Can anyone help me out?? I am trying to find any information on Single Point Calibration vs. Running full Cal. Curve for every LC run. I am trying to increae throughput in my lab and trying to form an argument for the merits of running a Validation with linearity and then using single point calibrations for all Assay runs. My higher ups are wary of it because a lot of our samples vary in concentration going to extremely low levels (average area response <10 area counts)

The worry is that if the lamp is starting to go, the low end is what it would be most detrimental and you would never know if you didn't run a full Cal curve.

If I could do single point I could save 8-12 hours per run.

Is it acceptable to run single point calibration and then an LOD/LOQ STD to varify the response is suitable at lower levels.

If anyone could help me with this or where I could find literature to support my thoughts, I would grweatly appreciate it.

I would like to suggest several solutions which might help but I need a little more detail about your situation to determine if any of my ideas are applicable.
How many components are you looking for? What is the time of elution for the last analyte of interest? How long is each chromatographic run? How many different concentrations are you using for your calibration curve data set? What is the dynamic range for the calibration standard curve? How many injections per calibration standard? Is there any cleaning or flushing time between injections and if so for how long. Are your runs isocratic or gradient? If gradient how long is the total run including any equilibration time? Do you have an auto-sampler or are all injections manual?
I have recently used a 7 point external standard calibration set, in triplicate for determination of 4 alcohols ranging from 10ppm to 500ppm of each. Previously, I have used a 10 point external standard calibration set, in triplicate, for a single component, ranging from 1ppm to 1000ppm. In both cases I had a large number of samples and my supervisor was concerned about not being able to reproduce, area response to concentration ratio, over the entire sample set. I was able to run the entire calibration set only once and show during sample analysis that the calibration curve was valid over the entire run.

Single point calibration assumes the y-intercept is zero and the slope is the response determined for your single standard. Single point calibration is really only applicable when the sample and standard concentrations are very close to one another. However, each situation is different and you will need to experimentally determine if you can get away with single point calibration over the whole range. This will depend upon the accuracy required for your result. If single point works, then using LOD/LOQ standards at the upper and lower limits seem reasonable. However, if you're going to do that, why not run a three point calibration instead?