Can anybody help me in choosing a chiral column ?

I don't have any experiences with these columns, so I don't know which column would be the best.
I know I could go through hundreds of applications, but I get such a broad range of substances to purify that it's really difficult to specify:
I need a column which is "multifunctional" (70 % of my substances are basic and most of them are amines, the rest of it...?). I don't want to use buffers (if I can avoid it) and I heard of people who haven't made good experiences with reversed phase chiral colums.

Does anybody of you have experiences with such a multifunctional column ? Can you tell me what column you use and for which functional groups ?

I know it's all a bit vague, but I would be very glad about every information and help.

Hi Sibylle,

I think that the type of columns that you need are the peptidic antibiotics based chiral stationary phases commercialised by astec (USA). They have 3-4 types of such columns that have complementary chiral selectivities one from another. Normaly you work with mobile phases containing water-methanol or acetonitrile (high concentrations of organic modifiers).
There are 4 principal chiral columns that you can do up to 80% (personal opinion) of chiral compounds:
Chirobiotic V, R, T, TAG

Try their web site and do not hesitate to ask for their help (opinion) concerning your compounds.

Hope that the above helps

Kostas

Hi Kostas,
thank you very much for your advice. I 've found some very interesting things on their website...
Sibylle

hi Sybille,

I work for a few months with DAICEL colums, these are the most popular colums to separate racemates ( approximatively 80% of the existing racemates)
The name of these are Chiralcel OD and Chiralpak AD. With these columns, we use normal mobile phases ( ex: Hexane / Propanol ) but the solubility of the analytes is often a limiting factor, because they are less soluble in such eluant, and Chloroform, Dichloromethan, DMSO, .... can solubilise the stationnary phase.
Cordially

My problem is not specifically column, but I think that is possible too. I'm tryng to separate the enantiomers of haloxyfop and fluazifop (acid forms) with Pirkle column (covalent). Using MP 2-propanol (1%) and Hexane I separated both enantiomers of Fluazifop butil (the resolution is not good). First of all, I would like to know how to improve the resolution of both enantiomers. Lowering the concentration of alcohol or adding acid like TFA? I think if I get the separation of enantiomers, certainly I will do the derivatization of acid form of these herbicides to use the Pirkle column. In this case the use of BF3 is a good method? Tanks a lot for help me