What is the simplest way to prep solid samples for amino acid tests by HPLC. I have a Waters Module 1, variable wavelength UV detector. I would have to do derivitization (pre-analysis)some sort.
![]()
![]()
![]()
![]()
By Anonymous on Friday, October 27, 2000 - 07:52 am:
What exactly is your question? Do you have a pure sample of a protein you wish to digest for total amino acid analysis? Are you looking for an amino acid derivatization method?
![]()
![]()
![]()
![]()
By PGPG on Saturday, October 28, 2000 - 07:03 pm:
Hydrolisis
HCL 6N 1% Phenol 110°C 24 Horas
With Nitrogen atmosphera.
I recomend PITC aa
![]()
![]()
![]()
![]()
By Anonymous on Friday, November 3, 2000 - 02:55 pm:
yes to both. Sample would probably be aninmal feed of some sort. I'm looking more for a good prep method more than an analysis method. Thanks for asking
![]()
![]()
![]()
![]()
By Anonymous on Tuesday, November 7, 2000 - 10:00 am:
Thanks PGPG Might have more questions later
Posting is currently disabled in this topic. Contact your discussion moderator for more information.