July 1 - 07:38 pm

Reverse Phase EDTA detection method June 30 - 01:21 pm

PH of Buffer in Affinity Chromatography-Glycated Hemoglobin July 3 - 12:04 pm

Recovery at the Border Point June 29 - 06:08 pm

HPLC/GC method for chloro propyl amine July 2 - 05:28 pm

Surfactant Analysis July 10 - 02:15 pm

Probe for silanol activity July 1 - 09:16 am

Help needed for gradient LC July 4 - 10:45 pm

How do I know if the system extra-column volume is significant? June 30 - 12:52 pm

Hypercarb Column and Chlorothalonil August 10 - 06:46 pm

Random Peak in DMF GPC July 1 - 04:41 am

Dihydrostreptomycin July 2 - 12:35 am

Calibrating HPLC July 2 - 02:48 am

GPC peak broadening problem July 25 - 04:23 pm

Where do all the Cys-containing peptides go? July 1 - 04:25 pm

ANTIOXIDANT PEAKS IDENTIFICATION BY HPLC-DAD July 8 - 03:02 am

Seeking contributors for new preparative TLC book July 2 - 10:00 am

Worm attractent July 7 - 06:45 pm

HILIC July 12 - 03:20 pm

Hydrocodone July 8 - 02:38 pm

Pyridine substitute July 13 - 02:02 am

Peak integration for poorly resolved impurities July 9 - 04:44 pm

How does helium acts to degass mobile phase? August 2 - 11:28 pm

Baseline drift August 15 - 07:17 pm

Quantifying arabitol July 8 - 11:29 am

Negative Peaks in Chromatogram July 8 - 04:34 am

Profiling proteins July 9 - 12:22 am

Retention time gets shorter and shotrer... July 8 - 01:35 pm

Negative RI peak? July 20 - 10:05 am

Change in retention time and baseline July 9 - 09:58 am

Chiral chromatography - Ibuprofen July 12 - 06:44 am

HPLC method for Tretinoin (all trans retinoic acid) July 8 - 06:36 pm

User Requirements for HPLC July 16 - 11:52 am

Stability Indicating Method July 9 - 12:21 am

Automatic solvent switching for high throughput LC-MS July 8 - 06:27 pm

Analysis PFOS IN WATER SAMLE July 12 - 10:09 am

Herbicide analysis July 15 - 07:40 am

Waters Alliance bubble recovery sequence July 14 - 06:33 am

Flow cell and sensitivity July 25 - 04:27 pm

What causes negative peak in LC using UV detection? July 11 - 06:46 am

The HPLC method development bible July 12 - 10:00 am

Polyglycol acetate July 17 - 11:37 am

Electrochemical Detection in HPLC August 11 - 09:29 am

Sample Dilution Problem July 13 - 12:34 am

Quantification in IC - Forcing the cal. curve through zero or not? July 13 - 12:36 pm

Graphite Columns July 12 - 03:44 pm

Sulfonyl Urea of LCMSMS July 16 - 02:24 pm

TOCOTRIENOL & ORYZANOL STANDARDS July 14 - 12:57 am

HPLC ANALYSIS OF HEPARIN July 13 - 10:22 am

LOD and LOQ according to Ph. Eur. July 16 - 10:30 pm

AMINOACIDS HPLC August 25 - 05:08 pm

Retention time July 15 - 03:08 am

Hi,I am using Prep HPLC- Facing PROBLEMS .Can any body suggest the solution for Aqueous 0.1% TFA with ACN (50:50)created air lock in Prep HPLC August 1 - 08:13 am

Lactic Acid Analysis: Mono-, Di- and Trimers July 20 - 11:50 am

Reversing a column!!!!! July 15 - 01:19 pm

Instrument problem with Finnigan TSQ 700 July 15 - 07:41 am

THF/MeOH needle wash July 25 - 04:19 pm

Agilent autosampler carry over August 24 - 10:55 am

Cation suppression agents in IC July 25 - 04:45 pm

Glyphosate impurities July 16 - 11:31 am

CHEMSTATION HPLC 1090 Rev.A.03.02 July 19 - 08:47 am

Hplc method development July 20 - 12:06 am

pH and retention July 23 - 08:23 am

Some Basic Questions August 9 - 10:46 am

Silica vs zirconia vs titania column for HILIC August 1 - 10:17 am

Instrument Calibration August 3 - 10:05 am

DONEPEZIL Dissolution Test August 9 - 10:06 am

Sample preparation July 20 - 02:30 pm

Derivitization of Tranexamic acid August 11 - 08:01 am

Method Validation guide for Dequalinium Chloride Tablet August 15 - 07:13 pm

Cosmetic serums August 20 - 04:42 am

Formaldehyde with HPLC-UV or headspace GC-MS/FID August 27 - 09:57 am

Welcome to LC Archives. You may read any of the discussions above by clicking on the appropriate link. To start a new discussion of your own, Go to the LC Message Board.