By Anonymous on Thursday, January 18, 2001 - 01:29 pm:
I'm looking for a method to analyze these two compounds by HPLC. Alpha lipoic is also called thioctic acid. Any suggestions????
By Anonymous on Tuesday, January 23, 2001 - 12:59 am:
Take a look at this method :
column - nucleosil 100-7 C18 120x 4.0 mm
eluent - acetonitrile/phosphoric acid 0.01 M
gradient : t0' 25 % CH3CN
t15' 40 % CH3CN
UV - 210 nm
flow - 2.0 ml/min
sample concentration - 0.4 mg/ml
temperature - 35°C
injection volume - 20 ul
By Mike McBrien on Tuesday, January 23, 2001 - 08:03 am:
Here is a method retrieved from the Applications Database in ACD/ChromManager:
column - Chromspher UOP 1.5 C18
eluent - methanol/0.05 M Na-octanesulfonate,
0.005 M Et3NH-phosphate
gradient: t0' 5% MeOH t2' 15% MeOH
UV - 220 nm
flow - 1.0 mL/min
temperature - 30 C
The method is based on thioctic acid, not Pregnenolone.
ACD/LC Simulator predicts a long retention time for Pregnenolone, however. You may wish to run the gradient up to a higher % MeOH based on this.
Hope that this helps.
Best regards,
Mike.
********************************************
Michael McBrien M.Sc.
Product Manager
Advanced Chemistry Development
90 Adelaide Street W. Suite 702
Toronto, Ontario, Canada M5H 3V9
michael@acdlabs.com
416-368-3435 X235
www.acdlabs.com
********************************************
Posting is currently disabled in this topic. Contact your discussion moderator for more information.