By Anonymous on Friday, January 19, 2001 - 04:51 am:
I want to compare the Area Under Curve for some peaks which I don't have standards for. Sometimes this value is in the millions. What is a convenient way to report the AUC and what units should I show? I think that the units for AUC are (absorbance * time)
thanks for any help.
By chromgod on Wednesday, January 24, 2001 - 08:07 am:
This is a very vague question. What do you want to compare the area to? I suggest you look up information on internal standards. This will allow you to compare it to something at least.
By Anonymous on Wednesday, January 24, 2001 - 12:22 pm:
Chromgod, sorry for the vague question. Say I inherited some data from a former labmate, and absent of internal (or external standards)I want to compare the area of a peak from one run to the area of the same peak from another run. The only units I have are AUC, so if one is smaller than the other, I could express one as a percentage of the other? or a % change in the areas? I do know injection volume and initial protein concentration of the samples was the same, so any change in peak size would be related to the experiment at hand. I just would like an alternative to comparing 600,000 AUC to 5,000 AUC and saying what % change was observed. Any help is appreciated.
By Anonymous on Wednesday, January 24, 2001 - 06:31 pm:
There is in principle nothing wrong with using the straignt absorbance*time....
Posting is currently disabled in this topic. Contact your discussion moderator for more information.