Preparation of protein samples for amino acid analysis??

Chromatography Forum: LC Archives: Preparation of protein samples for amino acid analysis??
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Friday, October 20, 2000 - 02:12 pm:

What is the simplest way to prep solid samples for amino acid tests by HPLC. I have a Waters Module 1, variable wavelength UV detector. I would have to do derivitization (pre-analysis)some sort.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Friday, October 27, 2000 - 07:52 am:

What exactly is your question? Do you have a pure sample of a protein you wish to digest for total amino acid analysis? Are you looking for an amino acid derivatization method?


Top of pagePrevious messageNext messageBottom of pageLink to this message  By PGPG on Saturday, October 28, 2000 - 07:03 pm:

Hydrolisis

HCL 6N 1% Phenol 110°C 24 Horas

With Nitrogen atmosphera.

I recomend PITC aa


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Friday, November 3, 2000 - 02:55 pm:

yes to both. Sample would probably be aninmal feed of some sort. I'm looking more for a good prep method more than an analysis method. Thanks for asking


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Tuesday, November 7, 2000 - 10:00 am:

Thanks PGPG Might have more questions later


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