Sample Prep-Centrifuge Speed

Chromatography Forum: LC Archives: Sample Prep-Centrifuge Speed
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Friday, October 27, 2000 - 04:20 am:

Hi, everyone!

I leart recently that rpm is NO LONGER a valid international unit for centrifugation speed. Instead relative centrifuge force g is used. Is this true? If so, how to calculate g?

Thank you very much in advance.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Tom M. on Monday, October 30, 2000 - 04:46 pm:

The relative centrifugal force (RCF) = .00001118 x r x N^2. Where r is the radius of rotation in cm and N^2 is the square of the speed
of rotation in revolutions per minute (rpm).

Thus for a 10cm rotor at 3000rpm:
RCF = .0000118 x 10 x 30000 x 3000 = 1006 g

I don't know about any international units but it seems to me if you write the RCF into the test method and give the equation then anyone will be able to run the method regardless of what type of centrifuge they have. Good luck.


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