By Anonymous on Thursday, January 11, 2001 - 06:33 am:
Hello,
Could anybody please explain the effect of pH on the separation of different compounds on reverse phase columns. How do I distinguish between ionic and neutral compounds?
Thanks in advance!
By Anonymous on Tuesday, January 16, 2001 - 10:53 am:
I advise you attend some of the seminars by LC resources.
By Mike McBrien on Thursday, January 18, 2001 - 01:10 pm:
The key actually lies in the pKas for ionizable sites. If you know the pKas, then you know if the species is ionic or neutral at that pH. If you do not, then there is software to indicate this, such as our product, ACD/LC Simulator. It predicts pKas and hydrophobicities, then uses these to predict retention times.
Please see our web site for more details:
www.acdlabs.com
Best regards,
Mike.
Mike McBrien, Product Manager
Advanced Chemistry Development
By Rudy on Monday, April 16, 2001 - 12:56 pm:
If you have time alter the pH of your mobile phase. You will notice your peaks may get reversed in their position or lump under one peak. pH has a lot to do with the separation of your peak.
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