By Anonymous on Tuesday, October 10, 2000 - 06:48 am:
I am looking for a good reference book that would describe the effects of cis vs trans on UV spectra (I have a couple already, but they don't emphasize that particular topic).
Right now, I have a trans molecule with a UV max at 329 nm (shoulder at around 290nm). I believe I found the cis isomer (UV: Max at 285nm, plateau at 329nm) of that molecule. LC-MS spectra show identical fragmentation patterns, but with different intensities of specific fragments.
Thanks a lot for your inputs.
By Anonymous on Wednesday, October 11, 2000 - 10:06 am:
You might do better to use NMR.
By Anonymous on Thursday, October 12, 2000 - 04:26 am:
I don't mean to be sarcastic, but I kind of knew that (about NMR). I may have to explain my request a bit more: we are using LC with a PDA and ELSD, mounted in series. We acquired MS data on isolated fractions of each "isomer". So far, the MS spectra are identical in fragmentation patterns, but, as I said earlier, individual peak intensity ratios do vary significantly.
My question was: is there any way to confirm the cis/trans isomerism by UV?
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