I need a Spray Reagent specific for thiol (SH-)group. It should to be able to detect low amounts of thiol group (0,2 µg: 5 µL x 0,05 mg/mL)
Does anyone has anny idea ?
Thanks
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By kiilkl on Tuesday, March 30, 2004 - 08:17 am:
I need a composition of IODOPLATINATE spray reagent for detection of Sulfur Compounds (Awwad HK, Adelstein SJ 1966 A quantitative method for the determination of the specific radioactivity of sulfur-containing amino acids separated by paper chromatography. Anal. Biochem. 16: 433-437.)
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By Anonymous on Tuesday, March 30, 2004 - 11:16 am:
Try a classic book.
Stahl, Ergon "Thin Layer Chromatography", 1st. Ed. (1969).
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By Anonymous on Tuesday, March 30, 2004 - 11:41 am:
Iodoplatinate
0.15% w/v potassium chloroplatinate and 3% potassium iodide in dilute hydrochloric acid.
For detecting alkaloids, amines, and organic nitrogen compounds.
Is this it?
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