By Anonymous on Wednesday, March 14, 2001 - 10:27 am:
I found a literature reference to a separation that I would like to perform. The mobile phase used in the reference is a 25 mM phosphate buffer/acetonitrile gradient. Could anyone give me a procedure for preparing the 25 mM phosphate buffer? Also from the way it was stated in the reference, I assume the buffer is only added to the water and not to the acetonitrile. Is this correct? Thanks in advance for your assistance.
By I.D. on Wednesday, March 14, 2001 - 10:45 am:
Try this:
http://www.bi.umist.ac.uk/users/mjfrbn/buffers/makebuf.asp
By Merlin on Wednesday, March 14, 2001 - 04:33 pm:
Dissolve 25 mmole (FW x 0.025) phosphate in 1 L water. Adjust pH if necessary. If the method has pure MeCN as the B mobile phase, there definitely is no phosphate in it as it won't dissolve.
By H W Muller on Wednesday, March 14, 2001 - 08:59 pm:
Check"Alcohols and pH" February 6, 2001
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