I`ve tried some mobile phases (MeOH /water mixtures) on C18 column. Also I tried a NH2 columns (Hypersil APS Hewllet Packard). The model sustems were various: D,L-arginine, D,L-methionine, D,L-hystidine, D,l-lysine as aminoacids and glucose as reducing sugar. I know that, teoretically, it is possible to obtain in that kind of mixtures a lot of different compound (function at reaction parameters, e.g pH and temperature) but all I could obtain were some chromatograms with 3-4 peaks cramped in first 2-2.5 minutes range (inspite of using mobile phases with very low concentration of MeOH - about 5%).
I would be happy if you will share me some optimal conditions in working with C18 column and MeOH/Water mobile phases in order to obtain a resonable retention of interest compounds and some ways for positive ID of peaks in absence of suitable standard substances (I also mention I don`t have an PDA, DAD or MS detector, only a programable wavwlength UV detector).Thank you.