By Nick Baetz on Sunday, October 8, 2000 - 12:49 pm:
Tried separation of vitamins in Centrum and could not get good results. 254nm, 30-70 MeOH and Buffer(KH2PO4),C-18. Separations are not easily optimized. Can't think of whatelse, other than mobile phase might be wrong. Appreciate help.
By hinsbarlab on Tuesday, October 10, 2000 - 07:27 am:
Welcome to the wonderful world of chromatography. Not so easy, is it? Many, many people have spent a lot of time and money developing chromatographic methods for vitamins in tablets and many, many articles have been published dealing with this subject. A literature search would be a good starting point.
Best Regards,
Michael Hinsberg
http://www.hinsbarlabs.com
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