By M. Rieberg on Monday, October 23, 2000 - 07:51 am:
I am trying to find residual toluene in a relatively non-polar substance. I should use a normal phase column, right? Any reccomendations on columns and eluents?
By Anonymous on Monday, October 23, 2000 - 06:23 pm:
The relevant question is if the "relatively non-polar substance" is soluble in a RP solvent such as acetonitrile or methanol, and if it is expected to stick to an RP column or not. If the sample is soluble, and if the "substance" does not stick to strongly to the C18, a RP method is preferred.
By M. Rieberg on Tuesday, October 24, 2000 - 10:46 am:
The relatively non-polar will disolve in THF and acetone as far as RP solvents go, (those are acceptable RP solvents?). It's most soluble in something like toluene or hexane, hence the leaning toward a np method. Would a THF/Water be acceptable here for an RP method?
By Anonymous on Tuesday, October 24, 2000 - 02:37 pm:
THF-water is perfectly fine. The RP method is preferred due to its insensitivity to retention changes from mobile phase composition changes. NP is very sensitive to water content in the solvents.
Posting is currently disabled in this topic. Contact your discussion moderator for more information.