By SawHong on Monday, October 30, 2000 - 11:11 pm:
I'm analysing glyphosate and AMPA using HPLC post column with the fluorescence detector.
The mobile phase is 5mM phosphate buffer + 4 % MeOH pH2.1 at 0.5ml/min.
Column: Cation exchange column
The peaks of Gly and AMPA are both 4 minutes broad even at low concentration.
Is there any suggestion to decrease the width of these peaks?
Detector: Ex 340nm Em 455nm
Is it possible the reading of the detector will affect the width of the peaks?
Thanks for any suggestions.
By Anonymous on Friday, November 3, 2000 - 03:14 pm:
It appears that your post column derivatization is killing you. How are you doing it?
By B.Buglio on Wednesday, November 8, 2000 - 05:19 pm:
How does this question relate to that of R.Ryser
on Oct. 24th? In any event Waters used to sell a
post column amino acid derivatization module
applicable to this application (its the glycine
thats being derivatized). Either buy the unit or
get the specs as related to:(1) dead volume of the
connecting tubing,(2)reactor and (3) detector then
compare to your set-up. Another approach is to do
this assay by CIA using a high mobility anion
mobile phase. Jim Krol at Waters has a CIA
glyphosate application with this system.
By B.Buglio on Wednesday, November 8, 2000 - 05:25 pm:
Opps- sorry the above should read "dead volume of:
(1) connecting tubing (2) reactor and (3)
detector". I agree w anon - its probably the dead
volume in the reactor train thats killing you.
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