By Anonymous on Thursday, December 21, 2000 - 05:08 pm:
What would be the cause of retention time shift of the second eluter when the retention time of the first eluter remains the same? The retention time increased about 13% for 40 injections. It was an isocratic run with mobile phase of 40% IPA, 60% ACN and 0.1%water.
By Anonymous on Friday, December 22, 2000 - 08:45 am:
What is the column? What is the retention time of the first peak? What is the flow rate?
By Anonymous on Friday, December 22, 2000 - 09:26 am:
It is a Kromasil C18 column. The retention time of the first peak was about 7 min and the second peak started at 10 min.
By Anonymous on Friday, December 22, 2000 - 01:15 pm:
You still did not tell us the column dimensions and the flow rate.
Why are you using nearly 100% organic on a C18 column?
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